A convenient method for lecithin purification from fresh eggs

Abstract

The increasing demand for fatty acid-free lecithin required modifications in existing purification methods. In this technical note we describe a purification procedure with the following steps: a) homogenization and extraction of yolks obtained from fresh eggs with acetone, b) solubilization with ethanol and solvent elimination and c) repeated solubilization/precipitation with petroleum ether/acetone. This crude extract was chromatographed on neutral alumina, which was exhaustively washed with chloroform before elution with chloroform:methanol, allowing the sequential separation of fatty acids and lecithin. Chromatographic behavior and mass spectra of the product are presented. This fast procedure yields fatty acid-free lecithin at a competitive cost

HPV16 Tumor Associated Macrophages Suppress Antitumor T Cell Responses

“…Egg phosphatidylcholine was prepared as described (29). Cholesterol (Sigma-Aldrich) was recrystallized from methanol/acetate.…”

Preparation of PVP hydrogel nanoparticles using lecithin vesicles

“…23 Soy lecithin was obtained from crude soy lecithin capsules by purification with the same method used for egg phosphatidilcholine. 23 Dioctadecyldimethylammonium chloride (DODAC) was obtained from the bromide salt (DODAB; Aldrich) after ion exchange with a Dowex-21K resin (Fluka) in the chloride form in methanol.…”

“…23 Soy lecithin was obtained from crude soy lecithin capsules by purification with the same method used for egg phosphatidilcholine. 23 Dioctadecyldimethylammonium chloride (DODAC) was obtained from the bromide salt (DODAB; Aldrich) after ion exchange with a Dowex-21K resin (Fluka) in the chloride form in methanol. 1,2-Dipalmitoyl-3-trimethylammonium-propane chloride (DPTMA) was synthesized as described previously 24 as bromide salt, which was exchanged for chloride as indicated for DODAC.…”

“…Lecithins of different sources exhibit distinct properties. 23,29 In particular the length of the apolar portion of the molecule and the insaturation of the alkyl chain will result in vesicles with different properties. 30 The method used to obtain vesicle encapsulated PVP also contributes to high encapsulation efficiency.…”

Isolation and Purification of Phosphatidylcholine from Egg Yolk by Semipreparative Hydrophilic Interaction Liquid Chromatography

“…Additionally, column chromatography, semipermeable membrane, precipitation with inorganic salt, as well as supercritical fluid extraction (SFE) are also used for isolation of PC. [4] In column chromatography, alumina, silica, and manganese dioxide are preferred packing materials. [5][6][7] The eluants are usually toxic chloroform and hexane, which lead to adverse impact on the environment and human health.…”