Os DNA sintéticos anti-sentido

Abstract

Recebido em 4/6/97; aceito em 7/11/97 ANTISENSE SYNTHTETIC DNA. One old dream of the chemist in the field of the drug research is to create molecules capable of reaching their target with the precision of a missile. To accomplish it these molecules must have the propriety of distinguishing qualitative differences between healthy and diseased cells. A therapy based on this principle, able of eradicating specifically defective cells, or cells affected by a pathogen has an enormous advantage with the regard to the classical approach in which the cytotoxic drugs merely exploit quantitative biochemical and kinetic differences between abnormal and normal cells. We present in this article a review on the chemical synthesis of analogues of desoxyribonucleotides and on results obtained on the specific and irreversible inhibition of undesired genetic expression using the antisense principle.

Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide

“…1 The use of oligonucleotides has been considered a new strategy for the treatment of malaria given the fact that these single strand nucleic acids can interfere in protein synthesis, thus inhibiting parasite growth. 2,3 However, the use of oligonucleotides is hampered in vivo due to their very low penetration ability 4,5 and susceptibility against nuclease degradation. 6,7 In addition to chemical modifications, oligonucleotides association with positively-charged colloidal carriers has been extensively investigated to circumvent such drawbacks.…”

Seleção de microRNA e proteína alvo envolvidos com a função cardíaca de ratos infartados em resposta ao treinamento aeróbico

“…Nesta abordagem, estas moléculas são exógenas e o seu efeito é transitório (CRAVADOR, 1998;DAVIS et al, 2008). (RAPER et al, 2003).…”