Detrimental effect of rutin on Anticarsia gemmatalis

Abstract

-Behavioral and nutritional effect of rutin (quercetin 3-O-rutinosídeo) on Anticarsia gemmatalis Hübner (Lep.: Noctuidae), a major soybean defoliator in Brazil, was evaluated from the third instar to pupation. Rutin is one of the flavonol glycosides identified in the leaves of the wild soybean PI 227687. Larval weight and amount of ingested food decreased as rutin concentration in the diet increase. An interactive effect between feeding time and diet (treatment) was observed on insect growth; when larvae fed on pure-diet, feeding time elongation resulted in heavier pupae. Differently, the weight of larvae fed on rutin-diet remained almost stable, in spite of eating for longer. A. gemmatalis growth was negatively influenced by rutin-diet not only by feeding deterrence but also by post-ingestive effect on insect growth, since after adjustment of pupal weight by the amount of ingested food (covariate), the effect of diet remained significant. Rutin negatively influenced A. gemmatalis growth as result of pre-ingestive effect, indicated by reduction in food consumption, and post-ingestive effect, indicated by lower conversion of ingested food into body mass and food assimilation.Index terms: Glycine max, feeding deterrence, insect nutrition, flavonol glycoside, Lepidoptera. Efeito prejudicial de rutina em Anticarsia gemmatalisResumo -O efeito de dieta contendo rutina (quercetina 3-O-rutinosídeo) no comportamento e na nutrição do principal desfolhador da soja, Anticarsia gemmatalis Hübner (Lep.: Noctuidae) foi avaliado do terceiro ínstar até a formação da pupa. Rutina é um dos flavonóis glicosídicos identificados em folhas da soja selvagem PI 227687. O peso das lagartas e o consumo decresceram com o aumento na concentração de rutina na dieta. Houve interação entre o tempo de alimentação e a dieta no crescimento do inseto; lagartas alimentadas com dieta pura, cujo tempo de alimentação prolongou-se, originaram pupas mais pesadas. O peso das pupas alimentadas com dieta contendo rutina permaneceu estável, apesar do maior tempo de alimentação. Rutina afetou negativamente o crescimento do inseto, não somente pela inibição alimentar, mas também em conseqüência de eventos pós-ingestivos, já que depois do ajuste do peso de pupa pelo consumo (covariável), o efeito do tratamento permaneceu significativo. Assim, o efeito negativo de rutina em A. gemmatalis resulta de efeitos pré-ingestivos, indicados pela redução no consumo, e pós-ingestivos, indicados por baixas conversões do alimento ingerido em biomassa e assimilação de alimentos.Termos para indexação: Glycine max, inibição alimentar, nutrição de insetos, flavonol glicosídico, Lepidoptera.

Bioactivity of ethanolic extracts of Euphorbia pulcherrima on Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

“…According to Torres et al (2001) this extension may be related to the presence of growth inhibitors, low conversion of ingested food or by contain toxic substances that interfere with the lower food intake. This result is corroborated by Piubelli (2004) and Hoffmann-Campo et al (2006) who have studied biological aspects of Anticarsia gemmatalis (Lepidoptera: Noctuidae), fed with diets with the addition of the rutin flavonoid, observed an extension in feeding time.…”

<b>Effect of the flavonoid rutin on the biology of <i>Spodoptera frugiperda</i> (Lepidoptera: Noctuidae)

“…Spodoptera frugiperda control has been mainly performed with chemical insecticides (Yu, Nguyen, & Abo-Elghar, 2003 and the use of plant metabolites with insecticidal effects have shown promising results in integrated pest management programs (Pereira et al, 2002;Hoffmann-Campo, Ramos Neto, Oliveira, & Oliveira, 2006;Jesus et al, 2014). Resistance can be express by morphological characteristics or the presence of chemical metabolites (allelochemicals), such as alkaloids, flavonoids, terpenoids, sterols etc., present in the plants (Piubelli, Campo, Moscardi, Miyakubo, & Oliveira, 2005;Hoffmann-Campo et al, 2006;Magarelli, Lima, Silva, Souza, & Castro, 2014).…”

“…Resistance can be express by morphological characteristics or the presence of chemical metabolites (allelochemicals), such as alkaloids, flavonoids, terpenoids, sterols etc., present in the plants (Piubelli, Campo, Moscardi, Miyakubo, & Oliveira, 2005;Hoffmann-Campo et al, 2006;Magarelli, Lima, Silva, Souza, & Castro, 2014).…”

“…In soybean, the flavonoids rutin and genistin were identified in several parts of the plant (Piubelli et al, 2005;Hoffmann-Campo et al, 2006;Lucci & Mazzafera, 2009).…”

“…Rutin can prolong the life cycle of Anticarsia gemmatalis Hubner, 1818 (Lepidoptera: Noctuidae) and cause higher larval mortality when it is added to the insect diet (Gazzoni, Hulsmeyer, & HoffmannCampo, 1997;Hoffmann-Campo et al, 2006;Piubelli, Hoffmann-Campo, Moscardi, Miyakubo, & Oliveira, 2006). Larvae of Manduca sexta Linnaeus, 1763 (Lepidoptera: Sphingidae) fed a diet containing rutin showed negative growth performance (Stamp & Skrobola, 1993).…”