Análise da variabilidade genética de acessos de Psidium spp. (Myrtaceae) avaliados quanto à reação a Meloidogyne enterolobii¹

Abstract

RESUMO -Este trabalho teve por objetivo a caracterização molecular de 13 acessos de Psidium spp. (Myrtaceae) identificados previamente quanto à reação ao nematoide da goiabeira. A extração do DNA das amostras foi executada conforme o protocolo de Shillito e Saul (1988). Os marcadores moleculares do tipo fAFLP, foram obtidos utilizando-se do 'fAFLP Regular Plant Genomes Fingerprinting Kit' (Applied Biosystems do Brasil Ltda.) onde foram testadas 24 combinações seletivas de primers, das quais 18 apresentaram amplificação que gerou 272 marcadores polimórficos. Para a análise dos marcadores, foram utilizados os softwares GeneScan (ABI Prism versão 1.0) e Genotyper (ABI Prism versão 1.03), e os dados coletados foram transformados em matriz binária que foi analisada no software PAUP (Phylogenetic Analysis Using Parcimony -versão 3.01). Foram também calculados índices de distância genética intra e interespecífica entre os materiais. Verificou-se que os marcadores AFLP foram eficientes na discriminação dos acessos entre si, bem como apontou similaridade genética entre os acessos identificados como resistentes ao nematoide Meloidogyne enterolobii, característica esta passível de exploração no futuro. Termos para indexação: resistência de plantas a doenças, fruta tropical, Psidium guajava, nematoide de galha, marcadores genéticos. ANALYSIS OF GENETIC VARIABILITY OF Psidium spp. (MYRTACEAE) ACCESS EVALUATED FOR RESISTANT TO Meloidogyne enterolobiiABSTRACT -This study aimed to characterize molecular of 13 accessions of Psidium spp. (Myrtaceae) that was been identified for the reaction to rootknot guava nematode. The DNA extraction of the samples was carried according to the protocol of Shillito & Saul (1988). The molecular markers type fAFLP, were obtained from 'fAFLP Regular Plant Genomes Fingerprinting Kit' (Applied Biosystems from Brasil Ltda.) and were tested 24 selectives combinations of primers, of which 18 showed amplification that produced 272 polymorphic markers. To the analysis of the markers were employed the softwares GeneScan (ABI Prism versão 1.0) and Genotyper (ABI Prism version 1.03), and the data collected were transformed into a binary matrix that was analyzed in the software PAUP (Phylogenetic Analysis Using Parcimony -version 3.01).Were calculated genetic distance índex intra and interespecific between the genotipes. It was found that the AFLP markers were efficient in the discrimination between accessions, as well as in showing genetic similarity among accessions identified as resistant to the nematode Meloidogyne enterolobii, which could be discussed in the future.

Characterization of Eugenia uniflora accessions: a native species with great commercial potential in America

“…Several types of molecular markers have been used for the analysis of genetic diversity in different species, but there are few records for native species of the South of Brazil (Franzon & Raseira 2004), mainly species of the Myrtaceae family; which may be associated with the presence of polyphenols that hinder DNA extraction (Almeida et al 2012). In this study, RAPD markers were used because it is a simple, fast, and relatively inexpensive technique with informative potential, although it presents repeatability problems (Areias et al 2006).…”