Micropropagação de porta-enxertos híbridos de Vitis labrusca x Vitis rotundifolia com resistência à pérola-da-terra (Eurhizococcus brasiliensis Hempel, Hemiptera: Margarodidae)

Bernd, Regina B; Trivilin, Ana Paula; Camargo, U. A.; Czermainski, A. B. C.

doi:10.1590/s0100-29452007000200031

Cited by 7 publications

(7 citation statements)

References 9 publications

(10 reference statements)

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“…The 'Paulistinha' genotype had a higher shoot production than the 'Baco Blanc' one, with averages of 5.3 and 2.5 shoots per explant, respectively. Bernd et al (2007), while evaluating grapevine hybrids, obtained an average of 4.9 shoots per explant in a culture medium supplemented with 3.0 µmol L -1 of BAP. Torregrosa & Bouquet (1995), in a study with different grapevine hybrids, observed that a concentration of 4.4 µmol L -1 of BAP resulted in better rates of multi-budding.…”

Section: Resultsmentioning

confidence: 99%

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Short-term storage in vitro and large-scale propagation of grapevine genotypes

Silva

Luis

Scherwinski‐Pereira

2012

Pesq. agropec. bras.

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-The objective of this work was to evaluate the large-scale propagation of grapevine genotypes after short-term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short-term storage, the shoots were propagated in up to five successive subcultures, to assess the large-scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short-term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with averages of 4.9 and 4.8 shoots per explant, respectively. In the rooting phase, the best results for number of roots were obtained using a culture medium supplemented with 0.4 µmol L -1 of IBA, with an average of three roots per shoot. During the acclimation phase, a survival rate higher than 95% was achieved after 30 days in the greenhouse. Grapevine genotypes maintained for six months in vitro, at 20 o C, can be micropropagated in large scale.

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Section: Resultsmentioning

confidence: 99%

“…For some cultivars, the presence of auxin in the culture medium is essential for good rooting. Bernd et al (2007), using a culture medium supplemented with NAA at a concentration of 8.05×10…”

Section: Resultsmentioning

confidence: 99%

Short-term storage in vitro and large-scale propagation of grapevine genotypes

Silva

Luis

Scherwinski‐Pereira

2012

Pesq. agropec. bras.

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“…This response was evaluated by the number of buds regenerated and the number of shoots per bud obtained. Bernd et al ( 2007 ) evaluated grapevine hybrids and obtained an average of 4.9 shoots per explant in a culture medium supplemented with 3.0 μmol L −1 of BAP. In another study with a different grapevine hybrid, multi-budding was observed at a concentration of 4.4 μmol L −1 of BAP (Torregrosa and Bouquet 1995 ).…”

Section: Discussionmentioning

confidence: 99%

“…In agreement with our results, Lee and Wetzstein ( 1990 ) showed that in ‘Muscadine’ grape, the percentage of rooting was greater in the treatments including IBA compared with that without hormones, although this behavior was genotype dependent. Bernd et al ( 2007 ) utilized a medium supplemented with NAA at a concentration of 8.05 × 10 −3 μmol L −1 to induce rooting of grapevine shoots induced by the utilization of BAP at concentrations of 3.5 and 10 μmol L −1 . Silva et al ( 2012 ) obtained the highest number of roots on the grapevine at a concentration of 0.4 μmol L −1 of IBA, with an average of 3.3 roots per explant.…”

Section: Discussionmentioning

confidence: 99%

Surface disinfection procedure and in vitro regeneration of grapevine (Vitis vinifera L.) axillary buds

et al. 2016

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Establishment of an efficient explants surface disinfection protocol is essential for in vitro cell and tissue culture as well as germplasm conservation, such as the case of Grapevine (Vitis spp.) culture. In this research, different procedures for disinfection and regeneration of field-grown grapevine cv. ‘Flame seedless’ axillary buds were evaluated. The buds were disinfected using either NaOCl or allyl, benzyl, phenyl and 2-phenylethyl isothiocyanates. Two different media for shooting and four media for rooting were tested. Shoot and root development per buds were registered. The best disinfection procedure with 90 % of tissue survival involved shaking for 60 min in a solution containing 20 % Clorox with 50 drops/L Triton® X-100. These tissues showed the potential to regenerate a complete plant. Plant regeneration was conducted using full strength Murashigue and Skoog (MS) medium supplemented with 8 µM benzyl aminopurine for shoot induction and multiplication, whereas rooting was obtained on half strength MS supplemented with 2 mg L−1 of indole-3-butyric acid and 200 mg L−1 of activated charcoal. In this work, it was designed the protocols for obtaining sterile field-grown grapevine buds and in vitro plant development. This methodology showed potential to produce vigorous and healthy plants in 5 weeks for clonal grapevine propagation. Regenerated plants were successfully established in soil.

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“…A propagação vegetativa de plantas pode ser uma técnica eficiente para obtenção de novos propágulos, além de conservar toda a característica genética da planta matriz (SOUZA et al, 2013). A estaquia consiste em produzir uma nova planta a partir do segmento de uma planta matriz, porém algumas espécies podem apresentar baixa capacidade de enraizamento, dificultando o estabelecimento de novas plantas (BERND et al, 2007), tais como deterioração de propágulos, perda de turgidez das estacas, ausência de primórdios radiculares, entre outros (PETRY et al, 2012).…”

Section: Introductionunclassified

Viabilidade do extrato aquoso de Cyperus rotundus como indutor de enraizamento em estacas de videira em comparação com hormônios sintéticos

Thiesen

Schmidt

Holz

et al. 2019

ABC

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O objetivo do trabalho foi avaliar o efeito do extrato aquoso de tubérculos de tiririca (Cyperus rotundus), comparando-o com a ação dos hormônios vegetais ácido indolbutírico e ácido naftalenoacético, em estacas de videira (Vitis vinifera L. [var. Bordô e Niágara]). O experimento foi conduzido em delineamento inteiramente casualizado, em esquema fatorial 2 x 4, sendo duas variedades (Bordô e Niágara) e quatro indutores de enraizamento (ácido indolbutírico, ácido naftalenoacético, extrato aquoso de tiririca e água destilada – controle). Após 125 dias de condução do experimento, foram avaliados: número de folhas, número de raízes, número de brotos, comprimento da raiz, comprimento da parte aérea e massa seca da raiz e das folhas. O extrato aquoso de tiririca promoveu resultados satisfatórios sobre o número de brotos e o comprimento de raízes, não diferindo estatisticamente dos hormônios vegetais sintéticos. Entretanto a resposta foi diferente entre as variedades, não ocasionando incremento significativo de massa seca de raiz e de folhas. O número de folhas das variedades de videira é dependente do genótipo, assim como a matéria seca de raiz e de folhas. O extrato aquoso de tiririca pode ser uma alternativa sustentável para promover o enraizamento de estacas, em função da presença de substâncias que estimulam o enraizamento.

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Micropropagação de porta-enxertos híbridos de Vitis labrusca x Vitis rotundifolia com resistência à pérola-da-terra (Eurhizococcus brasiliensis Hempel, Hemiptera: Margarodidae)

Cited by 7 publications

References 9 publications

Short-term storage in vitro and large-scale propagation of grapevine genotypes

Short-term storage in vitro and large-scale propagation of grapevine genotypes

Surface disinfection procedure and in vitro regeneration of grapevine (Vitis vinifera L.) axillary buds

Viabilidade do extrato aquoso de Cyperus rotundus como indutor de enraizamento em estacas de videira em comparação com hormônios sintéticos

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