Purificação e caracterização de uma gentioexaose obtida de botriosferana por hidrólise ácida parcial

Silva, Iara Ribeiro; Monteiro, Nilson K.; Martinez, Paula Felippe; Izeli, Nataly Lino; Vasconcelos, Ana Flora Dalberto; Cardoso, Marilsa de Stefani; Silva, Maria de Lourdes Corradi da; Barbosa-Dekker, Aneli M.; Dekker, Robert F. H.; Silva, Gil Valdo José da; Moraes, Luís A. Beraldo de

doi:10.1590/s0100-40422008000500013

Cited by 4 publications

(3 citation statements)

References 12 publications

(20 reference statements)

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“…Apesar disso, uma vantagem da hidrólise ácida está na possibilidade de ser realizada em temperaturas elevadas, promovendo maior fluidez das amostras submetidas a essa condição (SILVA et al, 2008).…”

Section: Obtenção De Oligossacarídeos Por Métodos Físicos E Químicosunclassified

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Produção, propriedades e aplicações de oligossacarídeos

et al. 2011

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ResumoOs oligossacarídeos participam da constituição da fibra alimentar e são principalmente utilizados como prebióticos. Esta revisão apresenta as vias de obtenção destes açúcares, os quais podem ser produzidos por síntese (química ou enzimática) ou da despolimerização de polissacarídeos (física, química ou enzimática), como descrito na presente revisão. Os oligossacarídeos vêm sendo utilizados comercialmente como ingredientes de cosméticos, medicamentos, produtos agrícolas e principalmente na indústria alimentícia. O potencial de aplicações dos oligossacarídeos nessas áreas nas mais diversas áreas tais como alimentos, rações animais, fármacos e cosméticos tem contribuído para o aumento das investigações científicas. O uso de oligossacarídeos como agentes imunomoduladores e modificadores de respostas biológicas foi recentemente descrito, assim como seus efeitos como compostos antiinflamatórios e hipocolesterolêmicos. Uma visão geral sobre as diversas funções nutracêuticas e biológicas destes oligômeros de carboidratos visando benefícios para a saúde humana também foi reportada. Palavras-chave: Oligossacarídeos. Fibras da dieta. Saúde humana. AbstractOligosaccharides participate in the formation of dietary fiber and are mainly used as prebiotic agents. This review presents ways of obtaining these sugars, which can be produced by synthesis (chemical or enzymatic), or through depolymerization of polysaccharides (physical, chemical or enzymatic). Oligosaccharides have also been used commercially as an ingredient in cosmetics, pharmaceuticals, agricultural products and especially in the food industry because of their physical properties. The potential applications of oligosaccharides in several areas such as food, animal feed, pharmaceuticals and cosmetics have contributed to the increase in scientific research on these carbohydrates. The use of oligosaccharides as immuno-modulatory agents and biological response modifiers has been recently described, and their effects as anti-inflammatory and in reducing cholesterol. An overview of the various nutraceutical and biological functions of these carbohydrates in order to benefit human health is also reported.

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Section: Obtenção De Oligossacarídeos Por Métodos Físicos E Químicosunclassified

“…A hidrólise foi realizada com a presença de ácido trifluoracético 0,2 M à 100° C durante 4 horas (SILVA et al, 2008).…”

Section: Obtenção De Oligossacarídeos Por Métodos Físicos E Químicosunclassified

Produção, propriedades e aplicações de oligossacarídeos

et al. 2011

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“…Polysaccharides are associated with polypeptides, constituting the cell wall glycoproteins, forming the external layers that are involved in different types of interactions with the extracellular environment. Several of these molecules are immunostimulatory compounds with a great potential, such as pathogenicity regulators and determinants for the host immune response (Silva et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response

Burjack

Santana-Filho

Ruthes

et al. 2014

Front. Cell. Infect. Microbiol.

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Dematiaceous fungi constitute a large and heterogeneous group, characterized by having a dark pigment, the dihydroxynaftalen melanin—DHN, inside their cell walls. In nature they are found mainly as soil microbiota or decomposing organic matter, and are spread in tropical and subtropical regions. The fungus Fonsecaea monophora causes chromoblastomycosis in humans, and possesses essential mechanisms that may enhance pathogenicity, proliferation and dissemination inside the host. Glycoconjugates confer important properties to these pathogenic microorganisms. In this work, structural characterization of glycan structures present in two different strains of F. monophora MMHC82 and FE5p4, from clinical and environmental origins, respectively, was performed. Each one were grown on Minimal Medium (MM) and Czapeck-Dox (CD) medium, and the water soluble cell wall glycoconjugates and exopolysaccharides (EPS) were evaluated by NMR, methylation and principal component analysis (PCA). By combining the methylation and 2D NMR analyses, it was possible to visualize the glycosidic profiles of the complex carbohydrate mixtures. Significant differences were observed in β-D-Galf-(1→5) and (1→6) linkages, α- and β-D-Glcp-(1→3), (1→4), and (1→6) units, as well as in α-D-Manp. PCA from 1H-NMR data showed that MMHC82 from CD medium showed a higher variation in the cell wall carbohydrates, mainly related to O-2 substituted β-D-Galf (δ 106.0/5.23 and δ 105.3/5.23) units. In order to investigate the antigenic response of the glycoconjugates, these were screened against serum from chromoblastomycosis patients. The antigen which contained the cell wall of MMHC82 grown in MM had β-D-Manp units that promoted higher antigenic response. The distribution of these fungal species in nature and the knowledge of how cell wall polysaccharides and glycoconjugates structure vary, may contribute to the better understanding and the elucidation of the pathology caused by this fungus.

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