Composição química e atividade biológica de extrato oleoso de própolis: uma alternativa ao extrato etanólico

Buriol, Lilian; Finger, Daiane; Schmidt, Eduardo Morgado; Santos, Júlio Murilo Trevas dos; Rosa, Marcos Roberto da; Quináia, Sueli Pércio; Torres, Yohandra Reyes; Santa, Herta Stutz Dalla; Pessoa, Cláudia; Moraes, Manoel Odoríco de; Costa-Lotufo, Letı́cia V.; Ferreira, Paulo Michel Pinheiro; Sawaya, Alexandra Christine Helena Frankland; Eberlin, Marcos N.

doi:10.1590/s0100-40422009000200006

Cited by 61 publications

(35 citation statements)

References 29 publications

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“…O teor de compostos fenólicos do extrato de própolis marrom deste ensaio é semelhante aos 2,61% (26,1mg/mL), encontrado no extrato de própolis proveniente de Prudentópolis, PR analisado por Buriol et al (2009), entretanto o teor de flavonóides é superior aos 0,46% (4,6mg/mL). Há de se destacar que ambos os extratos estão dentro dos limites estabelecidos pela legislação brasileira, na qual o teor de flavonoides da própolis é de 0,25% -mínimo (2,5mg/mL) e para os compostos fenólicos 0,50% -mínimo (5,0mg/mL) (Brasil 2001).…”

Section: Resultsunclassified

Atividade antibacteriana in vitro da própolis marrom

et al. 2016

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RESUMO.-Objetivou-se avaliar a atividade antibacteriana in vitro da própolis marrom, por meio da determinação da Concentração Inibitória Mínima (CIM). O extrato alcoólico de própolis foi obtido de 35 g de própolis bruta macerada em 65 mL de álcool de cereais. As concentrações do extrato de própolis usadas foram: 75mg/mL; 56,4mg/mL; 37,5mg/mL; 18,9mg/mL; 9,3mg/mL; 4,5mg/mL e 2,25mg/mL. INTRODUÇÃOA própolis é uma substância resinosa que as abelhas coletam de diversas partes das plantas, como brotos, botões florais, cascas e exsudatos resinosos (Park et al. 1998, Pereira et al. 2002. As abelhas utilizam a própolis para fechar pequenos orifícios, mumificar insetos que morrem no interior da colmeia, defender contra invasão de microrganismos, fortalecer os favos e proteger a entrada das colmeias The aim of this study was to evaluate the in vitro antibacterial activity of brown propolis, by determining the minimum inhibitory concentration (MIC). The alcoholic extract of propolis was obtained from 35g of crude propolis macerated in 65mL of cereals alcohol. Dilutions of propolis extracts used were: 75mg/mL; 56.4mg/mL; 37.5mg/mL; 18.9mg/mL; 9.3mg/mL; 4.5mg/mL, and 2.25mg/mL. Thirty-two Gram-positive bacteria isolates were used: Rhodococcus equi, Staphylococcus aureus, Staphylococcus hyicus, Staphylococcus spp. and Streptococcus spp.; and the thirty-two Gram-negative bacteria isolated were: Enterobacter agglomerans, Escherichia coli, Klebsiella pneumoniae, Klebsiella sp., Pseudomonas aeruginosa, Pseudomonas sp., Salmonella sp., and Serratia rubidaea, obtained from infectious clinical processes of domestic animals, and stored in the Laboratory of Bacteriology of FAMEZ/ UFMS. The brown propolis extract showed antibacterial activity with MIC ranging from 2.25 to 18.9mg/ml for Gram-positive bacteria and 4.5 to 18.9mg/ml for Gram-negative bacteria, and bacteria from cattle and dogs were more resistant. It was concluded that brown propolis has antibacterial action, but the effect depends on the species of the bacterium and its origin.INDEX TERMS: Biological activity, brown propolis, bacteria, bactericidal, minimum inhibitory concentration.

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Section: Resultsunclassified

Atividade antibacteriana in vitro da própolis marrom

et al. 2016

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“…Interference from other phenolic compounds is frequently present, as the Al 3+ cations form stable complexes with free hydroxyl groups of lavonoids. This causes the extension of the conjugated system and consequently a bath chromic shift, or in other words, a shift of the absorption maxima to a longer wavelength region, allowing quantiication in a spectrophotometer at 425 nm [52].…”

Section: Total Phenolicsmentioning

confidence: 99%

Techniques for the Evaluation of Physicochemical Quality and Bioactive Compounds in Honey

Sereia¹,

Março²,

GeraldoPerdoncini³

et al. 2017

Honey Analysis

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Honey is a concentrated aqueous solution of sugar, especially glucose and fructose, and minor amounts of dextrin, enzymes, waxes, volatile oils, organic acids, ethers, albuminoidal gum substances and minerals. Commercially available honey samples vary in quality according to various factors such as climate diversity, type of lora of the surrounding region, geographical characteristics, processing, loral supply period, and packaging and storage conditions, which can compromise the standardization and quality of the inal product. The diferent techniques that will be presented in this chapter to assess the quality of honey are tests required by identiication standards and national and international quality control or are important quality tools that can be used in the evaluation of the conditions for obtaining and processing of the honey, fraud identiication and changes to and/or adulteration of the honey, ensuring the physical and chemical composition of the project and guaranteeing quality standards, directly impacting the shelf life and use and presentation of the product.

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“…All propolis samples, independent of their source, present antimicrobial activity because this material must protect the hive (Buriol et al, 2009). The biological activity of Brazilian propolis is mostly due to the high levels of phenolic acids (Laskar et al, 2010), whereas flavonoids are considered to be responsible for the activity of extracts from European propolis (Hegazi et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Chemical markers and antifungal activity of red propolis from Sergipe, Brazil

Mendonça

Araujo

et al. 2015

Food Sci. Technol (Campinas)

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The aim of this study was to analyze the physicochemical properties and antifungal activities of the red propolis samples from Sergipe, Brazil, and also evaluate their variability throughout the year. The characterization of the hydroalcoholic extract (HPE) of the red propolis samples was performed monthly from Dctober 2009 to September 2010. The concentrations of the bioactive compounds varied during the year, but their chromatographic profiles were similar. Four compounds were identified by comparison with authentic standards. Formononetin was one of the predominant compounds in all propolis extracts. In our study, it was observed that all the propolis samples inhibited the growth of Candida species. Multivariate analysis confirmed the variations in chemical composition and color of the HPEs throughout the year. The biological activities of the HPEs were statistically significant (p<0.05), and all samples exhibited antifungal properties.

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Composição química e atividade biológica de extrato oleoso de própolis: uma alternativa ao extrato etanólico

Cited by 61 publications

References 29 publications

Atividade antibacteriana in vitro da própolis marrom

Atividade antibacteriana in vitro da própolis marrom

Techniques for the Evaluation of Physicochemical Quality and Bioactive Compounds in Honey

Chemical markers and antifungal activity of red propolis from Sergipe, Brazil

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