Aumento da resolução de espectros de RMN 13C de ácidos húmicos em solução através do tratamento prévio com KCl 0,03 mol L-1

Canellas, Luciano Pasqualoto; Guridi, Fernando; Santos, Gabriel de Araújo; Rumjanek, Víctor Marcos; Braz‐Filho, Raimundo

doi:10.1590/s0100-40422001000100021

Quím. Nova

2001

DOI: 10.1590/s0100-40422001000100021

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Aumento da resolução de espectros de RMN 13C de ácidos húmicos em solução através do tratamento prévio com KCl 0,03 mol L-1

Luciano Pasqualoto Canellas¹,

Fernando Guridi²,

Gabriel de Araújo Santos³

et al.

Abstract: Recebido em 7/10/99; aceito em 28/2/00 INCREASE RESOLUTION OF SOLUTION 13 C NMR SPECTRA OF HUMIC ACIDS BY PREVIOUS TREATMENT WITH 0,03 MOL L-1 KCl. High levels of Fe and Mn present in some soils and compost organic matter decrease the resolution of 13 C NMR spectra of humic substances. Addition of KCl up to a concentration of 0,03 mol L-1 to humic substances extracts followed by centrifugation is an efficient method of eliminating clays and minerals containing high levels of paramagnetic metals such as Fe and … Show more

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Cited by 2 publications

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Bioatividade de ácidos húmicos: efeitos sobre o desenvolvimento radicular e sobre a bomba de prótons da membrana plasmática

Façanha

Okorokova‐Façanha

Olivares

et al. 2002

Pesq. agropec. bras.

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Resumo -A bioatividade de ácidos húmicos (AH) isolados de lodo da estação de tratamento de esgoto (AHL) e de vermicomposto (AHV) foi avaliada pela ação dessas substâncias sobre o transporte de prótons através da membrana plasmática de células de raízes de café e milho e sua relação com o desenvolvimento dessas espécies. Houve estímulo da área superficial radicular em ambas as espécies cultivadas com ambos AH, mostrando uma concentração ótima em torno de 40 mg L -1 . Nessa condição, os tratamentos com AHL e AHV estimularam a H + -ATPase de membrana plasmática em plântulas de café e milho. Os AHL foram mais efetivos na promoção desses efeitos do que os AHV. A modificação do perfil cromatográfico dos AH em solução antes e após o cultivo das plântulas revelou que a interação planta-AH promoveu uma redistribuição das massas moleculares dessas substâncias, sugerindo uma dinâmica de mobilização de subunidades funcionais dos AH por exsudatos das raízes. A análise estrutural dos AH detectou a presença de grupamentos de auxina. A análise comparativa da ação desses dois AH sobre as espécies representantes de plantas monocotiledôneas (milho) e dicotiledôneas (café) apontam para a ativação da H + -ATPase de plasmalema como possível marcador metabólico de bioatividade dos ácidos húmicos.Termos para indexação: Zea mays, Coffea arabica, lodo residual, ácidos orgânicos, vermicomposto. Humic acids bioactivity: effects on root development and on the plasma membrane proton pumpAbstract -The bioactivity of humic acids (HA) isolated from sludge of the station of sewer treatment (HAL) and from vermicompost (HAV) was evaluated through the action of those substances on primary transport of protons of the plasma membrane of coffee and corn root cells and its relationship with the development of those species. A stimulation in the superficial area of roots was observed for both species cultivated with both humic acids, exhibiting an optimum concentration, about 40 mg L -1 of HA. In this condition the treatment with HAL and HAV stimulated the plasma membrane H + -ATPase of corn and coffee roots. HAL were more effective to promote those effects than HAV. The modification of the chromatographic profile of the HA in solution before and after the cultivation of the seedlings revealed that the interaction plant-HA promoted a rearrangement of the average molecular weight of those substances suggesting a dynamic mobilization of bioactive subunits of the HA by plant exudates. The structural analysis of the HA has detected the presence of auxin groups. A comparative analysis of the action of those HA on the monocotyledonous (corn) and dicotyledonous (coffee) plants indicates to the activation of plasmallema H + -ATPase as a possible metabolic marker for bioactivity of humic acids.

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Bioatividade de ácidos húmicos: efeitos sobre o desenvolvimento radicular e sobre a bomba de prótons da membrana plasmática

Façanha

Okorokova‐Façanha

Olivares

et al. 2002

Pesq. agropec. bras.

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Biostimulation of inoculation with Glomus proliferum and application of humic acid in the in vitro growth of Lunularia cruciata

et al. 2013

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In this study, we evaluated the growth of the liverwort Lunularia cruciata, inoculated or not with the arbuscular mycorrhizal fungi (AMF) Glomus proliferum (15 spores per Petri dish), in Strullu-Romand Variant (SRV) medium modified and enriched with humic acid (HA) at different concentrations (0, 20, 40 and 80 mg C L −1 ), as well as the sporulation of the AMF. We assessed the absolute growth rate (AGR) and relative growth rate (RGR) at inoculation, as well as at 7, 14, 21, 28, 43, 52 and 60 days after inoculation (DAI), whereas we assessed sporulation at 25, 43, 60 and 70 DAI. The main determinant of L. cruciata growth was the presence of AMF. With and without G. proliferum inoculation, respectively, the AGR peaked at 39 and 42 DAI, and the RGR was 0.0474 and 0.0387 cm 2 cm −2 d −1 . Doses of 20 and 80 mg C L −1 of HA had a positive influence on the growth of L. cruciata. With and without HA, respectively, the AGR peaked at 38 and 39 DAI, and the RGR was 0.0484 and 0.0422 cm 2 cm −2 d −1 . The sporulation of G. proliferum, which was as high as 199 spores plate −1 , was influenced by HA, especially at 20 and 80 mg C L −1 .

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Aumento da resolução de espectros de RMN 13C de ácidos húmicos em solução através do tratamento prévio com KCl 0,03 mol L-1

Cited by 2 publications

References 10 publications

Bioatividade de ácidos húmicos: efeitos sobre o desenvolvimento radicular e sobre a bomba de prótons da membrana plasmática

Bioatividade de ácidos húmicos: efeitos sobre o desenvolvimento radicular e sobre a bomba de prótons da membrana plasmática

Biostimulation of inoculation with Glomus proliferum and application of humic acid in the in vitro growth of Lunularia cruciata

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