Tolerância de sementes de dendezeiro à criopreservação

Camillo, Julcéia; Luis, Zanderluce Gomes; Scherwinski‐Pereira, Jonny Everson

doi:10.1590/s0100-204x2009000200015

Cited by 13 publications

(13 citation statements)

References 4 publications

(5 reference statements)

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“…Nunes et al (2008) observaram que a maior e a menor concentração de sacarose no meio de cultura afetam a produção de raízes de plântulas de pinhão-manso oriundas de sementes germinadas in vitro. Camillo et al (2009), em trabalho com criopreservação de sementes de dendezeiro (Elaeis guineensis), obtiveram taxa de germinação de 90%, após 21 dias de crioarmazenamento. Esses autores observaram raízes primárias bem desenvolvidas e ausência de plântulas anormais ao final da avaliação, o que mostra o potencial das técnicas de criopreservação na conservação de sementes de certos genótipos de dendezeiro, sem comprometer sua viabilidade.…”

Section: Resultsunclassified

Potencial germinativo e morfoanatomia foliar de plântulas de pinhão-manso originadas de germoplasma criopreservado

Silva

Camillo

Luis

et al. 2011

Pesq. agropec. bras.

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O objetivo deste trabalho foi avaliar o potencial germinativo e caracterizar morfoanatomicamente a estrutura foliar de plântulas de pinhão-manso originadas de germoplasma criopreservado. Inicialmente, as sementes foram avaliadas quanto à dessecação, a cada 24 horas, por até 120 horas. Posteriormente, as sementes foram dessecadas por 0, 24 e 48 horas e avaliadas mensalmente quanto ao potencial germinativo, antes e após armazenamento em nitrogênio líquido por imersão direta e congelamento rápido. Para caracterizar a viabilidade das sementes que não germinaram após o período de avaliação, os embriões zigóticos foram imersos em solução de tetrazólio. A morfoanatomia foliar após criopreservação foi caracterizada por meio de estudos histológicos. As sementes de pinhão-manso toleraram a dessecação a níveis críticos e a criopreservação com umidade em torno de 8%. A dessecação das sementes até valores baixos, associada a períodos prolongados de exposição ao nitrogênio líquido, causa anormalidade de plântulas, danifica as células e os tecidos das folhas, e afeta negativamente a germinação.

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Section: Resultsunclassified

Potencial germinativo e morfoanatomia foliar de plântulas de pinhão-manso originadas de germoplasma criopreservado

Silva

Camillo

Luis

et al. 2011

Pesq. agropec. bras.

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“…Consequently, all sources of seed deterioration that are metabolically related are greatly reduced or stopped, thus providing 'indefinite' preservation. Storage in LN could prevent or significantly reduce such viability loss (Camillo et al 2009). Thus, the result would be the improved maintenance of valuable genetic resources.…”

Section: Discussionmentioning

confidence: 99%

A method for seedling recovery in <i>Jatropha curcas</i> after cryogenic exposure of the seeds.

Silva

Camillo

Scherwinski‐Pereira

2012

RBT

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Actually, the germplasm of Jatropha spp. is conserved as whole plants in field collections. Under this storage method, the genetic resources are exposed to disease, pest and natural hazards such as human error, drought and weather damage. Besides, field genebanks are costly to maintain and with important requirements of trained personnel. Thus, the development of efficient techniques to ensure its safe conservation and regeneration is therefore of paramount importance. In this work we describe a method for Jatropha curcas seeds cryoexposure and seedling recovery after thawed. In a first experiment, an efficient protocol for in vitro plant recovery was carried out using zygotic embryo or seeds with or without coat. In a second experiment, desiccated seeds with or without coat were exposed to liquid nitrogen and evaluated after cryoexposure. Germination percentages were variable among treatments, and seeds demonstrated tolerance to liquid nitrogen exposure under certain conditions. Seeds of J. curcas presented up to 99.6% germination after seed coat removal. Seeds with coat cultured in vitro did not germinate, and were 60% contaminated. The germination of the zygotic embryos was significantly higher in the ½ MS medium (93.1%) than in WPM medium (76.2%), but from zygotic embryo, abnormal seedlings reached up to 99%. Seeds with coat exposed to liquid nitrogen showed 60% germination in culture after coat removal with good plant growth, and seeds cryopreserved without coat presented 82% germination, but seedlings showed a reduced vigor and a significant increase in abnormal plants. Seeds cultured in vitro with coat did not germinate, independently of cryoexposure or not. This study reports the first successful in vitro seedling recovery methodology for Jatropha curcas seeds, after a cryopreservation treatment, and is recommended as an efficient procedure for in vitro plant recovery, when seeds are conserved in germplasm banks by low or cryotemperatures. Rev. Biol. Trop. 60 (1): 473-482. Epub 2012 March 01.

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“…According to Camillo et al (2009a), maintenance of field genebanks is expensive and new strategies are needed. Oil palm seeds are intermediate according to Ellis et al (1991).…”

Section: Cryopreservation Of Economically Important Non-native and Namentioning

confidence: 99%

“…Oil palm seeds are intermediate according to Ellis et al (1991). Camillo et al (2009a) developed a protocol for seed cryopreservation of five E. guineensis genotypes in which seeds were immersed in LN, stored for 7 d, and slow thawed at room temperature (25±3°C) for 12 h. The seed tegument was removed following LN storage; the zygotic embryos were excised and inoculated on half-strength MS medium supplemented with 2 mg/L pantothenic acid, 3% (w/v) sucrose, and 0.25% (w/v) Phytagel. After 28 d, in vitro zygotic embryo germination rates varied from 73% to 100%, depending on the genotype.…”

Section: Cryopreservation Of Economically Important Non-native and Namentioning

confidence: 99%

In vitro and cryogenic preservation of plant biodiversity in Brazil

Pilatti

Aguiar

Simões

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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The Brazilian biomes (Amazon, Atlantic Forest, Cerrado, Caatinga, Pantanal, and Pampa) comprise one of the highest levels of plant diversity in the world; however, non-sustainable practices, deforestation, and land use have resulted in significant losses and fragmentation of the native forests. These ecosystems are now threatened and protection of their native plants through ex situ conservation is an urgent necessity. Cryopreservation and in vitro conservation are complementary options for securing and protecting Brazil's native plant species because their potential economic value is critically important to develop strategies that will (1) support their sustainable utilization, (2) protect against the over-exploitation of species growing in natural habitats, and (3) conserve the genetic diversity of germplasm from species of different provenances. Biotechnological approaches will help to address future economic and environmental demands placed upon already at-risk species. Conserving seed germplasm ex situ provides an additional safeguard against the risks (e.g., loss due to disease, climate change) of field conservation. Moreover, seed banks and cryobanks permit the long-term conservation of a wider genetic base; this offsets the labor and space intensive costs of conserving in the active growing state. This paper is a compilation of the current status of strategies applied for conserving Brazilian native plant species.

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Tolerância de sementes de dendezeiro à criopreservação

Cited by 13 publications

References 4 publications

Potencial germinativo e morfoanatomia foliar de plântulas de pinhão-manso originadas de germoplasma criopreservado

Potencial germinativo e morfoanatomia foliar de plântulas de pinhão-manso originadas de germoplasma criopreservado

A method for seedling recovery in <i>Jatropha curcas</i> after cryogenic exposure of the seeds.

In vitro and cryogenic preservation of plant biodiversity in Brazil

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