In vitro propagation of sacha inchi through organogenesis

Solis, Reynaldo; Cachique, Danter; Guerrero‐Abad, Juan Carlos; Sánchez, María Emilia Ruiz; Figueroa, Lourdes Tapia y

doi:10.1590/s0100-204x2018001100012

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…Conversely, other works have used the apical meristem as an explant for shoot induction. Viegas Rodrigues et al ( 2014) used 1.0 mg L -1 BAP for six weeks of culture and Solis et al (2018) used 0.1 mg L -1 BAP and 0.05 mg L -1 NAA for sprouting 14 days afterwards. This last 14-day response time was similar for this study, where axillary development was observed at 15 days.…”

Section: Discussionmentioning

confidence: 99%

“…Some studies used apical meristems obtained from in vitro plantlets. Viegas Rodrigues et al (2014) achieved shoot formation but did not report the rooting phase and Solis et al (2018) also achieved shoot formation and rooting but did not reach the ex vitro phase. Restrepo-Osorio et al (2020) and Bordignon et al (2012) used different hypocotyl and epicotyl segments of seeds germinated in vitro to obtain plants from induced shoots.…”

Section: Introductionmentioning

confidence: 95%

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In vitro germination and vegetative propagation through bud development of sacha inchi (Plukenetia volubilis L.)

2021

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This study describes the in vitro seed germination and micropropagation of Plukenetia volubilis (sacha inchi), an oilseed crop rich in omega-3 fatty acids, with health benefits and several industrial applications. Seed germination was evaluated in different culture media (MS and 1/2 MS), seed coat presence/absence and culture temperature (18 °C and 28 °C). Micropropagation was performed using axillary bud development (ABD) on nodal segments from in vitro seedlings. KIN, BAP and 2-ip were evaluated for ABD, and the effect of modified MS in 453 mg L-1 CaCl2 and 351.62 mg L-1 MgSO4 on ABD and shoot survival was assessed to improve the process. Finally, six treatments were evaluated to optimize ABD and shoot leaf formation. Seed germination of 91.6 % was achieved in MS at 28 °C when the seed coat was removed. ABD was obtained in 45 % and 40 % with 0.4 mg L-1 KIN and 0.6 mg L-1 2-ip, respectively, with the least CAL. The modification in 453 mg L-1 CaCl2 then allowed 76 % ABD and 82 % explant survival. ABD response was optimized to 95 % and 2.45 leaves with MS medium + CaCl2 modification + 10 % coconut water + 0.4 mg L-1 KIN. The same results were obtained by replacing the latter with 0.6 mg L-1 2-ip. Rooting was achieved in MS without PGR, and acclimatization was successful. The results indicate that plant production via germination and vegetative propagation is effective for commercial purposes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

In vitro germination and vegetative propagation through bud development of sacha inchi (Plukenetia volubilis L.)

2021

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“…Several scientific reports have focused on regeneration from hypocotyls and epicotyls in the Euphorbiaceae family [ 5 , 6 ], despite high recalcitrance. Regarding sacha inchi specifically, a few studies have reported the use of in vitro propagation to evaluate the auxin:cytokinin ratio in apical meristems [ 7 ] and different segments of the epicotyl and hypocotyl [ 8 – 10 ], with highest shooting but low process efficiency with 0.5 and 1 mg/L 6-benzylaminopurine and 0.1 mg/L and 0.25 mg/L 1-naphthylacetic acid (NAA). Therefore, it is imperative that scientific knowledge regarding in vitro establishment and effective propagation methods for this promising species is expanded as demand for its oilseed has recently increased worldwide.…”

Section: Introductionmentioning

confidence: 99%

Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)

et al. 2020

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Objective It is necessary to improve biotech platforms based on in vitro cell tissue culture to support sacha inchi ( Plukenetia volubilis L.) research programs and draw on the nutritional value of the high polyunsaturated fatty acid content of its oilseed. Here, we developed a rapid and efficient method for induction and direct in vitro shoot development for this species. Results Shoots were generated from hypocotyl explants. The highest organogenic response was obtained in woody plant medium supplemented with 1 mg/L thidiazuron and 0.5 mg/L zeatin supplemented with L-glutamine, adenine hemisulfate, and L-arginine. Shoots obtained using this medium were transferred and subcultivated with different concentrations of indole-3-butyric acid and 1-naphthylacetic acid for rooting. For the first time, a histological analysis was performed supporting direct organogenic development in this species. The plantlets obtained were transferred ex vitro with a survival percentage of 80%. The genetic stability of the plants recovered was confirmed by randomly amplified polymorphic DNA analysis. All results indicate that it would be possible to stimulate direct shoot formation from hypocotyls to support the sustainable use of this species.

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Rooting of sacha inchi (Plukenetia volubilis) juvenile cuttings in microtunnels

et al. 2019

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The seeds of sacha inchi (Plukenetia volubilis) have high levels of proteins and unsaturated fatty acids, and due to their nutraceutical potential, their demand in the international market has increased in recent years. This study aims at developing a method for rooting juvenile cuttings of sacha inchi in microtunnels, in order to propagate plants with superior genetic traits and shorten production cycles. Two experiments were carried out in randomized complete block designs. In the first were used juvenile cuttings of 8 cm and 2000 ppm of IBA according to previos studies, and tested two types of substrates (sand and Jiffy pellets) and two frequencies of nebulized irrigation (once per day and three times per day, each application was for 30 seconds). In the second experiment were used again cuttings of 8 cm, and tested three levels of leaf area (25, 50 and 75 cm2) and five concentrations of indolebutyric acid (0, 1000, 2000, 4000 and 6000 ppm). Data were submitted to analysis of variance and means were compared by Tukey test at 5% probability. The results show that using Jiffy pellets, one nebulized irrigation per day, juvenile cuttings of 8 cm length with 75 cm2 of leaf area, and 2000 ppm of indolebutyric acid induced high percentages of rooting (93.3%) and the best root formation in the process of vegetative propagation of sacha inchi.

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In vitro propagation of sacha inchi through organogenesis

Cited by 4 publications

References 14 publications

In vitro germination and vegetative propagation through bud development of sacha inchi (Plukenetia volubilis L.)

In vitro germination and vegetative propagation through bud development of sacha inchi (Plukenetia volubilis L.)

Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)

Rooting of sacha inchi (Plukenetia volubilis) juvenile cuttings in microtunnels

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