Isolamento do alcalóide ricinina das folhas de Ricinus communis (Euphorbiaceae) através de cromatografias em contracorrente

Leite, Ana Cristina Lima; Cabral, Elaine C.; Santos, Djalma A. P. dos; Fernandes, João B.; Vieira, Paulo C.; Silva, M. Fátima das G. F. da

doi:10.1590/s0100-40422005000600009

Cited by 21 publications

(11 citation statements)

References 17 publications

(24 reference statements)

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“…The larvicidal activity of the R. communis oil can be attributed to its chemical components which include the a derivative of ricin ricinoleic acid, a toxic substance that is present in abundance in the seed oil of this plant 7 . This constituent had a negative effect on the larval of this vector.…”

Section: Discussionmentioning

confidence: 99%

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Bioactivity of plant extracts on the larval and pupal stages of Aedes aegypti (Diptera, Culicidea)

Candido

Cavalcanti

Beserra

2013

Rev. Soc. Bras. Med. Trop.

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Introduction: Aedes aegypti is responsible for the transmission of the dengue and yellow fever viruses. This study evaluated the effects of extracts from Cnidosculos phyllacanthus, Ricinus communis, and Coutarea hexandra on the developmental periods of A.aegypti larvae and pupae. Crude extracts of C. phyllacanthus and C. hexandra and oil from R. communis and C. phyllacanthus were used. Methods: Bioassays of the larvicidal and pupicidal effects of these products at different concentrations and times of exposure were evaluated. The lethal and sublethal effects were determined using different concentrations in larvicidal tests. Mortality data were evaluated by Probit analysis to determine the LC 50 and LC 90 values. Results: The vegetable oils from C. phyllacanthus and R. communis demonstrated greater effi ciency for larval control with an LC 50 =0.28µl/mL and an LC 90 =1.48µl/ mL and LC 50 =0.029µl/mL and a LC 90 =0.26µl/mL, respectively. In pupal tests toxic effects for all insects were verifi ed after exposure to the products at signifi cant LC 50 and LC 90 values for 24 and 48h. The effects of sublethal concentrations of C. phyllacanthus (oil) were more effective on the insects. Conclusions: The vegetables oils from C. phyllacanthus and R. communis demonstrated greater potential from the control of different developmental periods in the life cycle of this insect.

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Section: Discussionmentioning

confidence: 99%

“…Thus, Cnidoscolus phyllacanthus, Ricinus communis (Euphorbiaceae) and Coutarea hexandra (Rubiaceae) are chemical compounds whose biological activities have been described [5][6][7][8] .…”

mentioning

confidence: 99%

Bioactivity of plant extracts on the larval and pupal stages of Aedes aegypti (Diptera, Culicidea)

Candido

Cavalcanti

Beserra

2013

Rev. Soc. Bras. Med. Trop.

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“…O isolamento de ricinina a partir do extrato diclorometânico das folhas foi realizado através de cromatografia contracorrente de alta velocidade (HSCCC) a partir de 1,5 g do extrato bruto conforme LEITE et al (2005). A ricinina foi obtida na forma pura nas frações 35-49 (209 mg).…”

Section: Methodsunclassified

Suscetibilidade de operárias e larvas de abelhas sociais em relação à ricinina

Rother

Souza

Malaspina

et al. 2009

Iheringia, Sér. Zool.

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ABSTRACT. Susceptibility of workers and larvae of social bees in relation to ricinine. Many substances of vegetal origin can be toxic or present an insecticidal potential. With the aim of decreasing the environment pollution problem, a few studies are trying to substitute synthetic insecticides with botanical ones. Ricinus communis (Euphorbiaceae) presents a great variety of substances, being the ricinine the main toxic component. Considering that bees are useful as pollinator agents of plants, this study evaluates toxicity potential of ricinine on workers and larvae of Apis mellifera (Linnaeus, 1758) (Hymenoptera, Apidae) and Scaptotrigona postica (Latreille, 1907) (Hymenoptera, Meliponini). In order to determine ricinine toxicity, ingestion tests were carried out with isolated workers bees that received ricinine on its diet. Furthermore, for topic tests, solutions of ricinine in methanol were applied on pronotum of worker bees with an "Agla" brand micrometer syringe outfit. For larvae of bees, ingestion tests were used and mortality rates were calculated. According to the results, it was detected a significant toxic activity (p < 0.0001) of ricinine on the workers of the two bee species at concentrations of 0.1% in the ingestion tests and 0.2% in the topic tests. For all concentrations (0.25%, 0.5% and 1%), a high percentage of larvae bees was negatively affected by ricinine on the first days of life. KEYWORDS. Ricinus communis, Apis mellifera, Scaptotrigona postica, topic test, ingestion test.RESUMO. Muitas substâncias de origem vegetal podem ser tóxicas ou apresentar potencial inseticida. Com o objetivo de diminuir a problemática da poluição ambiental alguns estudos vêm tentando substituir os inseticidas artificiais pelos inseticidas botânicos. Ricinus communis (Euphorbiaceae) apresenta uma grande variedade de substâncias sendo a ricinina o principal componente tóxico. Considerando que as abelhas são insetos benéficos por atuarem como agentes polinizadores das plantas, este estudo teve por objetivo avaliar o efeito tóxico da ricinina para as operárias e larvas de Apis mellifera (Linnaeus, 1758) (Hymenoptera, Apidae) e Scaptotrigona postica (Latreille, 1907) (Hymenoptera, Meliponini). Para isso, foram realizados testes de ingestão em operárias confinadas recebendo ricinina incorporada à dieta e testes de aplicação tópica com a substância solubilizada em metanol e aplicada no pronoto das abelhas com auxílio de uma microseringa. Para as larvas foram realizados testes de ingestão e calculada sua taxa de mortalidade. Os resultados mostram atividade tóxica significativa (p < 0.0001) da ricinina nas abelhas adultas das duas espécies para a concentração 0,1% nos testes de ingestão e 0,2% nos testes de aplicação tópica. Outrossim, uma elevada porcentagem de larvas foi afetada negativamente pela ricinina logo nos primeiros dias de vida para todas as concentrações testadas (0,25%, 0,5% e 1%). PALAVRAS-CHAVE. Ricinus communis, Apis mellifera, Scaptotrigona postica, teste de aplicação tópica, teste de ingestão.Apesar d...

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“…It is especially suitable for separation and purification of active components from natural products. [18][19][20] However, there are no reports of using SFE to extract and HSCCC to isolate chemical compounds from D. dasycarpus. The aim of this paper was to develop an efficient method for the preparative extraction, separation and purification of dictamnine, obacunone and fraxinellone with high purity from D. dasycarpus by SFE and HSCCC.…”

Section: Introductionmentioning

confidence: 99%

Extraction and isolation of dictamnine, obacunone and fraxinellone from Dictamnus dasycarpus Turcz. by supercritical fluid extraction and high-speed counter-current chromatography

Yang¹,

Lin²,

Geng³

et al. 2012

Quím. Nova

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Recebido em 19/4/11; aceito em 10/8/11; publicado na web em 30/9/11 Supercritical fluid extraction was used to extract active compounds from the Chinese traditional medicinal D. dasycarpus under the pressure of 30 MPa and temperature of 45 ºC. Further separation and purification was established by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.8:1.3:0.9, volume ratio). The separation yielded a total of 47 mg of dictamnine, 24 mg of obacunone and 83 mg of fraxinellone from 1.0 g of the crude extract in one step separation with the purity of 99.2, 98.4 and 99.0%, respectively, as determined by HPLC. The chemical structures of these compounds were identified by ESI-MS, IR, 1 H-NMR and 13 C-NMR.

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Isolamento do alcalóide ricinina das folhas de Ricinus communis (Euphorbiaceae) através de cromatografias em contracorrente

Cited by 21 publications

References 17 publications

Bioactivity of plant extracts on the larval and pupal stages of Aedes aegypti (Diptera, Culicidea)

Bioactivity of plant extracts on the larval and pupal stages of Aedes aegypti (Diptera, Culicidea)

Suscetibilidade de operárias e larvas de abelhas sociais em relação à ricinina

Extraction and isolation of dictamnine, obacunone and fraxinellone from Dictamnus dasycarpus Turcz. by supercritical fluid extraction and high-speed counter-current chromatography

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