In vitro maintenance, under slow-growth conditions, of oil palm germplasm obtained by embryo rescue

Camillo, Julcéia; Scherwinski‐Pereira, Jonny Everson

doi:10.1590/s0100-204x2015000500010

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…In embryogenic cultures of Phoenix dactylifera, addition of 4.0% or 6.0% mannitol and 2.0% sorbitol showed the highest percentage of survival in Bartamoda cultivar (El-Bahr et al, 2016). However, in another Arecaceae species, Elaeis guineensis and E. oleifera, mannitol at 1.0, 2.0, and 3.0% after 12 months, the temperature of 20 o C reduced both plant growth and plant survival (Camillo and Scherwinski-Pereira, 2015).…”

Section: Conflict Of Interestmentioning

confidence: 95%

Plant Tissue Culture in Muntingia Calabura in Vitro Clonal Propagation, Callus Induction and Germplasm Conservation of ‘Cerecillo’ Muntingia Calabura L. (Muntingiaceae)

Yohonatan

Rojas-Idrogo

Guillermo

2020

Int. J. Res. Granthaalayah

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Muntingia calabura L. (Muntingiaceae) is a fast-growing tree native to tropical America, abundant in the seasonally dry forest of the north coast of Peru. Tissue culture is an effective procedure to produce healthy plants, rapid clonal propagation and several morphogenic process. The objective of this study was to formulate an efficient method for micropropagation, morphogenesis callus induction and germplasm conservation of this species. In this study, seeds, shoot-tips and nodal segments of seedlings were used as explants, inducing various morphogenic processes in different combinations of growth regulators and osmoregulatory substances. In vitro seeds germination was 100% up to four months after the ripe fruits were collected and after 12 months the germination rate was 0.0%. The highest elongation of shoot was observed with 0.5 mg L-1 2iP (3.11 cm) although the highest number of shoots formed (18.0 and 16.5) was observed with 0.5 mg/L KIN or TDZ, respectively, after 30 days of culture. The best callus induction was obtained in 0.5 and 1.0 mg L-1 TDZ, 1.0 and 2.0 mg L-1 2,4-D, 2.0 mg L-1 NAA or 2.0 mg L-1 NAA with 0.1 and 0.5 mg L-1 BAP, after 45 days of culture period. Shoot regeneration (> 10 shoots/explant) was observed with 0.1 to 2.0 mg L-1 NAA. Root induction was observed in all shoots cultured in various concentrations of IBA and NAA-GA3, after 30 days of culture. After two months, well rooted plantlets were transplanted in greenhouse conditions, however, the survival rate was less than 10%. Only in treatment with mannitol 2.0% in explants without roots, the highest in vitro conservation rate (50%) was reached, after 6 months of culture, while in the control treatment in culture medium without ABA and mannitol, but supplemented with 0.02-0.02 mg L-1 (IAA-GA3), the conservation rate reached 100%. The results demonstrated the applicability of tissue culture in the micropropagation and in vitro germplasm conservation of M. calabura.

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Section: Conflict Of Interestmentioning

confidence: 95%

Plant Tissue Culture in Muntingia Calabura in Vitro Clonal Propagation, Callus Induction and Germplasm Conservation of ‘Cerecillo’ Muntingia Calabura L. (Muntingiaceae)

Yohonatan

Rojas-Idrogo

Guillermo

2020

Int. J. Res. Granthaalayah

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“…Hal ini bertujuan agar tanaman tersebut dapat disimpan dalam jangka waktu lama [9]. Penelitian terkait konservasi in vitro dengan penerapan teknik slow growth telah dilakukan untuk tanaman kelapa sawit (Elaeis guineensis dan E. oleifera) [10], Senecio macrophyllus [11], anggrek endemik di Meksiko Laelia anceps Lindl. [12], Vanilla planifoliaJacks [13], tanaman obat Dioscorea floribunda [14] dan Amburana cearensis [15].…”

Section: Pendahuluanunclassified

Konservasi In Vitro Kantong Semar (Nepenthes rafflesiana Jack.) dengan Metode Slow Growth

Previaningrum

Qadir

Isnaini

2021

JJMS

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Nepenthes rafflesiana Jack. merupakan tumbuhan unik karena ujung daunnya yang memiliki sulur dengan membentuk kantong untuk merangkap serangga. Keunikannya ini membuat N. rafflesiana banyak diambil dari habitat aslinya untuk dijual. Pengambilan dari alam dapat menyebabkan kepunahan dan kerusakan habitat ekosistem. Upaya mencegah kepunahan melalui konservasi ex situ dengan kultur N. rafflesiana secara in vitro dengan metode slow growth. Penelitian ini bertujuan untuk mengetahui pengaruh paclobutrazol terhadap pertumbuhan N. rafflesiana dan untuk mendapatkan konsentrasi paclobutrazol yang paling sesuai dalam konservasi in vitro N. rafflesiana. Penelitian dilakukan di Laboratorium Kultur Jaringan Pusat Penelitian Konservasi Tumbuhan dan Kebun Raya dari bulan Januari sampai dengan April 2020. Rancangan yang dilakukan dalam percobaan adalah Rancangan Kelompok Lengkap Teracak dengan faktor tunggal yaitu konsentrasi paclobutrazol: 0, 1, 3 dan 5 ppm. Hasil penelitian menunjukkan konsentrasi paclobutrazol terbaik untuk konservasi in vitro N. rafflesiana adalah 5 ppm yang dapat menghambat pertumbuhan tinggi batang dan jumlah daun serta dapat mempertahankan warna hijau daun.

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“…On the other hand, PEG (higher than 3000 MW) cannot pass through the apoplastic barrier of cells—therefore, its osmotic stress is less pronounced, especially in the leaves [ 4 ]. Mannitol is a polyhydric alcohol, and the addition of 50–200 mM of mannitol into the culture media decreased growth and increased the sub-culturing period in many crops [ 5 , 6 , 7 ]. A higher concentration of mannitol reduced the water potential of the nutrient medium and made the transition of water into the plant tissues more difficult causing the osmotic stress [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Biochemical and Genetic Responses of Tea (Camellia sinensis (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro

Самарина

Matskiv

Simonyan

et al. 2020

Plants

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Osmotic stress is a major factor reducing the growth and yield of many horticultural crops worldwide. To reveal reliable markers of tolerant genotypes, we need a comprehensive understanding of the responsive mechanisms in crops. In vitro stress induction can be an efficient tool to study the mechanisms of responses in plants to help gain a better understanding of the physiological and genetic responses of plant tissues against each stress factor. In the present study, the osmotic stress was induced by addition of mannitol into the culture media to reveal biochemical and genetic responses of tea microplants. The contents of proline, threonine, epigallocatechin, and epigallocatechin gallate were increased in leaves during mannitol treatment. The expression level of several genes, namely DHN2, LOX1, LOX6, BAM, SUS1, TPS11, RS1, RS2, and SnRK1.3, was elevated by 2–10 times under mannitol-induced osmotic stress, while the expression of many other stress-related genes was not changed significantly. Surprisingly, down-regulation of the following genes, viz. bHLH12, bHLH7, bHLH21, bHLH43, CBF1, WRKY2, SWEET1, SWEET2, SWEET3, INV5, and LOX7, was observed. During this study, two major groups of highly correlated genes were observed. The first group included seven genes, namely CBF1, DHN3, HXK2,SnRK1.1, SPS, SWEET3, and SWEET1. The second group comprised eight genes, viz. DHN2, SnRK1.3, HXK3, RS1, RS2,LOX6, SUS4, and BAM5. A high level of correlation indicates the high strength connection of the genes which can be co-expressed or can be linked to the joint regulons. The present study demonstrates that tea plants develop several adaptations to cope under osmotic stress in vitro; however, some important stress-related genes were silent or downregulated in microplants.

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In vitro maintenance, under slow-growth conditions, of oil palm germplasm obtained by embryo rescue

Cited by 3 publications

References 11 publications

Plant Tissue Culture in Muntingia Calabura in Vitro Clonal Propagation, Callus Induction and Germplasm Conservation of ‘Cerecillo’ Muntingia Calabura L. (Muntingiaceae)

Plant Tissue Culture in Muntingia Calabura in Vitro Clonal Propagation, Callus Induction and Germplasm Conservation of ‘Cerecillo’ Muntingia Calabura L. (Muntingiaceae)

Konservasi In Vitro Kantong Semar (Nepenthes rafflesiana Jack.) dengan Metode Slow Growth

Biochemical and Genetic Responses of Tea (Camellia sinensis (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro

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