Quimioluminiscencia electrogenerada del luminol usando electrodos de bajo costo

Salgado, Guillermo; Navarrete, José; Bustos, Carlos; Sánchez, C Gonzalo; Ugarte, Ricardo

doi:10.1590/s0100-40422006000200033

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…In alkaline medium, luminol is electrochemically oxidized to diazaquinone at 0.5 V on the screen-printed graphite electrode and reacts with hydrogen peroxide produced by atmospheric lactate oxidation catalyzed by LOx giving a luminescent emission. 21,53 If the medium is more alkaline, a higher emission is obtained, due to an increase in luminol deprotonation, 46,54,55 as shown in Fig. 5A.…”

Section: Ecl Mechanismmentioning

confidence: 94%

Disposable electrochemiluminescent biosensor for lactate determination in saliva

Ballesta-Claver

Miron

Capitán-Vallvey

2009

Analyst

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An electrochemiluminescence-based disposable biosensor for lactate is characterized. The lactate recognition system is based on lactate oxidase (LOx) and the transduction system consists of luminol. All the needed reagents, luminol, LOx, BSA, electrolyte and buffer have been immobilized by a Methocel membrane placed on the working electrode of the screen-printed electrochemical cell. The measurement of the electrochemiluminescence (ECL) is made possible via a photocounting head when 50 microl of sample is placed into the screen-printed cell with a circular container containing the disposable sensing membrane. The compositions of the membrane and reaction conditions have been optimized to obtain adequate sensitivity. The disposable biosensor responds to lactate after 20 s when two 1 s pulses at 0.5 V are applied to obtain the analytical parameter, the ECL initial rate. The linearized double logarithmic dependence for lactate shows a dynamic range from 10(-5) to 5 x 10(-4) M with a detection limit of 5 x 10(-6) M and a sensor-to-sensor repeatability, as relative standard deviation, RSD, of 3.30% at the medium level of the range. The ECL disposable biosensor was applied to the analysis of lactate in human saliva as an alternative procedure for obtaining the lactate level in a non-invasive way. Interferences coming from components of saliva were studied and eliminated in a simple way that was easy to handle. The procedure was validated for use in human saliva, comparing the results against an enzymatic reference procedure. The proposed method is quick, inexpensive, selective and sensitive and uses conventional ECL instrumentation.

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Section: Ecl Mechanismmentioning

confidence: 94%

Disposable electrochemiluminescent biosensor for lactate determination in saliva

Ballesta-Claver

Miron

Capitán-Vallvey

2009

Analyst

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“…If the medium is more alkaline, a higher emission is obtained, due to an increase in luminol deprotonation [ 30 - 32 ]. In the absence of lactate, a background ECL signal is observed that starts to grow from pH 9.0 up to 10.5 (the background ECL signal increases 206.3 V/s per pH unit).…”

Section: Resultsmentioning

confidence: 99%

A Portable Luminometer with a Disposable Electrochemiluminescent Biosensor for Lactate Determination

Martínez-Olmos¹,

Ballesta-Claver

Palma³

et al. 2009

Sensors

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A hand-held luminometer for measuring electrochemiluminescence (ECL) for lactate determination and based on one-shot biosensors fabricated using screen-printed electrodes is described. The lactate recognition system is based on lactate oxidase and the transduction system consists of electro-oxidation of luminol, with all the reagents immobilized in a Methocel membrane. The membrane composition and reaction conditions have been optimized to obtain adequate sensitivity. The luminometer is based on a large silicon photodiode as detector and includes a programmable potentiostat to initialize the chemical reaction and signal processing circuitry, designed to acquire a low level photocurrent with offset cancelation, low pass filtering for noise attenuation and adjustable gain up to 1012 V/A. The one-shot biosensor responds to lactate rapidly, with an acquisition time of 2.5 min, obtaining a linear dependence from 8 × 10−6 to 2 × 10−4 M, a detection limit of 2.4 × 10−6 M and a sensor-to-sensor reproducibility (relative standard deviation, RSD) of around 7–10 % at the medium level of the range.

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Quimioluminiscencia electrogenerada del luminol usando electrodos de bajo costo

Cited by 2 publications

References 15 publications

Disposable electrochemiluminescent biosensor for lactate determination in saliva

Disposable electrochemiluminescent biosensor for lactate determination in saliva

A Portable Luminometer with a Disposable Electrochemiluminescent Biosensor for Lactate Determination

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