Co-produção de lipase e biossurfactante em estado sólido para utilização em biorremediação de óleos vegetais e hidrocarbonetos

Martins, Vilásia Guimarães; Kalil, Susana Juliano; Costa, Jorge Alberto Vieira

doi:10.1590/s0100-40422008000800005

Cited by 25 publications

(28 citation statements)

References 17 publications

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“…The extracellular enzyme production is a positive factor when there is an intention to explore biotechnologically microorganisms. In this way, the importance of lipolytic production in an extracellular form is due to facility to obtain enzymes and lower cost for isolation (Saxena et al 2003, Martins et al 2008. Several factors can influence the extracellular microbial production, mainly because of carbon source availability, physical-chemical factors (temperature, pH and oxygen), agitation during growth process of microorganisms in culture medium and incubation time (Colla et al 2012, Kumar et al 2012, Veerapagu et al 2013.…”

Section: Resultsmentioning

confidence: 99%

Bioprospecting of lipolytic microorganisms obtained from industrial effluents

Peil

Kuss

Rave

et al. 2016

An. Acad. Bras. Ciênc.

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The lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. The aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in Pelotas, RS/Brazil. Bacteria were identified using Gram stain and biochemical tests (Vitek 2®). Fungi were identified according to macro and micromorphology characteristics. The extracellular lipase production was evaluated using the Rhodamine B test and the enzymatic activity by titration. Twenty-one bacteria were isolated and identified as Klebsiella pneumoniae ssp. pneumoniae, Serratia marcescens, Enterobacter aerogenes, Raoultella ornithinolytica and Raoultella planticola. Were characterized isolated filamentous fungi by the following genera: Alternaria sp., Fusarium sp., Geotrichum sp., Gliocladium sp., Mucor sp., Paecilomyces sp. and Trichoderma sp. Extracellular lipase production was observed in 71.43% of the bacteria and 57.14% of the fungi. The bacterium that presented better promising enzymatic activity was E. aerogenes (1.54 U/ml) however between fungi there was not significant difference between the four isolates. This study indicated that microorganisms lipase producers are present in the industrial effluents, as well as these enzymes have potential of biodegradation of lipid compounds.

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Section: Resultsmentioning

confidence: 99%

Bioprospecting of lipolytic microorganisms obtained from industrial effluents

Peil

Kuss

Rave

et al. 2016

An. Acad. Bras. Ciênc.

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“…Some lipases are capable of catalyzing the esterification, transesterification and interesterification of lipids (Burkert et al, 2004;Martins et al, 2008), water activity (a w ) being a determining factor in the reaction direction: forward (hydrolysis), equilibrium or reverse (synthesis) (Messias et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Lipases occur in plants, animals and microorganisms (Burkert et al, 2004;Martins et al, 2008;Roveda et al, 2010), but only microbial lipases can be produced in large-scale (Roveda et al, 2010;Qamsari et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…However, solid-state fermentation (SSF) has shown some advantages for enzyme production compared to SmF, even at a commercial scale (Thomas et al, 2013). It only needs a low percentage of free water to be conducted and it uses low cost substrates such as agricultural wastes, which act both as a source of nutrients for the fermentation process (Martins et al, 2008;Messias et al, 2011;Pandey, 2003), Martins et al, 2008). Among these microorganisms, filamentous fungi are considered the most suitable for processes involving SSF due to their ability to grow with a low amount of free water (Castiglioni et al, 2009;Thomas et al, 2013;Vendruscolo et al, 2007) together with their efficiency to degrade some pollutants (Rigas et al, 2007;Ye et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…There are several variables that influence lipase production by filamentous fungi, such as the source of carbon and nitrogen, the composition and pH of the culture medium, the fermentation temperature, and the geometry of the bioreactor (Colen et al, 2006;Martins et al, 2008). Therefore, this study is aimed to evaluate the effect of the composition of the culture medium on lipase production by Aspergillus niger grown in SSF using agro-industrial wastes supplemented with different carbon sources.…”

Section: Introductionmentioning

confidence: 99%

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LIPASE PRODUCTION BY Aspergillus niger GROWN IN DIFFERENT AGRO-INDUSTRIAL WASTES BY SOLID-STATE FERMENTATION

Costa

Hermann

García-Román

et al. 2017

Braz. J. Chem. Eng.

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-Filamentous fungi can easily degrade agro-industrial wastes in solid-state fermentation processes, synthesizing many important commercial biocompounds, such as lipolytic enzymes. The aim of this study was to evaluate the effect of the composition of the solid culture medium on the production of lipolytic enzymes by the fungus Aspergillus niger. Rice, wheat and soybean bran were mixed to prepare the culture medium, which was supplemented with glucose, glycerol or soybean oil. Four mixture experimental designs were used to find the best medium for enzyme production. According to our results, the highest lipolytic activity values were achieved with a mixture of rice bran and glycerol (19.844 U·g -1 ) or with rice bran only (13.267 U·g -1 ). Thus, the lipolytic enzyme could be produced without any additional carbon source apart from rice bran, although glycerol addition induced a higher production.

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Microbial biosurfactants as additives for food industries

Campos

Stamford

Sarubbo

et al. 2013

Biotechnology Progress

258

109

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Microbial biosurfactants with high ability to reduce surface and interfacial surface tension and conferring important properties such as emulsification, detergency, solubilization, lubrication and phase dispersion have a wide range of potential applications in many industries. Significant interest in these compounds has been demonstrated by environmental, bioremediation, oil, petroleum, food, beverage, cosmetic and pharmaceutical industries attracted by their low toxicity, biodegradability and sustainable production technologies. Despite having significant potentials associated with emulsion formation, stabilization, antiadhesive and antimicrobial activities, significantly less output and applications have been reported in food industry. This has been exacerbated by uneconomical or uncompetitive costing issues for their production when compared to plant or chemical counterparts. In this review, biosurfactants properties, present uses and potential future applications as food additives acting as thickening, emulsifying, dispersing or stabilising agents in addition to the use of sustainable economic processes utilising agro-industrial wastes as alternative substrates for their production are discussed.

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Co-produção de lipase e biossurfactante em estado sólido para utilização em biorremediação de óleos vegetais e hidrocarbonetos

Cited by 25 publications

References 17 publications

Bioprospecting of lipolytic microorganisms obtained from industrial effluents

Bioprospecting of lipolytic microorganisms obtained from industrial effluents

LIPASE PRODUCTION BY Aspergillus niger GROWN IN DIFFERENT AGRO-INDUSTRIAL WASTES BY SOLID-STATE FERMENTATION

Microbial biosurfactants as additives for food industries

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