Estreptomicetos no controle da meloidoginose em mudas de tomateiro

Sousa, Carmen; Soares, Ana Cristina Fermino; Garrido, Marlon da Silva; Almeida, Gabriela Maria Carneiro de Oliveira

doi:10.1590/s0100-204x2006001200010

Cited by 13 publications

(10 citation statements)

References 17 publications

(11 reference statements)

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“…They came from the culture collection of the Phytopathology and Microbiology Laboratory of the Agricultural, Environmental and Biological Sciences Center, at Federal University of Recôncavo of Bahia (UFRB) State of Bahia, Brazil. These streptomycetes were isolated from rhizosphere soil of several crops by Lima (2002), and have been selected for plant growth promotion, control of tomato bacteria wilt (Lima, 2002), plant parasitic nematodes Sousa et al, 2005;Sousa et al, 2006) and phytopathogenic fungi .…”

Section: Methodsmentioning

confidence: 99%

Characterization of streptomycetes with potential to promote plant growth and biocontrol

Sousa¹,

Soares

Garrido³

2008

Sci. agric. (Piracicaba, Braz.)

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Studies with streptomycetes in biocontrol programs and plant growth promotion are presented as technological alternatives for environmental sustainable production. This work has the objective of characterizing six isolates of streptomycetes aiming the production of extracellular enzymes, indole acetic acid, capacity for phosphate solubilization, root colonization and growth under different pH and salinity levels. For detection of enzyme activity the isolates were grown in culture media with the enzyme substrates as sole carbon source. The root colonization assay was performed on tomato seedlings grown on 0.6% water-agar medium. Growth under different pH and salinity levels was evaluated in AGS medium with 1%, 1.5%, 2%, 2.5%, and 3% NaCl, and pH levels adjusted to 5.0, 5.5, 6.0, 6.5, and 7.0. All isolates produced the enzymes amylase, catalase, and lipase, as well as indole acetic acid. With one exception (AC-92), all isolates presented cellulolytic and chitinolytic activity, and only AC-26 did not show xylanolytic activity. The isolates AC-147, AC-95, and AC-29 were the highest producers of siderophores. The isolates AC-26 and AC-29 did not show capacity for phosphate solubilization. All isolates colonized tomato roots in vitro, and AC-92 grew under all pH and salinity levels tested. The streptomycetes tested were considered as potential biocontrol and plant growth promotion agents. Key words: actinomycetes, indole acetic acid, extracellular enzymes, siderophores CARACTERIZAÇÃO DE ESTREPTOMICETOS COM POTENCIAL PARA PROMOÇÃO DE CRESCIMENTO DE PLANTAS E BIOCONTROLERESUMO: Estudos com estreptomicetos em programas de biocontrole e promoção de crescimento de plantas são alternativas tecnológicas de produção sustentáveis ao ambiente. O presente trabalho teve como objetivo caracterizar seis isolados de estreptomicetos quanto à produção de enzimas extracelulares, ácido indolacético e sideróforos, capacidade de solubilização de fosfatos, colonização radicular e de crescimento em diferentes níveis de pH e salinidade. Para detectar a produção das enzimas extracelulares, os isolados foram cultivados em meio de cultura contendo os substratos dessas enzimas, como única fonte de carbono. Para determinar a capacidade de colonizar o sistema radicular, sementes de tomate foram cultivadas em meio ágar-água 0,6% e inoculadas com os estreptomicetos. O crescimento em diferentes níveis de salinidade e pH foram avaliados em meio de cultura AGS, com os níveis de NaCl 1%, 1,5%, 2%, 2,5% e 3% e níveis de pH ajustados em 5,0, 5,5, 6,0, 6,5 e 7,0.

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Section: Methodsmentioning

confidence: 99%

Characterization of streptomycetes with potential to promote plant growth and biocontrol

Sousa¹,

Soares

Garrido³

2008

Sci. agric. (Piracicaba, Braz.)

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“…Algumas espécies microbianas, como os estreptomicetos e Bacillus subtilis, foram avaliadas como interferentes no ciclo dos fitonematóides apresentando potencial para utilização no controle dos parasitas no solo (Kavitha et al 2007;Sousa et al, 2006). Ficou demonstrado que isolados de B. subtilis não apenas produzem metabólitos tóxicos que afetam o movimento de nematóides mas também podem inibir a eclosão de juvenis e o processo pelo qual eles penetram nas raízes (Araújo et al, 2002).…”

Section: Introductionunclassified

Multiplicação de Bacillus subtilis em vinhaça e viabilidade no controle da meloidoginose, em cana-de-açúcar

Cardozo

Araújo

2011

Rev. bras. eng. agríc. ambient.

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Objetivou-se, com este trabalho, avaliar o controle da meloidoginose e o crescimento da cana-de-açúcar, em função da aplicação de Bacillus subtilis ao solo após multiplicação em vinhaça. Bioensaios foram conduzidos em laboratório com avaliação do crescimento de B. subtilis em meio líquido com interesse na melhor composição de meio de cultura, a partir da vinhaça. No experimento em casa de vegetação foi utilizado solo coletado em área de cultivo de cana com histórico de alta infestação com Meloidogyne spp. No solo acondicionado em vasos efetuou-se o plantio da variedade de cana-de-açúcar RB 72454, cujos tratamentos foram: controle; vinhaça pura (50 m³ ha-1); Bacillus subtilis em suspensão aquosa (50 m³ ha-1); B. subtilis multiplicado na vinhaça para 50 m³ ha-1 e 100 m³ ha-1. A multiplicação de Bacillus subtilis em meio de cultura à base de vinhaça (25%) foi significativamente superior em comparação com o meio de cultura caldo nutriente. A aplicação de B. subtilis em suspensão aquosa promoveu o crescimento e a redução da reprodução dos nematóides em cana-de-açúcar durante o experimento. A aplicação de B. subtilis multiplicado na vinhaça não proporcionou os benefícios de estímulo ao crescimento e controle da meloidoginose na cana-de-açúcar, encontrados com a aplicação apenas da bactéria no solo.

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“…N0035, S. purpurascens and S. griseus subspecies griseus rice inoculum caused significant reduction in the number of root galls and egg masses in tomato plants inoculated with M. incognita. Growth promotion was also observed in tomato plants grown in pots with planting mix inoculated with Streptomyces rice inoculum (Sousa, 2006;Sousa et al, 2006). In addition, these Streptomyces species have been indicated as potential biological agents for controlling yam (Dioscorea cayennensis Lam.)…”

Section: Resultsmentioning

confidence: 99%

“…griseus, Streptomyces sp. N0035, and S. purpurascens, which had been selected in previous work as potential biocontrol agents (Lima, 2003;Soares et al, 2006;Sousa et al, 2006). The cell suspension of each Streptomyces isolate was prepared by adding 15 mL of sterile distilled water to each Petri dish and the Streptomyces colonies were scraped with a bacterial loop.…”

Section: Methodsmentioning

confidence: 99%

“…These microorganisms have an important role in the degradation of polymers such as lignin, chitin, cellulose, and starch (Crawford, 1988;Nolan & Cross, 1988), the control of plant pathogens (Getha et al, 2005;Minuto et al, 2006;Sousa et al, 2006), as well as in the production of other important secondary metabolites of industrial interest (Lancini & Lorenzetti, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Production of streptomycete inoculum in sterilized rice

Soares¹,

Sousa

Garrido

et al. 2007

Sci. agric. (Piracicaba, Braz.)

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Actinomycetes are important plant disease control and growth promotion agents, which makes it necessary to develop technology to produce large quantities of inoculum for green-house and field work. The present study had the objective of evaluating the growth of several isolates of Streptomyces in sterile rice for inoculum production. The sterile rice was inoculated with isolates of S. thermotolerans, S. griseus subsp. griseus, Streptomyces sp. N0035, S. purpurascens, and Streptomyces sp., and incubated at 28 ± 2ºC. Five days after its inoculation, mycelial growth and sporulation was observed for all Streptomyces isolates on the rice grains. Twelve days after incubation, the colonized rice was transferred to envelopes of dark brown paper and let to dry in an incubator at 30ºC for three days. After drying, 1g of colonized rice was added to 200 mL of sterile distilled water and the number of spores was counted under a microscope with a Newbauer counting chamber. Spore production varied from 0.14 × 10 9 to 1.47 × 10 9 spores per gram of rice and differed among the Streptomyces species. Sterile rice can be an alternative substrate for low cost mass production of Streptomyces inoculum.

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Estreptomicetos no controle da meloidoginose em mudas de tomateiro

Cited by 13 publications

References 17 publications

Characterization of streptomycetes with potential to promote plant growth and biocontrol

Characterization of streptomycetes with potential to promote plant growth and biocontrol

Multiplicação de Bacillus subtilis em vinhaça e viabilidade no controle da meloidoginose, em cana-de-açúcar

Production of streptomycete inoculum in sterilized rice

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