Microcalorimetria: uma técnica aplicável ao estudo do diauxismo da Saccharomyces cerevisiae

Rettori, Daniel; Volpe, Pedro L. O.

doi:10.1590/s0100-40422000000200018

Cited by 7 publications

(6 citation statements)

References 1 publication

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“…At a 0.09 to 0.9% glucose concentration in culture medium, S. cerevisiae glucose catabolism chooses preferably the anaerobic biochemical way, even in the presence of O 2 , leading to glucose repression, due to gene repression which codifies the Krebs cycle of respiratory chain (Rettori and Volpe, 2000). A previous work (Montenegro 1999) found in S. cerevisiae cultures exposed to 220mT SMF an increased (72%) production of carbonic gas in relation to the control groups.…”

Section: Introductionmentioning

confidence: 86%

Influence of static magnetic fields on S. cerevisae biomass growth

Muniz

Marcelino

Schuler

et al. 2007

Braz. arch. biol. technol.

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Biomass growth of Saccharomyces cerevisiae DAUFPE-1012 was studied in eight batch fermentations exposed to steady magnetic fields (SMF) running at 23ºC (± 1ºC), for 24 h in a double cylindrical tube reactor with synchronic agitation. For every batch, one tube was exposed to 220mT flow intensity SMF, produced by NdFeB rod magnets attached diametrically opposed (N to S) magnets on one tube. In the other tube, without magnets, the fermentation occurred in the same conditions. The biomass growth in culture (yeast extract + glucose 2%) was monitored byspectrometry to obtain the absorbance and later, the corresponding cell dry weight. The culture glucose concentration was monitored every two hours so as the pH, which was maintained between 4 and 5. As a result, the biomass (g/L) increment was 2.5 times greater in magnetized cultures (n=8) as compared with SMF non-exposed cultures (n=8). The differential (SMF-control) biomass growth rate (135%) was slightly higher than the glucose consumption rate (130 %) leading to increased biomass production of the magnetized cells

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Section: Introductionmentioning

confidence: 86%

Influence of static magnetic fields on S. cerevisae biomass growth

Muniz

Marcelino

Schuler

et al. 2007

Braz. arch. biol. technol.

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“…The bioprocess involved can be correlated with the thermal effects generated by the bioactivity in a given system. [12][13] Thus, the increasing application of calorimetry caused an enormous positive impact, of fundamental importance, on the investigation of small insect metabolism, 14 and cellular 15 and soil microbial activities. 12 In each case, the evolution of a thermal effect, which is directly correlated with the microbial biomass, molecular oxygen consumption and carbon gas evolution by respiration of the microbiota, can be quantitatively determined.…”

Section: Introductionmentioning

confidence: 99%

Toxic effect caused on microflora of soil by pesticide picloram application

Prado

Airoldi

2001

J. Environ. Monitor.

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The toxic effect of the herbicide picloram on the microbial population of a typical Brazilian red Latosol soil was studied in a series of microcalorimetric experiments. The activity of the soil was stimulated by the addition of 6.0 mg of glucose and 6.0 mg of ammonium sulfate, under 34.8% controlled moisture, to a 1.50 mg soil sample, at 298.15 +/- 0.02 K. The net thermal effect due to the addition of the picloram to the soil was determined by interpreting the power-time curves, which were recorded on the microcalorimeter. The total thermal effect evolved by the microorganisms was affected by the increasing doses of herbicide, and varied from 0 to 10.00 micrograms g-1. An increase in picloram exposure caused a decrease of the original thermal effect, reaching a null value above 20.89 micrograms of herbicide per gram of soil. The decreases of the thermal effect evolved by microorganisms and the increase of the lag phase period are associated with the death of the microbial population. The effects caused by picloram application in this typical Brazilian soil resulted in a strong effect on the soil microbial communities.

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“…Segunda fase: as moléculas de gliceraldeido-3-fosfato são utilizadas para a produção de energia através da produção de ATP e piruvato. Após a produção do piruvato, condições de anaerobiose ou aerobiose irão ser cruciais para os produtos finais destas reações [9].…”

Section: Figuraunclassified

Fermentação alcoólica: proposta do ensino das vias bioquímicas através da destilação fracionada

2016

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Teaching of biology and chemistry in the schools has been widely discussed. The use of demonstration classes to content assimilation explained in lectures has been rarely used; hampering a better understanding of the students explained the content in the classroom. This article presents a proposal in science education combining theory and practice to facilitate the capture knowledge by students. The use of a simple distillation system for the explanation of metabolic pathways is a methodology that may present improvements in learning.

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Microcalorimetria: uma técnica aplicável ao estudo do diauxismo da Saccharomyces cerevisiae

Cited by 7 publications

References 1 publication

Influence of static magnetic fields on S. cerevisae biomass growth

Influence of static magnetic fields on S. cerevisae biomass growth

Toxic effect caused on microflora of soil by pesticide picloram application

Fermentação alcoólica: proposta do ensino das vias bioquímicas através da destilação fracionada

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