In vitro organogenesis in some citrus species

Schinor, Evandro Henrique; Azevedo, Fernando Alves de; Filho, Francisco de Assis Alves Mourão; Mendes, Beatriz Madalena Januzzi

doi:10.1590/s0100-29452011005000050

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…The highest number of shoots were obtained in 1.5 mg/l BAP, 0.5 mg/l BAP + 1 mg/l Kn and 0.5 mg/l Kn + 1 mg/l BAP + 1 mg/l 2,4-D. Similar result was reported in Citrus volkameriana (Schinor et al 2011) where the number of shoots increased when 1.5 mg/l BAP was used. The multiplication of shoots appeared to be affected by the use of BAP.…”

Section: Resultssupporting

confidence: 84%

Micropropagation of Citrus indica Tanaka using Shoot tips from Mature Trees

Chhetri

Mathew

Pereira

2021

Plant Tissue Cult. & Biotech.

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A study was conducted to standardize a protocol for in-vitro direct regeneration and mass multiplication of Citrus indica Tanaka using shoot tip explants excised from mature trees. Shoot tips were inoculated in MS medium supplemented with varying concentrations and combinations of cytokinins and auxins. MS media when fortified with BAP 0.5 mg/l and 0.5 mg/l BAP + 1.0 mg/l Kn were found to be the best treatments for shoot initiation while MS supplemented with 1 mg/l IBA; 0.5 mg/l NAA + 0.5 mg/l IBA and 0.5 mg/l NAA + 0.5 mg/l IAA were the best treatments for root induction. Among the different media used for hardening, 100 % survivability was obtained when plantlets were hardened using vermicompost as the potting medium. Subsequently, these plantlets were transferred to larger pots and acclimatization was achieved gradually in outdoor conditions. Plant Tissue Cult. & Biotech. 31(1): 13-23, 2021 (June)

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Section: Resultssupporting

confidence: 84%

Micropropagation of Citrus indica Tanaka using Shoot tips from Mature Trees

Chhetri

Mathew

Pereira

2021

Plant Tissue Cult. & Biotech.

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“…The results of the table showed the significant effect of the triple interference of the experimental factors in the characteristic of plant height, as the interference treatment the rootstock of Volkameriana and coconut liquid 200 g L -1 and yeast extract at a concentration of 8 g L -1 achieved the highest rate of 37.863 g compared to the interference treatment of the rootstock of Citrus aurantium and coconut liquid 0 g L -1 and yeast extract 0 g L -1 as it recorded the lowest rate of 17.903g an increase of 111.489% . Tables (1,2,3,4,5,6) show the superiority of the Volkameriana rootstock over the Citrus aurantium rootstock in the studied vegetative traits, which may be due to the nature of the genetic structure of the root and may be due to the high specifications that characterize this rootstock and its positive effect on the grafts growing on it and its compatibility with most types of citrus fruits as well as its resistance to rapid deterioration disease, wood pecking, and viroid bark scaling disease [9] and [20]. Also, this root is considered the best in terms of characteristics compared to Citrus aurantium and other rootstocks, and this is consistent with what was confirmed by [21] and [22], where they confirmed that it is the best in terms of vegetative growth characteristics compared to the rootstock of Citrus aurantium.…”

Section: Dry Weight Of Vegetative Total (G)mentioning

confidence: 99%

Effect of Spraying with Coconut Liquid and Yeast Extract on Vegetative Growth Traits of Citrus rootstock (Citrus aurantium and Volkameriana)

Al-Muhanna,

Al-Rubaei,

Hassan

2023

IOP Conf. Ser.: Earth Environ. Sci.

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The experiment was conducted in the plant canopy at Horticulture and Landscape Department, College of Agriculture / University of Kerbala, Iraq for the period from mid-February to the end of June 2023 using Randomized Complete Block Design (R.C.B.D) as a factorial experiment with three factors: citrus rootstock (Citrus aurantium and Volkameriana) and coconut liquid extract with three concentrations of (0, 100, 200) ml L-1 and dry yeast extract in three concentrations (0, 4, 8) g L-1 and with three replications. The most important results indicated the superiority of the Volkameriana rootstock in most of the studied growth characteristics (plant height, stem diameter, number of leaves, leaf area, shoot dry weight), while the treatment of coconut liquid 200 ml L-1, as well as the treatment of dry yeast extract with a concentration of 8 g L-1 achieved a significant superiority for the above studied traits and gave the highest rates. The two-way interaction treatment between (rootstock and coconut liquid) and (rootstock + dry yeast extract) not a significant, as for and (coconut liquid 200 ml L-1 + dry yeast extract at a concentration of 8 g L-1) achieved the highest rates in most of the studied traits. The triple interaction treatment (Volkameriana rootstock + coconut liquid 200 ml L-1 + dry yeast extract at a concentration of 8 g L-1) achieved significant superiority in most of the studied growth characteristics (seedling height, stem diameter, number of leaves, leaf area, shoot dry weight). While the triple overlap treatment (Citrus aurantium + coconut liquid 200 ml L-1 + dry yeast extract at concentration of 8 g L-1) achieved the highest rate of relative moisture content of the leaves, as it gave the highest rates (38.833 cm, 4.2033 mm, 78.667 leaves seedlings-1, 2398.67 cm2, 37.863 g seedlings-1) (70.3 15%, respectively.

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“…However, the efficiency of existing protocols may vary depending on the genotype used. Some species and varieties have shown to be recalcitrant to in vitro process in citrus, especially genotypes used as rootstocks (Tavano et al, 2009;Schinor et al, 2011). Although the induction of embryogenic callus lines, especially from ovule tissue, has been extensively investigated in citrus, there are only a few reports on embryogenic callus induction and protoplast isolation in recalcitrant rootstocks such as Volkameriana (Citrus volkameriana L.).…”

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confidence: 99%

Efficient protoplast isolation from ovule-derived embryogenic callus inCitrusvolkameriana

Çimen¹

2020

Turk J Agric For

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The present study reports on the isolation of viable protoplast from ovule-derived embryogenic calli of Volkameriana (Citrus volkameriana L.), which is a rootstock in high demand for lemon production.Ovules of C. volkameriana isolated at 3 different immature fruit stages, comprising4, 8, and 12 weeks after anthesis (WAA),were cultured on5 different media in order to produce embryogenic callus lines as a source material for protoplast isolation. EME medium (MT basal medium + 0.5 gL–1 malt extract), with the addition of phytohormones [kinetin (KIN), 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP)] at different concentrations, were tested for callogenesis. According to 2-way ANOVA, significant effects were determined as a result of the immature fruit stage and type of culture media (P ≤ 0.01) on the callogenesis and embryogenic callus induction frequency. First, callus formation was recorded after 4 WAA on medium comprising EME + 2,4-D (1.0 mg L–1) + BAP (0.5 mg L–1). Callus induction frequency was the highest (90.00%) in the same culture medium when the ovules wereculturedat8 WAA. In addition, culturing the ovules isolated from 12 WAA immature fruits of C. volkameriana resulted in the highest indirect somatic embryogenesis (24%). Embryogenic callus initiation was the highest (25.56%) using EME + KIN (1.0 mg L–1) and ovules cultured at 8 WAA (14%) resulted in the highest embryogenic callus formation. Effects of different enzyme concentrations on the efficiency of protoplast isolation were calculated using the hemocytometer cell counting method. The combination of 2% cellulase and 0.2% pectinase gave the highest numbers of protoplasts, at 12.33 × 105protoplast/mL. Embryogenic callus lines obtained by culturing ovules of C. volkameriana yielded high-quality protoplasts after isolation and could be useful as a protoplast source for further somatic hybridization studies.

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In vitro organogenesis in some citrus species

Cited by 7 publications

References 12 publications

Micropropagation of Citrus indica Tanaka using Shoot tips from Mature Trees

Micropropagation of Citrus indica Tanaka using Shoot tips from Mature Trees

Effect of Spraying with Coconut Liquid and Yeast Extract on Vegetative Growth Traits of Citrus rootstock (Citrus aurantium and Volkameriana)

Efficient protoplast isolation from ovule-derived embryogenic callus inCitrusvolkameriana

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