Patogenicidade de Beauveria bassiana ao psilídeo Diaphorina citri e compatibilidade do fungo com produtos fitossanitários

Abstract

Resumo -O objetivo deste trabalho foi avaliar a patogenicidade de Beauveria bassiana a ninfas de Diaphorina citri (Hemiptera: Psyllidae) e verificar a compatibilidade do fungo com produtos fitossanitários e sua persistência em plantas de citros. Termos para indexação: controle biológico, fungos entomopatogênicos, greening, "huanglongbing", psilídeo-dos-citros. Beauveria bassiana pathogenicity to Diaphorina citri and compatibility of the fungus with phytosanitary productsAbstract -The objective of this work was to evaluate the pathogenicity of Beauveria bassiana to Diaphorina citri (Hemiptera: Psyllidae) nymphs, and to check the compatibility of the fungus with phytosanitary products, and its persistence in citrus plants. Nymphs of D. citri were sprayed with B. bassiana in the concentrations 5x10 6 , 1x10 7 , 5x10 7 , 1x10 8 , 5x10 8 and 1x10 9 conidia mL -1 for lethal concentration determination. In order to evaluate the compatibility of the phytosanitary products with the fungus, neem extract and five insecticides of four different chemical groups were individually added to PDA culture medium, in which the fungus was cultivated. Vegetative growth, sporulation and viability of the entomopathogen were evaluated. In the greenhouse, citrus plants were first treated with the phytosanitary products and, then, with the pathogen. Exposure times of 24 hours, 7 and 14 days were evaluated. Fungus pathogenicity to D. citri nymphs was confirmed; LC50 of 0.4 x10 7 and LC90 of 6.7 x10 7 conidia mL -1 were determined at the tenth day of evaluation. In the laboratory, the phytosanitary products reduced fungus growth. In greenhouse, the products did not affect fungus survival on citrus plants.

Use of the entomopathogenic fungi Beauveria bassiana (Hyphomycetes: Moniliales) and Isaria fumosorosea (Hypocreales: Cordycipitaceae) to control Diaphorina citri Kuwayama (Hemiptera: Liviidae) under laboratory and semi-field conditions

“…I. fumosorosea induced (9.4-10.9%) higher mortality rate of psyllid adults under laboratory and semi-field conditions. The efficacy of fungal species against ACP has been reported earlier (Hoy et al 2010;Ferreira Pinto et al 2012;Stauderman et al 2012). The results are in accordance to those of Ferreira Pinto et al (2012), Lezama-Gutiérrez et al (2012, and Ausique et al (2017).…”

“…The efficacy of fungal species against ACP has been reported earlier (Hoy et al 2010;Ferreira Pinto et al 2012;Stauderman et al 2012). The results are in accordance to those of Ferreira Pinto et al (2012), Lezama-Gutiérrez et al (2012, and Ausique et al (2017). The entomopathogens cause the direct effect on insect pests by infection; however, Avery et al (2011) reported that I. fumosorosea decreased the feeding activity of ACP before their death and led to a reduction in the transmission of the bacterial pathogen responsible for citrus greening disease in citrus.…”

Transmission potential of the entomopathogenic fungi Isaria fumosorosea and Beauveria bassiana from sporulated cadavers of Diaphorina citri and Toxoptera citricida to uninfected D. citri adults

“…Entomopathogenic fungi are important natural enemies of pests because they infect a large number of arthropods, and under favorable conditions, they can initiate epizootics in the field (Alves 1998). The pathogenicity of certain fungal species to D. citri has been reported, such as Isaria fumosorosea, Beauveria bassiana, Hirsutella citriformis, Lecanicillium lecanii, L. muscarium, L. longisporum, Metarhizium anisopliae and M. brunneum (Ascomycota: Hypocreales) (Avery et al 2009;Casique-Valdes et al 2011;Gandarilla-Pacheco et al 2013;Hall et al 2012;Hoy et al 2010;Hunter et al 2011;Lezama-Gutiérrez et al 2012;Meyer et al 2007Meyer et al , 2008Padulla and Alves 2009;Pinto et al 2012;Stauderman et al 2012;Subandiyah et al 2000;Yang et al 2006). The entomopathogenic fungi I. fumosorosea, B. bassiana, L. lecanii and M. anisopliae have been reported as pathogens of T. citricida (Balfour and Khan 2012;Hernández-Torres et al 2006;Pick et al 2012;Poprawski et al 1999).…”

Respuesta de Diaphorina citri1 Kuwayama a Cepas de Hongos Entomopatógenos en Argentina

“…Estudios de laboratorio sobre la virulencia de B. bassiana , Metarhizium anisopliae (Mestschikoff) Sorokin e I . fumosorosea en ninfas y adultos de D. citri (Padulla y Alves 2009, Avery et al 2011, Ferreyra Pinto et al 2012, Conseschi 2013, Saldarriaga Ausique et al 2017), registraron niveles de mortalidad del 40 al 100% mientras que en campo, estudios de efectividad biológica de M. anisopliae , B. bassiana , I. fumosorosea e H. citriformis contra ninfas y adultos de D. citri han sido desarrollados por Mellín-Rosas et al (2011), Lezama-Gutiérrez et al (2012, 2014), Saldarriaga Ausique et al (2017) y Pérez-González et al . (2020) con resultados variables.…”