Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil

Rivas, Eliana Borges; Bôdi, Estevão Cardoso de Almeida; Harakava, Ricardo; Gregori, Fábio; Gonçalves, Marcos César

doi:10.1590/s0100-204x2016000500023

Cited by 3 publications

(5 citation statements)

References 23 publications

(50 reference statements)

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“…The two subgroups (“Tulip breaking virus lily strain” and “Tulip band breaking virus”) of LMoV were described previously by Zheng et al [ 32 ]. However these subgroup designations are referring to obsolete species names, we provide evidence to support the findings of Rivas et al [ 33 ] who renamed the Tulip band breaking virus group to subgroup I and Tulip breaking virus lily strain group to subgroup II of Lily mottle virus. Interestingly, all of the LMoV isolates identified in this study from tulips grouped into the LMoV subgroup I.…”

Section: Resultssupporting

confidence: 89%

Genetic Diversity of Potyviruses Associated with Tulip Breaking Syndrome

Ágoston

Almási

Salánki

2020

Plants

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Tulip breaking is economically the most important viral disease of modern-day tulip growing. It is characterized by irregular flame and feather-like patterns in the flowers and mosaic on the foliage. Thirty-two leaf samples were collected from cultivated tulip plants showing tulip breaking syndrome from Hungary in 2017 and 2018. Virus identification was performed by serological (ELISA) and molecular (RT-PCR) methods. All samples proved to be infected with a potyvirus and evidence was provided that three potyvirus species could be identified in the samples: Lily mottle virus (LMoV), Tulip breaking virus (TBV) and Rembrandt tulip-breaking virus (ReTBV). Recombination prediction accomplished with Recombination Detection Program (RDP) v4.98 revealed potential intraspecies recombination in the case of TBV and LMoV. Phylogenetic analyses of the coat protein (CP) regions proved the monophyletic origin of these viruses and verified them as three different species according to current International Committee on Taxonomy of Viruses (ICTV) species demarcation criteria. Based on these results, we analyzed taxonomic relations concerning potyviruses associated with tulip breaking syndrome. We propose the elevation of ReTBV to species level, and emergence of two new subgroups in ReTBV.

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Section: Resultssupporting

confidence: 89%

Genetic Diversity of Potyviruses Associated with Tulip Breaking Syndrome

Ágoston

Almási

Salánki

2020

Plants

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“…Of them, viruses infecting lily species cause serious damage to the quality and quantity of lily production. Since the first report of the three major viruses infecting the lily in 1944 (i.e., Lily symptomless virus (LSV) in the genus Carlavirus , Lily mottle virus (LMoV) in the genus Potyvirus , and Cucumber mosaic virus (CMV) in the genus Cucumovirus [ 6 ]), more than 20 viruses infecting the lily have been reported [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

RNA viromes of the oriental hybrid lily cultivar “Sorbonne”

Cho

2018

BMC Genomics

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BackgroundThe lily is a perennial flowering plant belonging to the genus Lilium in the family Liliaceae. Most cultivated lily plants are propagated by bulbs. Therefore, numerous lily bulbs are frequently infected by diverse viruses causing viral diseases. To date, no study has examined the viromes of plants of one type with identical genetic backgrounds collected from different geographical regions.ResultsHere, we examined different viromes of the lily cultivar “Sorbonne” using 172 gigabytes of transcriptome data composed of 23 libraries from four different projects for the cultivar “Sorbonne.” We identified 396 virus-associated contigs from all but one library. We identified six different viruses, including Plantago asiatica mosaic virus (PlAMV), Cucumber mosaic virus (CMV), Lily symptomless virus (LSV), Tulip virus X (TVX), Lily mottle virus (LMoV), and Tobacco rattle virus (TRV). Of them, PlAMV was the most common virus infecting the lily. Scale and flower samples possessed a high number of virus-associated reads. We assembled 32 nearly complete genomes for the six identified viruses possessing the polyadenylate tails. Genomes of all six viruses were highly conserved in the lily cultivar “Sorbonne” based on mutation analysis. We identified defective RNAs from LSV, TVX, and PlAMV localized in the triple gene block region. Phylogenetic analyses showed that virus genomes are highly correlated with geographical regions and host plants.ConclusionsWe conducted comprehensive virome analyses of a single lily cultivar, “Sorbonne,” using transcriptome data. Our results shed light on an array of lily virome-associated topics, including virus identification, the dominant virus, virus accumulation in different plant tissues, virus genome assembly, virus mutation, identification of defective RNAs, and phylogenetic relationships of identified viruses. Taken together, we provide very useful methods and valuable results that can be applied in other virome-associated studies.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5138-3) contains supplementary material, which is available to authorized users.

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“…LSV and its co-infections with CMV or LMoV have been reported in various endemic regions worldwide. Some of the regions where these viruses have been identified include: the Netherlands, China, Japan, and the United States [21,27,98]. Mixed infection with these viruses leads to severe symptoms like smaller flowers, lower bulb yield, and growth reduction.…”

Section: Reverse Transcription Loop-mediated Isothermal Amplificationmentioning

confidence: 99%

“…Consequently, the ID-ELISA method and IC-RT-PCR assays have been developed for efficient lily virus detection [104]. These methods were recommended for further control, surveys, and also to prevent lily infection by most of the viruses that occur in Lanzhou/Gansu Province and NingXia, the most important lily production areas in China [21,98]. Next-generation sequencing: This is a high-throughput sequencing technique that can detect and identify viral sequences in a sample.…”

Section: Generation and Purification Of Recombinant Coat Proteinsmentioning

confidence: 99%

“…The aim is to establish an economically viable approach for lily plant production [5]. While it is important to acknowledge the significant progress made in reducing the incidence of major viruses in planting stock through advanced virus detection techniques such as immunoassay and molecular methods, it is essential to recognize that detection alone is insufficient in minimizing the presence of viruses [21]. To achieve effective virus control, a comprehensive approach encompassing both detection and elimination strategies is required.…”

Section: Introductionmentioning

confidence: 99%

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Advancements in Lily Viruses Management: Challenges and Solutions in Elimination and Detection

Gong

Dusengemungu

et al. 2023

Horticulturae

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Lilies are important crops that are commonly used as cut flowers (Lilium spp.) and edible bulb crops (Lilium davidii var. unicolor). However, virus infections can significantly impact the quantity and quality of lily production. Various methods have been developed to eliminate viruses in lilies, including in vitro culture and virus detection techniques. Meristem culture is the most effective method, which can be combined with other techniques such as thermotherapy and chemotherapy. Nonetheless, virus elimination is affected by several factors, including cultivar, explants used, virus type, and duration of treatments. Efficient diagnostic methods, such as serological and molecular techniques, have been developed to detect viral infections in lilies, including enzyme-linked immunosorbent assay (ELISA) and real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR). However, cross-contamination and multiple-virus contamination can lead to unreliable results, and more sophisticated protocols and systems have been developed to address these issues. The objective of this review is to provide a comprehensive overview of the development of lily virus eradication, detection strategies, challenges, and solutions associated with these procedures, and how more sophisticated approaches such as multiplex RT-PCR, indirect ELISA (ID-ELISA), immunocapture RT-PCR (IC-RT-PCR), and immunochromatographic test strips (ICSs) can alleviate some of these setbacks.

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Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil

Cited by 3 publications

References 23 publications

Genetic Diversity of Potyviruses Associated with Tulip Breaking Syndrome

Genetic Diversity of Potyviruses Associated with Tulip Breaking Syndrome

RNA viromes of the oriental hybrid lily cultivar “Sorbonne”

Advancements in Lily Viruses Management: Challenges and Solutions in Elimination and Detection

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