A simple and rapid method for urinary acetone analysis by headspace/gas chromatography

Abstract

Recebido em 31/5/06; aceito em 9/11/06; publicado na web em 6/8/07Urinalysis of acetone is important to monitor workers occupationally exposed to acetone and/or isopropanol, as well as in diagnosis of some diseases related to lipid metabolism impairment. This work shows a sensitive, simple and rapid static headspace-gas chromatographic procedure for quantitative determination of acetone in urine. The method was applied to measure acetone in 207 samples from general population volunteers, resulting in a mean level of 1.12 mg/L (± 0.47) and a range of 0.20 -1.95 mg/L. The method is reproducible and reliable, making it suitable for routine analysis of acetone in urine.

Sniffing Out Urinary Tract Infection—Diagnosis Based on Volatile Organic Compounds and Smell Profile

“…If headspace analysis is to be performed, the temperature of the sample is commonly elevated, to increase the sample vapour pressure [ 143 ]. The duration and temperatures for this procedure vary across studies, e.g., 5–20 min and 37–90 °C [ 42 , 144 , 145 , 146 ]. Lastly, while urine constitutes a highly complex and rich source of analytes, headspace gas samples are unlikely to represent the full metabolite content of the medium.…”

Capped ZnO quantum dots with a tunable photoluminescence for acetone detection

“…17 Several techniques have been used to detect acetone concentration, such as FTIR spectroscopy, chromatography, and chemical method, but these methods are expensive and complex. 15,18,19 Therefore, the need for a less risky, lowcostly, and high-performance method, which may sense the low concentration of CH 3 COCH 3 levels (ppb), is urgent to diagnose people with diabetics early in humans. 14 Metal oxide semiconductors have been evaluated as candidates amongst various active sensing matters utilized for detecting acetone because of their remarkably high sensitivity, ultrahigh porosity, and extraordinarily high surface area.…”

“…APTES is used as an effective new capping agent in synthesis quantum dots to improve sensitivity and optical properties besides the most stabilization for QDs and also detecting acetone more than that reported in literature. 15,18,19 This work uses the sol-gel method to prepare pristine ZnO QDs and APTES/ZnO QDs. Analytical techniques such as FT-IR, XPS, EDX, XRD, HRTEM, zeta potential, UV-visible, and PL spectra are used to discuss the morphological, structural, surface properties and optical characteristics of these QDs.…”

Strategies in Improving Sensitivity of Colorimetry Sensor Based on Silver Nanoparticles in Chemical and Biological Samples

“…De Oliveira and de Sequira [25] monitored acetone levels in 207 workers' urine exposed to acetone/ isopropanol at work and the detection of diseases related to lipid metabolism disorders. Quantitative analysis was performed using the headspace/gas chromatography method.…”

“…Quantitative analysis was performed using the headspace/gas chromatography method. The result showed that the mean acetone level was 1.12 ± 0.47 mg/L with a measurement range of 0.20-1.95 mg/L [25]. Teerlink [26] analyzed arginine residues, dimethylarginine asymmetry (ADMA), which is a nitric oxide synthase inhibitor and is suspected to be a signal of endothelial dysfunction in cardiovascular disease using the High-Performance Liquid Chromatography (HPLC) method.…”