Estrategias en el desarrollo de métodos analíticos para la determinación simultánea de compuestos orgánicos por espectrofotometría derivada

Toral, M. Inés; Orellana, Sandra L.; Saldías, Marta; Soto, César

doi:10.1590/s0100-40422009000100043

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…The measurement was limited in range from 190 to 350 nm, since outside it there is no absorption. The software identifies the factors that can be used [27] 11 times, increases the analytical signal and the background noise in the same proportion [28]. This factor only affects the attainment of a good reading of the analytical signal since it does not increase sensitivity.…”

Section: Selection Of Spectral Variablesmentioning

confidence: 99%

Simultaneous Determination of Piperacillin and Tazobactam in the Pharmaceutical Formulation Tazonam® by Derivative Spectrophotometry

Toral

Nova-Ramírez

Nacaratte

2012

J. Chil. Chem. Soc.

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In this paper, a simple and rapid method was developed for the simultaneous determination of Piperacillin (PIP) and Tazobactam (TAZ) by second-order derivative spectrophotometry. Water was selected as a solvent for extraction and determination of both analytes. Further studies of photo-stability and forced degradation for both drugs were also performed.The spectral variables were optimized, a smoothing factor of 8•10 3 and a scaling factor of 1•10 4 were selected and the analytical signals were evaluated at 296.3 nm and 233.3 nm for PIP and TAZ, respectively.The detection and quantification limits for PIP and TAZ were: 3.2•10 -7 to 9.5•10 -7 and 2.7•10 -7 to 8.9•10 -7 mol/L, respectively. The levels of repeatability (RSD) were 1.5% and 2.4% for PIP and TAZ respectively.The method was applied to the pharmaceutical formulation TAZONAM NF® Wyeth. USA, with a nominal content of 4.0 g of PIP, 0.5 g of TAZ, 0.139 g of citric acid and 1 mg of EDTA. A study of excipients was carried out and it was found that they do not interfere with quantification. By applying this method, a content of 3.96 ± 0.04 g and 0.57 ± 0.01 g was found of PIP and TAZ, respectively.

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Section: Selection Of Spectral Variablesmentioning

confidence: 99%

Simultaneous Determination of Piperacillin and Tazobactam in the Pharmaceutical Formulation Tazonam® by Derivative Spectrophotometry

Toral

Nova-Ramírez

Nacaratte

2012

J. Chil. Chem. Soc.

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“…3(II) are shown the derivative spectra of the same drugs in phosphate buffer pH 7.0 in presence of EDTA; therefore, the first and second derivatives could be used for analytical purposes. When the derivative order increases, sensitivity concomitantly decreases (Toral et al 2009). In this context, when the first-order derivative was used, the simultaneous determination of OTC and OA can be easily carried out because the spectrum shows well-defined areas for determining each analyte with high sensitivity.…”

Section: Optimization Of Spectral Variablesmentioning

confidence: 99%

Extraction and Determination of Oxytetracycline Hydrochloride and Oxolinic Acid in Fish Feed by Derivative Spectrophotometry of First Order

et al. 2011

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In this work is proposed the extraction and determination of oxytetracycline (OTC) and oxolinic acid (OA) in fish feed by first-derivative spectrophotometry. The extractions are carried out by parallel modality, where OTC is extracted in the presence of OA in a sample, and in another is extracted OA in the presence of OTC, and the sequential modality, where OTC is extracted first and then followed by OA in a single feed sample. A phosphate buffer, pH 7.0, was selected for OTC extraction and acetonitrile for OA extraction. These solvents were used in both extraction modalities. The extraction percentages obtained by parallel mode are better than those obtained by sequential extraction. In both cases, the limits of extraction were 25 mg kg −1 for OTC and 10 mg kg −1 for OA. However, it is proposed to work with the parallel extraction for its efficiency, accuracy, precision, and less time requirement.

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Carbon Nanotube‐Based Molecularly Imprinted Voltammetric Sensor for Selective Diuretic Analysis of Dialysate and Hemodialysis Wastewater

et al. 2020

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A molecularly imprinted polymer (MIP)‐based electrochemical sensor was developed for the monitoring of hydrochlorothiazide (HCT) in dialysate and hemodialysis wastewater. The MIP was constructed by the electropolymerization of ortho‐phenylenediamine on multi‐walled carbon nanotube modified glassy carbon electrode surface. The experimental optimization was carried out by Plackett‐Burman design screening and central composite design. Through square wave voltammetry (SWV) analysis, the sensor presented linear response in the range of 5.0×10−6 to 1.0×10−4 mol L−1 (R=0.9995) and detection limit of 3.2×10−6 mol L−1. The film morphology was investigated by SEM, EDX and AFM, and the electrochemical response was confirmed by EIS. The MIP film exhibited excellent selectivity for HCT, rapid response and good practicability. The sensor was successfully applied for direct and selective determination of HCT in dialysate and hemodialysis wastewater samples with recovery rates ranging from 97 % to 102 % based on the standard addition method.

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Estrategias en el desarrollo de métodos analíticos para la determinación simultánea de compuestos orgánicos por espectrofotometría derivada

Cited by 6 publications

References 18 publications

Simultaneous Determination of Piperacillin and Tazobactam in the Pharmaceutical Formulation Tazonam® by Derivative Spectrophotometry

Simultaneous Determination of Piperacillin and Tazobactam in the Pharmaceutical Formulation Tazonam® by Derivative Spectrophotometry

Extraction and Determination of Oxytetracycline Hydrochloride and Oxolinic Acid in Fish Feed by Derivative Spectrophotometry of First Order

Carbon Nanotube‐Based Molecularly Imprinted Voltammetric Sensor for Selective Diuretic Analysis of Dialysate and Hemodialysis Wastewater

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