Avaliação da estabilidade do marcador plasmático do estresse oxidativo: malondialdeído

Abstract

Recebido em 15/1/07; aceito em 2/8/07; publicado na web em 19/12/07 EVALUATION OF THE STABILITY OF THE OXIDATIVE STRESS PLASMATIC BIOMARKER-MALONDIALDEHYDE. Malondialdehyde (MDA) is one of the lipid peroxidation products widely used as indicator of cellular injury. However, the short-term and the long-term stability of this biomarker remain unclear. The objective of this work was to evaluate the stability of plasmatic MDA at-20 o C, utilizing thiobarbituric acid (TBA) as derivative in spectrophotometric and chromatographic analysis. The results showed that MDA was stable for 24 h after blood collection, was not stable when stored after alkaline hydrolysis, remained stable for 30 days after TBA derivatization and was stable for 3 days when stored after n-butanol extraction, all at-20 o C.

Longitudinal Study of Delta-Aminolevulinate Dehydratase Activity and Oxidative Profile in Healthy Pregnant Women

“…Previous studies have reported conflicting results pertaining to MDA levels in pregnancy, Yüksel et al [38] verified a decrease in MDA levels in the third trimester, whereas in contrast, Bukhari et al [31] demonstrated an increase through pregnancy. The produced MDA can be quantified through the TBARS technique as it reacts easily with thiobarbituric acid (TBA) [37,39]. In spite of that, in the present study, increased TBARS levels in plasma (Figure 2A) and erythrocytes (Figure 2B) were observed in the second trimester when compared to the other two gestational trimesters.…”

“…The biochemical events caused by the action of ROS on the unsaturated lipids of cell membranes can be characterized by lipid peroxidation. One of the main markers of oxidative stress for assessing lipid damage is malondialdehyde (MDA), which is formed through the oxidation of polyunsaturated fatty acids, mainly arachidonic acid [13,17,36,37]. Previous studies have reported conflicting results pertaining to MDA levels in pregnancy, Yüksel et al [38] verified a decrease in MDA levels in the third trimester, whereas in contrast, Bukhari et al [31] demonstrated an increase through pregnancy.…”

An experimental model of contact dermatitis: Evaluation of the oxidative profile of Wistar rats treated with free and nanoencapsulated clobetasol

“…Levels of MDA are positively related to lipid peroxidation and it is a commonly used biomarker. 43,44 Sensitization with NiSO 4 resulted in damage to the liver tissue as indicated by an increased level of MDA. This also resulted in an increase in NPSH and in the antioxidant activity of catalase.…”

Antioxidantes e sua relação com os radicais livres, e Doenças Crônicas Não Transmissíveis: uma revisão de literatura / Antioxidants and their relationship with free radicals, and Chronic Non communicable Diseases: a literature review

“…Diversos biomarcadores vem sendo usados para avaliar o estresse oxidativo, entre eles encontra-se o malondialdeído (MDA), que é um dos mais aplicados por tratar-se de um dos produtos secundários produzidos e conhecidos da peroxidação lipídica. Porém, os biomarcadores do estresse oxidativo costumam ser inconstantes e sendo assim, as análises válidas dos biomarcadores nas amostras biológicas são importantes, sendo que falsos resultados podem ser obtidos se as análises não acontecerem sob as condições adequadas (GROTTO et al, 2008).…”