Caracterização bioquímica e cinética de lipoxigenases de plantas de soja submetidas à aplicação de ácidos graxos poliinsaturados

Batista, Rosa Bárbara; Oliveira, Maria Goreti de Almeida; Pires, Christiano Vieira; Piovesan, Newton Deniz; Rezende, Sebastião Tavares de; Moreira, Maurílio Alves

doi:10.1590/s0100-204x2002001100001

Cited by 8 publications

(8 citation statements)

References 20 publications

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“…The powder obtained was homogenized in a 50 mM sodium phosphate buffer, pH 6.5 at a ratio of 1:3 (w/v) and centrifuged at 17,200 g for 60 min at 4 ºC, following modifications of the methodology of Batista et al (2002). The total protein concentration, LOX activity and the concentration of PI were determined in the supernatant, named the 'crude extract'.…”

Section: Leaf Extractmentioning

confidence: 99%

Active response of soybean to defoliator Anticarsia gemmatalis Hübner: strategies to overcome protease inhibitor production

Paixao

Lourenção

Silva

et al. 2016

Idesia

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Endogenous mechanisms of plant resistance to herbivorous insects could be adapted to enable the development of alternative strategies for pest control. Activation of the lipoxygenase (LOX) pathway by jasmonic acid, the hormone responsible for activating genes encoding protease inhibitors (PI), is one of the main direct defenses of plants against insects. In this study, soybean cultivars at the V3 stage that were either susceptible or resistant to herbivorous insects were used to investigate changes in the activity of LOX and concentration of PIs in response to 24-or 48-h feeding by fourth and fifth instar Anticarsia gemmatalis (Lepidoptera: Noctuidae) larvae. LOX activity (0.0-0.007 nm s -1 /mg) and the PI concentration (60 to 125 mg of trypsin inhibitor / mg of protein and 90 to 120 mg of trypsin inhibitor / mg protein after 24 and 48 h after attack, respectively) both increased in response to damage caused by A. gemmatalis. Proteolytic activity decreased by approximately 50% in cultivars with different degrees of resistance. Raised enzyme profiles in the midgut of A. gemmatalis may be related to tryptic enzymes that increased, compensating for the inhibition of proteases by entomotoxic substances of the soybean cultivars.Key words: plant defense, lipoxygenases, velvet caterpillar. RESUMEN

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Section: Leaf Extractmentioning

confidence: 99%

Active response of soybean to defoliator Anticarsia gemmatalis Hübner: strategies to overcome protease inhibitor production

Paixao

Lourenção

Silva

et al. 2016

Idesia

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“…Nesse sentido, acredita-se que esses frutos apresentariam maior eficiência no controle microbiológico, visto que as lipoxigenases estão presentes em inúmeros processos fisiológicos do fruto, principalmente Figura 2 -Atividade da Lipoxigenase (nkat g -1 peso fresco) em tomates 'Pitenza' irradiados com UV-C e armazenados a 12 ± 1 ºC e 90-95% UR, por 21 dias. quanto aos mecanismos de defesa (BATISTA et al, 2002). Dados esses concordantes com González-Aguilar et al (2007), que também não verificaram mudanças significativas na atividade da lipoxigenase em mangas ao longo do armazenamento, utilizando-se doses de radiação entre 2,46 e 4,93 kJ m -2 .…”

Section: Atividade Da Lipoxigenaseunclassified

Qualidade do tomate ‘PITENZA’ com utilização da radiação ultravioleta (UV-C)

et al. 2015

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mesmo quando submetido à refrigeração. Assim, objetivou-se com este trabalho avaliar o efeito de doses de radiação ultravioleta (UV-C) em diferentes tempos de conservação de tomates 'Pitenza' refrigerados. Os frutos foram colhidos e imediatamente transportados ao laboratório, onde foram mantidos a 12 ± 1 0 C, por 12 horas. Posteriormente, os frutos foram padronizados, sendo submetidos às doses de radiação UV-C 0 (controle); 2,27; 4,54 e 7,19 kJ m -2 e armazenados sob refrigeração a 12 ± 1 o C e 90-95% de UR, por 21 dias. Realizaram-se análises a cada 7 dias, em um período de 21 dias, quanto ao potencial hidrogeniônico, ácido ascórbico, peroxidase, licopeno e lipoxigenase. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 4x4 (dose x tempo), com 3 repetições. Na atividade enzimática, bem como nos teores de licopeno e ácido ascórbico, não foi verificada a ocorrência da interação entre os fatores dose x tempo, somente sendo evidenciada a diferença estatística nas doses de radiação. Ao final, observou-se que os frutos submetidos às doses de 2,27 e 4,54 kJ m -2 demonstraram efeito positivo da radiação UV-C sobre a qualidade dos tomates. Palavras-chave:Radiação, Solanum lycopersicum, Pós-colheita. Abstract:The tomato easily deteriorates post-harvest, even under refrigeration. The aim of this work therefore was to evaluate the effect of levels of ultraviolet radiation (UVC) for different periods of storage, on refrigerated 'Pitenza' tomatoes. The fruit were harvested and immediately transported to the laboratory where they were kept at 12 ± 1 °C for 12 hours. The fruit were sorted, and later subjected to levels of UVC radiation: 0 (control), 2.27, 4.54 and 7.19 kJ m -2. They were then stored under refrigeration at 12 ± 1 °C and 90-95% RH for 21 days. Every 7 days for a period of 21 days, an analysis was made of the hydrogen ion potential, ascorbic acid, peroxidase, lycopene and lipoxygenase. The experimental design was completely randomised, in a 4x4 factorial (level x time), with three replications. For enzyme activity, as well as for lycopene and ascorbic acid levels, no interaction was seen between the factors level x time, with a statistical difference only being noted for the level of radiation. By the end, it could be seen that fruits submitted to levels of 2.27 and 4.54 kJ m -2 displayed a positive effect on the quality of the tomatoes from the UVC radiation.

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“…The mixture obtained was homogenized using a Vortex mixer model Brazil). This mixture was centrifuged in a refrigerated microcentrifuge (model CT 15000R) to at 17,200 g for 30 minutes (Batista et al 2002). The supernatant of the mixture was collected using a pipette (Gilson) and stored in a 2-ml Eppendorf.…”

Section: O Ilicis Colonymentioning

confidence: 99%

Trypsin protease inhibitor activity is not a good proxy for defence againstOligonychus ilicis(Acari: Tetranychidae) inCoffea canephora(Gentianales: Rubiaceae)

Silva

Ribeiro

Queiroz

et al. 2015

International Journal of Acarology

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Oligonychus ilicis is one of the most important pests that causes injury to the Robusta Coffee leaves, but no data is available on the production of protease inhibitors (PIs) in Coffea. canephora plants, either sensitive or resistant to O. ilicis. This work aimed to determine the C. canephora genotypes susceptible and resistant to O. ilicis and the activity of PIs in these genotypes. In the first trial, we assessed the susceptibility of 14 C. canephora genotypes to O. ilicis and found considerable variation. The Significant differences in the fecundity, net reproductive rate and intrinsic rate of population increase were observed among different genotypes of C. canephora. In the second one, we assessed the activity of PIs in leaves of the 14 C. canephora genotypes damaged and non-damaged by O. ilicis. The contents of the PI were influenced by genotypes, presence of mites and the interaction of the both factors. In the susceptible genotypes, an increase in the activity of trypsinlike PIs is predominant, while in the resistant genotypes a reduction in this activity predominates. These findings indicate that trypsin PI activity is not a good proxy for defence against O. ilicis.

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Caracterização bioquímica e cinética de lipoxigenases de plantas de soja submetidas à aplicação de ácidos graxos poliinsaturados

Cited by 8 publications

References 20 publications

Active response of soybean to defoliator Anticarsia gemmatalis Hübner: strategies to overcome protease inhibitor production

Active response of soybean to defoliator Anticarsia gemmatalis Hübner: strategies to overcome protease inhibitor production

Qualidade do tomate ‘PITENZA’ com utilização da radiação ultravioleta (UV-C)

Trypsin protease inhibitor activity is not a good proxy for defence againstOligonychus ilicis(Acari: Tetranychidae) inCoffea canephora(Gentianales: Rubiaceae)

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