Isolamento de Gluconacetobacter spp. em diferentes tipos de solos

Abstract

O objetivo deste trabalho foi o aprimoramento da técnica de imunocaptura para utilização em amostras de solo contendo altos teores de argila e sua aplicação no isolamento de estirpes de Gluconacetobacter diazotrophicus a partir de amostras de solo cultivado com cana-de-açúcar e café. A técnica de imunocaptura foi aplicada com sucesso no isolamento de bactérias de amostras de solo. A modificação do método de imunocaptura com Al2(SO4)3 permitiu a sua aplicação em amostras de solo argiloso pela floculação da argila em suspensão. Este método mostrou-se efetivo no isolamento de G. diazotrophicus inoculada em amostras de solo arenoso e argiloso aos cinco dias após a inoculação. A sensibilidade máxima do método em isolar células de G. diazotrophicus mediante cultura pura foi de 10³ células mL-1 . A modificação da técnica permitiu o isolamento de Gluconacetobacter spp. de amostras de solo colhidas a 50 cm das raízes de plantas de café, mas não de amostras de solo colhidas à mesma distância de plantas de cana-de-açúcar.

The Traits of the Plant Growth Promoting Acetic Acid Bacterium, Nguyenibacter Vanlangensis

“…Acetobacter diazotrophicus that was later renamed Gluconacetobacter diazotrophicus was considered as the first PGPB representative of the family Acetobacteraceae to be reported with the capacity of nitrogen fixation [7][8][9]. There are lots of reports on the plant growth promoting mechanisms of G. diazotrophicus as well as its different kinds of isolation sources [5,[10][11][12][13]. After the introduction of novel nitrogen-fixing acetic acid bacteria associated with coffee plants of the two species, G. johannae and G. azotocaptans, G. azotocaptans strain DS1 was reported to show additional plant growth promoting mechanisms such as producing IAA (indole-3-acetic acid, heteroauxin), solubilizing phosphate, and providing biological control against several fungal pathogens and to promote the growth of some corn varieties [14][15][16].…”

Colonization and yield promotion of tomato by Gluconacetobacter diazotrophicus

“…Gluconacetobacter diazotrophicus is a N 2 -fixing bacterium originally associated with sugarcane as described by Cavalcante and Döbereiner (1988), but it has also been found in natural endophytic association with other host plants such as sweet potato (Paula et al, 1991), cameron grass (Döbereiner et al, 1993), coffee (Jiménez-Salgado et al, 1997), finger millet (Loganathan et al, 1999) and pineapple (Tapia-Hernández et al, 2000). Its natural occurrence in the rhizosphere of different plants has also been documented (Jiménez-Salgado et al, 1997;Loganathan et al, 1999;Muthukumarasamy et al, 2005;Santos dos et al, 2006). The interaction of G. diazotrophicus with sugarcane represents a model system for monocot-diazotrophic association and even though this relation is not yet fully understood, different reports indicate that G. diazotrophicus seems to be able to promote plant growth (for a review see Pedraza, 2008).…”