Metodologias gerais empregadas no isolamento e identificação estrutural de feromônios de insetos

Zarbin, Paulo H. G.; Ferreira, J. J.; Leal, Walter S.

doi:10.1590/s0100-40421999000200018

Cited by 32 publications

(13 citation statements)

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Section: Methodsmentioning

confidence: 99%

“…The columns were washed with bidistilled hexane, and the extracts were concentrated to 100 µL, under argon stream, in a clean conical bottom vial. 25 Gas chromatographic analyses were performed on a Varian 3800 GC equipped with FID, electronic pressure control, and operated in the splitless mode. A DB-5 (30 mm × 0.25 mm × 0.25µm) capillary column was used under the following analytical conditions: initial temperature of 70 ºC for 1 min, with an increase of 7 o C min -1 until a final temperature of 250 ºC, which was maintained for 10 min.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of porous silica glasses as insect pheromone dispensers

Tiboni

Coracini

Lima

et al. 2008

J. Braz. Chem. Soc.

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Este trabalho demonstra a viabilidade da utilização de vidros porosos como materiais para armazenar e liberar feromônios de insetos, visando o controle de pragas na agricultura. Feromônios de diferentes insetos foram impregnados em vidros porosos do tipo Vycor (PVG), e o material impregnado foi caracterizado por espectroscopia de absorção no infravermelho. A determinação da taxa de liberação do feromônio em laboratório, bem como o teste prático de captura de insetos em diferentes plantações, foram subseqüentemente realizados. Foram realizados testes com feromônios da Grapholita molesta (Lepidoptera: Tortricidae) e Leucoptera coffeella (Lepidoptera: Lyonetiidae), as principais pestes da maçã e café, respectivamente, em plantações brasileiras. Verificou-se que a taxa de liberação do feromônio depende do tipo de interação com as paredes dos poros do PVG. Os resultados indicaram uma boa performance do PVG, semelhante à de septos de borracha utilizados comercialmente como liberadores. Estes resultados, somados ao fato de que o PVG não é nocivo ao meio ambiente e pode ser reutilizado em vários ciclos de impregnação e liberação, fazem destes vidros porosos candidatos em potencial para aplicação no controle de pragas na agricultura.This work demonstrates the viability and advantages of using porous silica glass as an insect pheromone dispenser, in order to control insect population in agriculture. Pieces of so-called Porous Vycor Glass (PVG) were impregnated with pheromones of different insects (both consisting of a single compound or a mixture of compounds). Studies were conducted to characterize the impregnated-PVG and to determine the pheromone release ratio under laboratory conditions. The practical application was verified in two different plantation areas, using the PVG impregnated with pheromone from Grapholita molesta (Lepidoptera: Tortricidae) and Leucoptera coffeella (Lepidoptera: Lyonetiidae), the main pests of apple and coffee plantations in Brazil, respectively. The release rate was dependent on the interaction between the molecules of the impregnated pheromone and the surface of the PVG pores. A good performance of the porous glass was observed, similar to that of rubber septa commercially used as pheromone dispensers.Keywords: porous glass, adsorption, pheromones, slow release, chemical ecology IntroductionThe number of insects and other species that are developing resistance to pesticides is growing steadily, forcing chemical companies to work out novel pesticide formulas. The concept of integrated pest management (IPM) was developed in response to the problems caused by the increased use of conventional pesticides. IPM combines chemical, biological, and agrotechnical approaches to achieve pest control at a reasonable cost, while minimizing damage to the environment. The first step in IPM is to perform effective monitoring through the uses of pheromones. [1][2][3] Pheromones are substances which occur in nature and are used for chemical communication between animals of the same species. The main ways by ...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Evaluation of porous silica glasses as insect pheromone dispensers

Tiboni

Coracini

Lima

et al. 2008

J. Braz. Chem. Soc.

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“…8, 2016 Collection of volatiles Volatiles were collected by aeration. 10 Ten males and ten females were placed separately in aeration chambers (33 × 3.5 cm ID), and a continuous flow of 1 L min -1 of humidified and charcoal-filtered air was pulled through each chamber, carrying the volatiles to glass tubes (6 × 0.3 cm) containing 20 mg HayeSep ® (80/100 mesh, Sigma-Aldrich, Germany). After 24 h, the volatiles were eluted from the polymer with hexane (2 × 300 µL) and concentrated to 100 µL (10 µL per insect) under a gentle flow of argon.…”

Section: Insectsmentioning

confidence: 99%

Identification and Synthesis of the Male-Produced Sex Pheromone of the Soldier BeetleChauliognathus fallax(Coleoptera: Cantharidae)

Vidal

Fávaro

Guimarães

et al. 2016

Journal of the Brazilian Chemical Society

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“…4 A humidified charcoalfiltered airstream (1 L min -1 ) was maintained through the aeration apparatus. The columns were washed with distilled hexane, and the extracts were concentrated to 1 insect equivalent (1 IE μL -1 ) under an argon stream in a clean conical bottom vial.…”

Section: Collection Of Volatiles From Male and Female Insectsmentioning

confidence: 99%

Male-specific volatiles released by the brazilian papaya weevil, pseudopiazurus obesus: partial identification and evidence of an aggregation pheromone

Zarbin¹,

Moreira²,

Haftmann³

et al. 2007

J. Braz. Chem. Soc.

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A resposta comportamental de machos e fêmeas adultos de Pseudopiazurus obesus (Boheman, 1838) (Coleoptera: Curculionidae) a várias fontes de odores foi avaliada em um olfatômetro de tubo em Y. insetos machos e fêmeas foram significativamente mais atraídos para uma combinação dos voláteis emitidos pelos machos da espécie e pela planta hospedeira, sugerindo a existência de um feromônio de agregação produzido pelos machos. Análises comparativas dos voláteis emitidos pelos machos e fêmeas revelaram três compostos machoespecíficos, em uma relação de 77:14:9, dando suporte químico às observações comportamentais. (1R,2S)-(+)-Grandisal e (1R,2S)-(+)-grandisol foram identificados como sendo os compostos majoritário e intermediário, respectivamente, enquanto que a estrutura química do componente minoritário, que parece ser um novo derivado do grandisol, ainda necessita ser determinada. Estes três compostos são os principais candidatos a feromônio na espécie.The behavioral responses of adult male and female Pseudopiazurus obesus (Boheman, 1838) (Coleoptera: Curculionidae) to several odour sources were evaluated in a Y-tube olfactometer. Males and females insects were significantly more attracted to a combination of volatiles released by males of the species and host plant, suggesting the existence of a male-produced aggregation pheromone. Comparative analysis of the volatiles released by males and females revealed three male-specific compounds, in a ratio of 77:14:9, providing a chemical support to the behavioral observations. (1R,2S)-(+)-Grandisal and (1R,2S)-(+)-grandisol were identified as the major and intermediate compounds, respectively, while the chemical structure of the minor compound, that seems to be a new grandisol derivative, still remains to be determined. These three compounds are the most important pheromone candidates in the species.

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Metodologias gerais empregadas no isolamento e identificação estrutural de feromônios de insetos

Cited by 32 publications

References 0 publications

Evaluation of porous silica glasses as insect pheromone dispensers

Evaluation of porous silica glasses as insect pheromone dispensers

Identification and Synthesis of the Male-Produced Sex Pheromone of the Soldier BeetleChauliognathus fallax(Coleoptera: Cantharidae)

Male-specific volatiles released by the brazilian papaya weevil, pseudopiazurus obesus: partial identification and evidence of an aggregation pheromone

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