Limpeza clonal de mudas de videira infectadas por Xanthomonas campestris pv. viticola

Silva, Adriano Márcio Freire; Melo, Natoniel Franklin de; Souza, Elineide Barbosa de; Coelho, Ângela Katiusia; Mariano, Rosa de Lima Ramos

doi:10.1590/s0100-29452013000100036

Cited by 6 publications

(8 citation statements)

References 8 publications

(3 reference statements)

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“…[38]. In Vitis vinifera, the addition of streptomycin at 100 mg L -1 did not control the manifestation of bacterial canker, and after 40 days of in vitro cultivation, 100% of the explants remained with the bacterium [37]. Unlike these reports, our results for Eucalyptus microcorys showed that the addition of streptomycin at 250 mg L -1 did not reduce microbial manifestation in the culture medium; whereas at 100 mg L -1 , microbial manifestation decreased in 65% of the explants of individual 2.…”

Section: Reduction Of Microbial Manifestationmentioning

confidence: 99%

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WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

Atala

Faria

Molinari

et al. 2021

Preprint

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Micropropagation is one of the main applications of tissue culture of plants. It allows rejuvenation and, consequently, improves explant rooting of woody species. However, one of its main problems is the risk of microbial manifestation. Here, we aimed to evaluate the survival and the reduction in microbial manifestations on in vitro cultures of Eucalyptus microcorys with the addition of chemical agents (sodium hypochlorite or streptomycin) to WPM culture medium. We used in vitro cultivated explants from adventitious shoots from two E. microcorys mother plants, over at 44 years-old. We drew from the multiplication phase explants with clumps of 8 to 12 shoots that displayed bacterial manifestation and submitted them to microbe control, adding different chemical agents at different concentrations to the culture medium: sodium hypochlorite (NaClO) and the antibiotic streptomycin. The results indicated that the best concentrations to reduce the manifestation of microorganisms on in vitro cultivation were 100 mg L− 1 of streptomycin and sodium hypochlorite with 0.003% of active chlorine, since these concentrations maintained 100% of the explants alive and reduced microbial manifestation more efficiently.

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Section: Reduction Of Microbial Manifestationmentioning

confidence: 99%

“…Streptomycin has been tested for controlling the manifestation of microorganisms on in vitro cultures of several species, such as Helianthus tuberosus [32], Persea americana [33], Solanun tuberosum [34], Rauwol a serpentina [35], Guadua angustifolia Kunth [36], Vitis vinifera [37], Butea monosperma (Lam.) Kuntze var.…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

Atala

Faria

Molinari

et al. 2021

Preprint

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“…Thermal treatments could also be used in plants of Vitis. Silva et al (2013) failed to eliminate Xcv from in vitro plants using thermotherapy at 38°C for 30 days. Thermo-therapeutic treatments with dormant plant materials of Vitis at 48-52°C for 30-45 min (according to grapevine cultivars) are recommended for other bacterial plant pathogens (EFSA PLH Panel, 2015).…”

Section: Potential Risk Reducing Optionsmentioning

confidence: 99%

Risk assessment of Xanthomonas citri pv. viticola for the EU

Bragard¹,

Baptista²,

Chatzivassiliou³

et al. 2022

EFS2

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Following a request from the European Commission, the EFSA Panel on Plant Health performed a risk assessment of Xanthomonas citri pv. viticola (Xcv). This pest causes bacterial canker of grapevine and is reported from Brazil and India. Two scenarios were considered: scenario A0 (current practice) and A2 (additional control measures). For the fresh grape import pathway, scenario A0 results in an order of magnitude of about one entry per 10 years (median; 90% uncertainty interval between ca. one entry per 18,000 years and ca. five entries per year). For the Vitis spp. plants for planting for research/breeding purposes import pathway, the risk of entry is several orders of magnitude smaller than the risk due to fresh grape import. This outcome is also obtained under scenario A2. The key entry uncertainties include import volume and transfer (for plants for planting), transfer and the disaggregation factor (for fresh grapes) and the limited availability of epidemiological data. The extent of the area favourable for Xcv establishment in the EU is uncertain, illustrating the limitations of climate suitability assessments when based on few data points and little epidemiological information. Nevertheless, the risk of Xcv establishment is only slightly lower than the risk of Xcv entry, i.e. no major establishment constraints are expected for most entries. Similarly, the risk of Xcv establishment is assessed as only slightly lower under current climate compared to the climate of 2041–2060. For grapevine growing areas in the EU with average yearly temperature above 17°C, the lag phase between establishment and spread is expected to be about 3 years (median; 90% range between ca. 6 months and ca. 6 years). Under the same scenario, the rate of spread by natural means is assessed to be ca. 300 m/year (median; 90% range between ca. 35 and ca. 800 m/year). The spread rate would be considerably higher considering movements of plants and cutting tools or machinery. The percentage of grapevine plants infected by Xcv in production sites as yearly average over a 30‐year production cycle is estimated to be ca. 17% (median; 90% range between ca. 1.5% and ca. 46%) in table grapes and ca. 12% (median; 90% range between ca. 0.7% and ca. 37%) in wine grapes. Impacts have been reported to be severe in Brazil and India, but the estimates provided here show that there is considerable uncertainty about expected impacts in the EU.

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“…Atualmente, a técnica de cultura de tecidos é muito utilizada para propagação de plantas livres de patógenos e, no caso da videira, possibilita a obtenção de plantas com boa regeneração e livres de Xcv (SILVA et al, 2013). No entanto, requer longo tempo e ainda não está sendo utilizada em larga escala no Vale do Submédio São Francisco.…”

Section: Introductionunclassified

Efeito do tratamento de bacelos de videira 'Red Globe' no controle do cancro bacteriano causado por Xanthomonas campestris pv. viticola

et al. 2014

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A disseminação de Xanthomonas campestris pv. viticola (Xcv), agente do cancro bacteriano da videira, ocorre, dentre outras formas, por meio de mudas e bacelos infectados. Foi estudada a obtenção de material propagativo livre do patógeno, testando a eficiência do tratamento de bacelos com termoterapia, bactericidas e sanitizantes. Os isolados de Xcv foram testados quanto à patogenicidade e realizado o teste de sensibilidade in vitro aos produtos, em diferentes concentrações. A erradicação de Xcv em bacelos de videira foi testada em experimentos com termoterapia (50ºC por 30 e 40 min; 53ºC por 5 e 10 min); bactericidas [oxitetraciclina+sulfato de cobre (150+2.000; 165+2.200; 180+2.400 e 195+2.600 mg L-1 de H2O) e oxitetraciclina (600; 700; 800 e 900 mg L-1)]; e sanitizantes [cloreto de dodecildimetil amônio (600; 1.200; 1.800; 2.400 e 3.000 µL L-1); hipoclorito de sódio (5.000; 10.000; 20.000; 30.000 e 40.000 µL L-1) e cloreto de benzalcônio (125; 167;250; 334 e 500 µL L-1)]. Foram avaliados período de incubação, incidência e severidade da doença. O bactericida oxitetraciclina e os sanitizantes cloreto de dodecildimetil amônio e hipoclorito de sódio proporcionaram os maiores halos de inibição de Xcv in vitro. No entanto, apesar dos diversos tratamentos testados, não foi possível recomendar tratamento termoterápico ou produto que erradicasse Xcv de bacelos infectados. Porém, ficou confirmada a grande importância destes na disseminação do agente do cancro bacteriano da videira.

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Limpeza clonal de mudas de videira infectadas por Xanthomonas campestris pv. viticola

Cited by 6 publications

References 8 publications

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

Risk assessment of Xanthomonas citri pv. viticola for the EU

Efeito do tratamento de bacelos de videira 'Red Globe' no controle do cancro bacteriano causado por Xanthomonas campestris pv. viticola

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