Caracterização molecular de populações de Butia capitata (Arecaceae) do Sul do Brasil através de marcadores AFLP

Abstract

O gênero Butia (Arecaceae) é um pequeno gênero subtropical com espécies no sul da América do Sul, considerado ornamental. Além disso, seus frutos são apreciados pelo sabor e aroma peculiares. Porém, no Rio Grande do Sul, as populações naturais sofrem com o avanço das atividades rurais e da construção imobiliária. O objetivo deste trabalho foi caracterizar oito populações de Butia capitata ocorrentes no Rio Grande do Sul através de marcadores moleculares do tipo AFLP. Pela análise molecular da variância, foi possível verificar que 83,68% da variabilidade genética são atribuídos à variação entre populações e 13,67% são atribuídos a diferenças entre populações dentro de regiões. A análise comparativa entre as oito populações feita de duas a duas demonstrou que são significativas as diferenças entre 15 populações, com média de 14,72% da variação molecular atribuída às diferenças entre populações. Este resultado indica a presença de variabilidade genética distribuída entre todas as populações, sem subdivisão decorrente de isolamento geográfico.

Agricultural Biodiversity in Southern Brazil: Integrating Efforts for Conservation and Use of Neglected and Underutilized Species

“…The plant is also used in landscaping gardens and yards. In the last five to 10 years, a number of research investigations have shed light on this very useful multi-purpose plant and advanced our understanding on its biology, field management and use in southern Brazil [35][36][37][38][39][40][41][42][43][44][45]. Considering the current loss of natural populations of this palm in the Pampa biome, actions for the sustainable conservation and management of the remaining populations are being carried out, with a view at studying the population dynamics, conserving genetic variability, recording innovative use of germplasm by local populations and providing better characterization and in situ conservation, while adopting an integrated plant production and livestock raising approach.…”

OCURRENCE OF Revena plaumanni BONDAR, 1943 (Coleoptera: CURCULIONIDAE) IN PINDO PALM FRUIT

“…In the past half century this plant had great economic importance, and its leaf fiber used in the upholstery industry (Tonietto et al, 2009). Currently, Büttow et al, (2010) reported that the most used part of the Pindo palm fruit is consumed in natura or used in the preparation of liquor, jelly, ice cream, among other products.…”

Molecular techniques for the study and diagnosis of parasite infection

“…This technique is highly efficient because of the possibility of analyzing a large number of bands simultaneously, with extensive coverage of the genome (77). This method of molecular diagnosis employs PCR to selectively amplify the groups of restriction fragments of totally digested genomic.…”

“…Polymorphisms are identified based on the presence or absence of DNA fragments by polyacrylamide gel analysis (7). The advantages of this technique are the ability to search an entire genome for polymorphisms, the reproducibility of the method, and the possibility of being used against parasites about which there is no prior genetic information (7,77,78).…”

“…Polymorphisms are identified based on the presence or absence of DNA fragments by polyacrylamide gel analysis (7). The advantages of this technique are the ability to search an entire genome for polymorphisms, the reproducibility of the method, and the possibility of being used against parasites about which there is no prior genetic information (7,77,78).Studies have demonstrated its application to differentiate isolates of C. parvum into two distinct genotypes, as well as strains of Leishmania belonging to cutaneous leishmaniosis (CL) and visceral leishmaniosis (VL) (7). Analysis based on AFLP polymorphic markers have revealed high genetic variability among the genome species of Leishmania major, L. tropica and L. donovani, which was sufficient to distinguish between CL and VL (79).…”