Drosophila melanogaster Meigen: 3. sensibilidade ao carbofuran e biomonitoramento de seus resíduos em repolho

Abstract

Recebido em 11/9/00; aceito em 23/3/01 DROSOPHILA MELANOGASTER MEIGEN: 3. SUSCEPTIBILITY TO CARBOFURAN AND BIOMONITORING OF ITS RESIDUES IN CABBAGE. The susceptibility of Drosophila melanogaster to carbofuran and the use of this organism in biomonitoring residues of the insecticide in cabbage was evaluated. Under the conditions of the bioassay, residues-film bioassay in Petri dish, carbofuran degraded depending on the temperature and time of exposure. Bioassays conducted with D. melanogaster showed that its toxicity increases with temperature (20 to 35 °C). LC 50 values, calculated as a function of temperature, ranged from 3.6 to 10.5 mg/g body weight (bw) for males and from 2.9 to 8.7 mg/g bw for females. The formulated product Furadan ® G was applied on cabbage (Brassica oleracea, var. capitata) and the residues of carbofuran were determined by bioassay. The determination limit of the bioassay was 0.1 mg/kg and the method presented reproducibility with coefficient variation of 17 %. The validation of the bioassay by high performance liquid chromatography confirms the viability of the bioassay with D. melanogaster in monitoring the residues of carbofuran in cabbage.