Avaliação quantitativa de cardenolídeos no cultivar experimental de Digitalis lanata do maciço do itatiaia e perspectivas de seu emprego industrial

Braga, Fernão Castro; Kreis, Wolfgang; Récio, Rubens Almeida; Oliveira, Alaı́de Braga de

doi:10.1590/s0100-40421997000500008

Cited by 2 publications

(1 citation statement)

References 10 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[2][3][4][5][6][7][8][9][10][11][12][13] There are some methods reported for digoxin determination after a dissolution test. [14][15][16] In the United States Pharmacopoeia (USP), 0.1 M hydochloric acid (HCl) and in the Japanese Pharmacopoeia (JP), diluted HCl is used as the dissolution medium, 17,18 while distilled water is employed as the dissolution medium in the British Pharmacopeia (BP).…”

Section: Introductionmentioning

confidence: 99%

An HPLC method for the determination of digoxin in dissolution samples

Milenković¹,

Marinković²,

Sibinovic³

et al. 2010

JSCS

View full text Add to dashboard Cite

An HPLC method for digoxin quantification in dissolution samples obtained as per the official British Pharmacopeia (BP) method is presented in this paper. The chromatography was performed at 20 °C on a Symmetry C18; 3.5 ìm, 75 x 4.6 mm column with water - acetonitrile (72 : 28, v/v), as the mobile phase and UV detection at 220 nm. The method was found to be selective, linear, accurate and precise in the specified ranges. The LOD and LOQ were 0.015 μg mL-1 and 0.050 μg mL-1, respectively. Robustness testing was conducted to evaluate the impact of minor changes in the chromatographic parameters (i.e., acetonitrile fraction, flow rate of the mobile phase, column temperature and column length) on the characteristics of the digoxin peak. A. full factorial design (24) was used to investigate the influence of the four variables The presented HPLC method was applied in quality and stability testing of Digoxin tablets 0.25 mg

show abstract