Screening of Bacillus thuringiensis strains effective against mosquitoes

Monnerat, R. G.; Dias, Daniel Gerhein Souza; Silva, Silvania Ferreira da; Martins, E. S.; Berry, Colin; Falcão, Rosana; Gomes, Ana C.; Praça, L. B.; Soares, C. M.

doi:10.1590/s0100-204x2005000200001

Cited by 11 publications

(5 citation statements)

References 6 publications

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“…The isolates produced 1-5 diVerent protein bands and about 70% of the isolates showed more than one band. Based on previous studies [20,27,32], major Dipteran-active Cry and Cyt toxins have molecular masses of 17-28 (Cry17, Cyt), 50 (Cry2Ab), 65-75 (Cry2Aa, Cry2Ac, Cry4D, Cry17A, Cry19, Cry24), 80 (Cry4C, Cry11B) and 128-140 kDa (Cry4A, Cry4B, Cry32). Thus, based on these studies, band patterns were divided in to six diVerent speciWc patterns (Fig.…”

Section: Resultsmentioning

confidence: 96%

Distribution and diversity of Dipteran-specific cry and cyt genes in native Bacillus thuringiensis strains obtained from different ecosystems of Iran

Jouzani

Abad

Seifinejad

et al. 2007

J Ind Microbiol Biotechnol

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One hundred and twenty-eight Bacillus thuringiensis isolates from fields of different ecological regions of Iran were collected to study the distribution and diversity of Dipteran-specific cry and cyt genes. The percentage of samples with Bt showed significant differences between different regions and also between different fields. The most Bt frequency was observed in the soil samples collected from Caspianic zone (7%) and soils of cotton (17%). Characterization of isolates was based on morphological characteristics of crystals, plasmid profiles and protein band patterns as well as PCR analysis using general and specific primers for 22 different cry and cyt genes encoding proteins active against mosquitoes. Thirty-eight different cry gene profiles were detected in this collection. Several of them were found to be different from all previously published profiles and none of the previous researches reported these numbers of profiles. Strains containing cry2-type genes were the most abundant and represent 57.1% of the isolates. Strains harboring cry24 and cry10 genes were also highly abundant (38.7 and 32.8%, respectively). cry11, cry4, cry17, cry19, cry21, cry29, cyt1, and cry9 genes were less abundant, found in 25.7, 14.3, 11.4, 1.4, 4.3, 1.4, and 10% of the strains, respectively. Among the cry2 gene containing isolates, 37.5% strains harbored cry2Aa, 55% cry2Ab, 2.5% cry2Ac, and 5% other or novel cry2-type genes. Among the cry4 gene containing isolates, 0% strains harbored cry4A, 60% cry4B, and 40% cry4C, cry4D or novel cry4 type genes. Finally, based on crystal morphology, protein patterns and PCR, 21 strains were selected as potentially high Dipteran-active for bioassays. Also our results showed that some of the isolates may harbor minimum a putative novel cry gene.

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Section: Resultsmentioning

confidence: 96%

Distribution and diversity of Dipteran-specific cry and cyt genes in native Bacillus thuringiensis strains obtained from different ecosystems of Iran

Jouzani

Abad

Seifinejad

et al. 2007

J Ind Microbiol Biotechnol

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“…, 1990 ; Monnerat et al. , 1999 ), Culex quinquefasciatus (Diptera: Culicidae), Aedes aegypti (Diptera: Culicidae) ( Monnerat et al. , 2005 ) and Anthonomus grandis (Coleoptera: Curculionidae) ( Martins et al.…”

Section: Methodsmentioning

confidence: 99%

Translocation and insecticidal activity of Bacillus thuringiensis living inside of plants

Monnerat

Soares²,

Capdeville

et al. 2009

Microbial Biotechnology

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SummaryThe major biological pesticide for the control of insect infestations of crops, Bacillus thuringiensis was found to be present naturally within cotton plants from fields that had never been treated with commercial formulations of this bacterium. The ability of B. thuringiensis to colonize plants as an endophyte was further established by the introduction of a strain marked by production of green fluorescent protein (GFP). After inoculation of this preparation close to the roots of cotton and cabbage seedlings, GFP‐marked bacteria could be re‐isolated from all parts of the plant, having entered the roots and migrated through the xylem. Leaves taken from the treated plants were able to cause toxicity when fed to the Lepidoptera Spodoptera frugiperda (cotton) and Plutella xylostella (cabbage). These results open up new horizons for understanding the natural ecology and evolution of B. thuringiensis and use of B. thuringiensis in insect control.

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“…Two were Bacillus thuringiensis strains of different serotypes: B. thuringiensis serotype kurstaki (BTK), encoding Cry1Aa, Cry1Ab, Cry1Ac and Cry1B proteins, toxic to lepidopteran larvae ) and B. thuringiensis serotype israelensis (BTI), encoding Cry4A, Cry4B, Cry11 and cyt1 proteins, toxic to dipteran larvae (Monnerat et al 2005). Two more were strains of B. sphaericus serotype H5 (BS1 and BS2) encoding 51 and 42 kDa proteins, toxic to dipteran larvae ).…”

Section: Entomopathogens Testedmentioning

confidence: 99%

Susceptibility of non-target invertebrates to Brazilian microbial pest control agents

Oliveira-Filho

Muniz²,

Freire

et al. 2011

Ecotoxicology

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Microbial pest control agents or entomopathogens have been considered an interesting alternative to use instead of chemical insecticides. Knowledge of ecotoxicity data is very important to predict the hazard of any product released in the environment and subsidize the regulation of these products by governmental agencies. In the present study four new Brazilian strains of Bacillus and one fungus were tested to evaluate their acute toxicity to the microcrustacean Daphnia similis, the snail Biomphalaria glabrata and the dung beetle Digitonthophagus gazella. The microcrustaceans and the snails were exposed to entomopathogens in synthetic softwater and the beetles were exposed directly in cattle dung. Obtained data reveal low susceptibility of the non-target species to tested microorganisms, with lethal concentrations being observed only at much higher concentrations than that effective against target insects. These results show that the tested strains are selective in their action mode and seem to be non-hazardous to non-target species.

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Screening of Bacillus thuringiensis strains effective against mosquitoes

Cited by 11 publications

References 6 publications

Distribution and diversity of Dipteran-specific cry and cyt genes in native Bacillus thuringiensis strains obtained from different ecosystems of Iran

Distribution and diversity of Dipteran-specific cry and cyt genes in native Bacillus thuringiensis strains obtained from different ecosystems of Iran

Translocation and insecticidal activity of Bacillus thuringiensis living inside of plants

Susceptibility of non-target invertebrates to Brazilian microbial pest control agents

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