Carotenogênese em células de Haematococcus pluvialis induzidas pelos estresses luminoso e nutricional

Abstract

Resumo -O objetivo deste trabalho foi avaliar as respostas das células de Haematococcus pluvialis ao processo de indução à carotenogênese, sob estresse luminoso e nutricional. As células foram aclimatadas durante 15 dias em meio WC, com aeração com ar atmosférico sintético filtrado e fluxo de 100 mL min ; e cultivo nas mesmas condições do tratamento anterior, mas com aeração contendo 4% de CO 2 . Os tratamentos foram conduzidos em triplicata, durante dez dias. Com a adição de CO 2 e o incremento da iluminação, observou-se aumento da razão carotenoides/clorofila e da biomassa celular. As células cessaram a divisão no segundo dia de estresse, quando o nitrato se tornou limitante, e aumentaram significativamente seu biovolume. A excreção de carbono orgânico e a concentração de astaxantina aumentam em resposta à adição de CO 2 . O estresse por intensidade luminosa, aliado à adição de CO 2 , otimiza a carotenogênese em H. pluvialis e aumenta a produção de astaxantina.Termos para indexação: astaxantina, carbono orgânico dissolvido, carotenoide, intensidade luminosa, microalga. Carotenogenesis in Haematococcus pluvialis cells induced by light and nutrient stressesAbstract -The objective of this work was to evaluate the responses of Haematococcus pluvialis cells to the carotenogenesis induction process, under light and nutrition stress. Cells were acclimated during 15 days in WC medium, with aeration with synthetic, filtered atmospheric air and flow rate of 100 mL min , photoperiod of 12 hours, and temperature of 23°C. The following two treatments were compared: cultivation under the described conditions, but with increase of light intensity up to 350 µmol photons m -2 s -1 ; and cultivation under the same conditions as the previous treatment, but with aeration containing 4% CO 2 . The treatments were done in triplicate, during ten days. With the addition of CO 2 and the increment in lighting, an increase was observed in the carotenoids/chlorophyll ratio and cell biomass. Cells stopped dividing on the second day of stress, when nitrate became limiting, and significantly increased their biovolume. The excretion of organic carbon and the concentration of astaxanthin increase in response to the addition of CO 2. Stress by light intensity combined with CO 2 addition optimizes carotenogenesis in H. pluvialis and increases astaxanthin production.

Desmistificação De Mitos Botânicos Por Meio Do Estudo Da Fisiologia De Plantas

“…: temperatura, água, luminosidade etc.) (Ferraz et al, 2006;Lucena et al, 2013;Nunes et al, 2013), para garantir sua sobrevivência. De todo modo, ao elencarmos as ondas sonoras como possíveis agentes estressantes, os vegetais também necessitarão expressar características metabólicas para enfrentar esse suposto estresse.…”

Extraction of Valuable Biomolecules from the Microalga Haematococcus pluvialis Assisted by Electrotechnologies

“…The recent literature on cultivation and growth conditions of H. pluvialis is rather vast. It includes discussions of the effects of temperature [11], pH [7], nutrient concentration (nitrogen, phosphorus and carbon starvation) [6,33] and illumination conditions [8][9][10][34][35][36]. The effects of light illumination on the growth of biomass and accumulation of bioastaxanthin were analyzed, and it was demonstrated that red light is more effective for increasing the biomass, whereas blue light supports the accumulation of bioastaxanthin [10].…”

Advances in the Biotechnological Potential of Brazilian Marine Microalgae and Cyanobacteria

“…Nunes and collaborators [ 110 ] showed that astaxanthin content was changed depending on the stimulus given to H. pluvialis (which was 4.2 times higher), and these products are found in the form of powder concentrates, lyophilized or dehydrated biomass, as well as extracts in vegetable oil and pigments that are very often applied in the food market [ 111 ].…”