Expressão transiente do gene gus, sob regulação de quatro promotores, em diferentes tecidos de mamoeiro (Carica papaya L.) e videira (Vitis sp.)

Pinto, Adelar Almeida; Coelho, M. C. F.; Júnior, Manoel Teixeira Souza; Guerra, Miguel Pedro

doi:10.1590/s0100-29452002000300058

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…Because practical methods for transforming papaya have been developed and the biotechnology is becoming well refined (Fitch et al, 1990;Pinto et al, 2002;Zhu et al, 2004;Wall et al, 2004), and transgenic commercial lines have been approved and available since 1998 (Cai et al, 1999;Ying et al, 1999;Gonsalves, 2000), papaya is a focus for improvement using genetic engineering approaches. Programs are using such tools to transform various varieties, for example co-ordinated under the Papaya Biotechnology Network of Southeast Asia (ISAAA, 2001a), with a current focus on delayed ripening characteristics and resistance to PRSV.…”

Section: Molecular Approaches For Agronomic Improvementmentioning

confidence: 99%

Section 7 - Bread Wheat (TRITICUM AESTIVUM)

2006

Safety Assessment of Transgenic Organisms, Volume 1

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The OECD is a unique forum where the governments of 30 democracies work together to address the economic, social and environmental challenges of globalisation. The OECD is also at the forefront of efforts to understand and to help governments respond to new developments and concerns, such as corporate governance, the information economy and the challenges of an ageing population. The Organisation provides a setting where governments can compare policy experiences, seek answers to common problems, identify good practice and work to co-ordinate domestic and international policies.The OECD member countries are: Australia, Austria, Belgium, Canada, the Czech Republic, Denmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Japan, Korea, Luxembourg, Mexico, the Netherlands, New Zealand, Norway, Poland, Portugal, the Slovak Republic, Spain, Sweden, Switzerland, Turkey, the United Kingdom and the United States. The Commission of the European Communities takes part in the work of the OECD.OECD Publishing disseminates widely the results of the Organisation's statistics gathering and research on economic, social and environmental issues, as well as the conventions, guidelines and standards agreed by its members. © OECD 2006 No reproduction, copy, transmission or translation of this publication may be made without written permission. Applications should be sent to OECD Publishing: rights@oecd.org or by fax (33 1) 45 24 13 91. Permission to photocopy a portion of this work should be addressed to the Centre français d'exploitation du droit de copie, 20, rue des Grands-Augustins, 75006 Paris, France (contact@cfcopies.com). This book is published on the responsibility of the Working Group on Harmonisation of Regulatory Oversight in Biotechnology, which is a subsidiary group of the Chemicals Committee and Working Party on Chemicals, Pesticide and Biotechnology of the OECD. Foreword 3 FOREWORDGenetically engineered crops (also known as transgenic crops) such as maize, soybean, rapeseed and cotton have been approved for commercial use in an increasing number of countries. During the period from 1996 to 2005, for example, there was more than fifty-fold increase in the area grown with transgenic crops worldwide, reaching 90 million hectares in 2005.1 Such approvals usually follow a science-based risk/safety assessment.The environmental safety/risks of transgenic organisms are normally based on the information on the characteristics of the host organism, the introduced traits, the environment into which the organism is introduced, the interaction between these, and the intended application. The OECD's Working Group on Harmonisation of Regulatory Oversight in Biotechnology decided at its first session, in June 1995, to focus its work on identifying parts of this information, which could be commonly used in countries for environmental safety/risk assessment to encourage information sharing and prevent duplication of effort among countries. Biosafety Consensus Documents are one of the major outputs of its work.B...

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Section: Molecular Approaches For Agronomic Improvementmentioning

confidence: 99%

Section 7 - Bread Wheat (TRITICUM AESTIVUM)

2006

Safety Assessment of Transgenic Organisms, Volume 1

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Isolation and characterization of the promoter sequence of a cassava gene coding for Pt2L4, a glutamic acid-rich protein differentially expressed in storage roots

Souza

Fj²,

Ec³

et al. 2009

Genet. Mol. Res.

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ABSTRACT. Cassava is one of the most important tropical food crops for more than 600 million people worldwide. Transgenic technologies can be useful for increasing its nutritional value and its resistance to viral diseases and insect pests. However, tissuespecific promoters that guarantee correct expression of transgenes would be necessary. We used inverse polymerase chain reaction to isolate a promoter sequence of the Mec1 gene coding for Pt2L4, a glutamic acid-rich protein differentially expressed in cassava storage roots. In silico analysis revealed putative cis-acting regulatory elements within this promoter sequence, including root-specific elements that may be required for its expression in vascular tissues. Transient expression experiments showed that the Mec1 promoter is functional, since this sequence was able to drive GUS expression in bean embryonic axes. Results from our computational analysis can serve as a guide for functional experiments to identify regions with tissue-specific Mec1 promoter activity. The DNA sequence that we identified is a new promoter that could be a candidate for genetic engineering of cassava roots.

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Expressão transiente do gene gus, sob regulação de quatro promotores, em diferentes tecidos de mamoeiro (Carica papaya L.) e videira (Vitis sp.)

Cited by 2 publications

References 18 publications

Section 7 - Bread Wheat (TRITICUM AESTIVUM)

Section 7 - Bread Wheat (TRITICUM AESTIVUM)

Isolation and characterization of the promoter sequence of a cassava gene coding for Pt2L4, a glutamic acid-rich protein differentially expressed in storage roots

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