Desenvolvimento in vitro de bananeira 'Ouro' após poliploidização com antimitóticos

Borges, Viviane Peixoto; Marques, Thiago de Santana; Reis, Alda Silva dos; Oliveira, Neuza Helena Carvalho de; Jesus, Jamily Almeida de; Silveira, Daniela Garcia; Santos-Serejo, Janay Almeida dos; Silva, Sebastião de Oliveira e; Lêdo, Carlos Alberto da Silva

doi:10.1590/s0100-204x2016001000011

Cited by 4 publications

(2 citation statements)

References 9 publications

(10 reference statements)

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“…Autotetraploid plants can be obtained by crossing triploids with diploids, chromosome doubling of diploids, somatic hybridization, or genetic engineerin (Do Amaral et al, 2015;Borges et al, 2016;De Carvalho Santos et al, 2019). Induction of chromosome doubling using colchicine is widely used in polyploid mutagenesis of various plants.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and metabolome profiling provide insights into hormone-mediated enhanced growth in autotetraploid seedlings of banana (Musa spp.)

Zhan

Li²,

Zhang³

et al. 2023

Front. Sustain. Food Syst.

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IntroductionReconstructive breeding based on autotetraploids to generate triploid varieties is a promising breeding strategy in banana (Musa spp.). Therefore understanding the molecular mechanisms underlying the phenotypic differences between the original diploid and its autopolyploid derivatives is of significant importance in such breeding programs of banana.MethodsIn this study, a number of non-chimeric autotetraploid plants, confirmed by flow cytometry and chromosome counting were obtained using colchicine treatment of ‘Pisang Berlin' (AA Group), a diploid banana cultivar highly resistant to Fusarium wilt Tropical Race 4 (Foc TR4) and widely cultivated in Asia.Results and discussionThe autotetraploids showed significant increase in plant height, pseudostem diameter, root length, leaf thickness, leaf area, and leaf chlorophyll content. Transcriptomic analysis indicated that differentially expressed genes were mainly enriched in plant hormone signal transduction, mitogen-activated protein kinase (MAPK) signaling pathway, and carbon fixation in photosynthetic organelles. The genes related to the metabolism, transport or signaling of auxin, abscisic acid (ABA), cytokinin (CTK) and gibberellin (GA), as well as the genes encoding essential enzymes in photosynthetic CO2 fixation were differentially expressed in leaves of autotetraploids and most of them were up-regulated. Metabolomic analysis revealed that the differentially accumulated metabolites were mainly involved in plant hormone signal transduction, porphyrin and chlorophyll metabolism, indole alkaloid biosynthesis, and carbon fixation in photosynthetic organelles. The results therefore, demonstrate that the hormones IAA, ABA, and photosynthetic regulation may play a vital role in the observed enhancement in the autotetraploids. These could be used as molecular and biochemical markers to facilitate the generation of triploid progenies as suitable new varieties for cultivation.

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Section: Introductionmentioning

confidence: 99%

Transcriptome and metabolome profiling provide insights into hormone-mediated enhanced growth in autotetraploid seedlings of banana (Musa spp.)

Zhan

Li²,

Zhang³

et al. 2023

Front. Sustain. Food Syst.

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“…The use of biotechnology tools has allowed advances in banana genetic studies, especially through techniques such as genetic engineering, induction of mutations, somaclonal variation, somatic hybridization, and polyploidy, which can be used to overcome the barriers of natural sterility found in some cultivars ( Pestana et al, 2011 ; Amaral et al, 2015 ; Borges et al, 2016 ; Rocha et al, 2021 ). Among the mentioned tools the induction of somaclonal variations has been recognized as important for generating genetic variability as an alternative for banana genetic improvement, which allows the selection of somaclones with the desired characteristics ( Ghag et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Pre-selection of banana somaclones resistant to Fusarium oxysporum f. sp. cubense, subtropical race 4

et al. 2021

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– Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), is one of the most destructive diseases affecting banana crops worldwide. Therefore, the development of resistant cultivars is a promising alternative to mitigate the effects of the disease on banana plantations. The objectives of this study were to induce somaclonal variation in banana cultivars of the Silk and Cavendish types and to select somaclones resistant to subtropical race 4, thereby enabling the production of fruit in areas where this race is present. Shoot clump apexes of the Grand Naine and Maçã (Silk) cultivars were grown in MS medium. The cultures were subcultured four times. They were then challenged with fusaric acid (FA) in an experiment consisting of four treatments with different concentrations (0.1, 0.2, 0.3, and 0.4 mM) and five repetitions, each consisting of a Petri dish containing seven multiple shoot clumps in MS culture medium supplemented with 2.5 mg/L benzylamine purine. Multiple shoot clumps without the addition of FA were also used in the experiment, and were subcultured three times and maintained in a dark room. The multiple shoot clumps that survived the treatment with FA were transferred to MS medium and maintained in the growth chamber in the presence of light. The regenerated plants were later planted in tanks containing soil infested with an isolate classified as Foc subtropical race 4 (Foc STR4), and were evaluated for resistance to the pathogen at 90 days after inoculation (d.a.i.). Pathogen structures were confirmed by root clarification and root staining technique. All somaclones of the Maçã (Silk) cultivar were susceptible to Fusarium wilt and two somaclones of the Grand Naine cultivar were selected as resistant. The addition of FA as a selective agent was effective in the selection of somaclones among plants of the Grand Naine cultivar, as shown by the selection of two somaclones resistant to Foc STR4. The next step will consist of the agronomic and market potential validation of the selected somaclones, aiming to confirm their potential use by producers.

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Genetic variability among autotetraploid populations of banana plants derived from wild diploids through chromosome doubling using SSR and molecular markers based on retrotransposons

et al. 2019

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Desenvolvimento in vitro de bananeira 'Ouro' após poliploidização com antimitóticos

Cited by 4 publications

References 9 publications

Transcriptome and metabolome profiling provide insights into hormone-mediated enhanced growth in autotetraploid seedlings of banana (Musa spp.)

Transcriptome and metabolome profiling provide insights into hormone-mediated enhanced growth in autotetraploid seedlings of banana (Musa spp.)

Pre-selection of banana somaclones resistant to Fusarium oxysporum f. sp. cubense, subtropical race 4

Genetic variability among autotetraploid populations of banana plants derived from wild diploids through chromosome doubling using SSR and molecular markers based on retrotransposons

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