Sucrose metabolizing enzymes in cell suspension cultures of Bauhinia forficata, Curcuma zedoaria and Phaseolus vulgaris

Mello, Marcia O.; Amaral, Antônio Francisco de Campos; Melo, Murilo

doi:10.1590/s0100-204x2001000900001

Cited by 4 publications

(3 citation statements)

References 27 publications

(22 reference statements)

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“…The same was also noted in our previous study where the same molecule was shown to be induced by activators of plant defence and priming responses [8]. The differences in the metabolite profiles (including CGAs and phenolic content) between cells and leaf tissue have been previously reported [21,29]. However, according to the best of our knowledge, this is the first report focusing on the differences between cells and leaf tissue samples taking into account both regional and geometrical isomerism ( cis and trans configurations) of CGAs.…”

Section: Resultssupporting

confidence: 81%

Analyses of chlorogenic acids and related cinnamic acid derivatives from Nicotiana tabacumtissues with the aid of UPLC-QTOF-MS/MS based on the in-source collision-induced dissociation method

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Mhlongo

Piater

et al. 2014

Chemistry Central Journal

128

104

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BackgroundChlorogenic acids (CGAs) are a class of phytochemicals that are formed as esters between different derivatives of cinnamic acid and quinic acid molecules. In plants, accumulation of these compounds has been linked to several physiological responses against various stress factors; however, biochemical synthesis differs from one plant to another. Although structurally simple, the analysis of CGA molecules with modern analytical platforms poses an analytical challenge. The objective of the study was to perform a comparison of the CGA profiles and related derivatives from differentiated tobacco leaf tissues and undifferentiated cell suspension cultures.ResultsUsing an UHPLC-Q-TOF-MS/MS fingerprinting method based on the in-source collision induced dissociation (ISCID) approach, a total of 19 different metabolites with a cinnamic acid core moiety were identified. These metabolites were either present in both leaf tissue and cell suspension samples or in only one of the two plant systems. Profile differences point to underlying biochemical similarities or differences thereof.ConclusionUsing this method, the regio- and geometric-isomer profiles of chlorogenic acids of the two tissue types of Nicotiana tabacum were achieved. The method was also shown to be applicable for the detection of other related molecules containing a cinnamic acid core.

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Section: Resultssupporting

confidence: 81%

Analyses of chlorogenic acids and related cinnamic acid derivatives from Nicotiana tabacumtissues with the aid of UPLC-QTOF-MS/MS based on the in-source collision-induced dissociation method

Ncube

Mhlongo

Piater

et al. 2014

Chemistry Central Journal

128

104

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“…The impact of exposure to thermal stresses could be a trigger for the cell to opt for sucrose breakdown via the classical pathway. The regulation of sucrose between the two pathways varies according to the availability of sucrose, growth stage, tissue function and the plant species . Invertases have been proposed to function in response to cold and to channel sucrose into catabolism in contrast to sucrose synthase which channels sucrose into anabolism .…”

Section: Resultsmentioning

confidence: 99%

Quantitative proteomic analysis of cabernet sauvignon grape cells exposed to thermal stresses reveals alterations in sugar and phenylpropanoid metabolism

et al. 2015

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Grapes (Vitis vinifera) are a valuable fruit crop and wine production is a major industry. Global warming and expanded range of cultivation will expose grapes to more temperature stresses in future. Our study investigated protein level responses to abiotic stresses, with particular reference to proteomic changes induced by the impact of four different temperature stress regimes, including both hot and cold temperatures, on cultured grape cells. Cabernet Sauvignon cell suspension cultures grown at 26°C were subjected to 14 h of exposure to 34 and 42°C for heat stress, and 18 and 10°C for cold stress. Cells from the five temperatures were harvested in biological triplicates and label-free quantitative shotgun proteomic analysis was performed. A total of 2042 non-redundant proteins were identified from the five temperature points. Fifty-five proteins were only detected in extreme heat stress conditions (42°C) and 53 proteins were only detected at extreme cold stress conditions (10°C). Gene Ontology (GO) annotations of differentially expressed proteins provided insights into the metabolic pathways that are involved in temperature stress in grape cells. Sugar metabolism displayed switching between alternative and classical pathways during temperature stresses. Additionally, nine proteins involved in the phenylpropanoid pathway were greatly increased in abundance at extreme cold stress, and were thus found to be cold-responsive proteins. All MS data have been deposited in the ProteomeXchange with identifier PXD000977 (http://proteomecentral.proteomexchange.org/dataset/PXD000977).

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“…Since Nickell (1964) de vel oped the pro to col for sug ar cane cell sus pen sion cul tures, it has been used as a model sys tem to in ves ti gate sugar up take by the cells (Maretzki and Thom 1972, Komor et al 1981, Ebrahim et al 1999, trans port across tonoplast Maretzki 1985, Thom andKomor 1985) and su crose me tab o lism (Botha et al 1992, Mello et al 2001. The syn the sis of su crose oc curs via se quen tial ac tion of su crose phos phate synthase and su crose phosphatase.…”

Section: Introductionmentioning

confidence: 99%

Regulation of sucrose storage by amino acid arginine in sugarcane cell suspension cultures

2005

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Sug ar cane cell cul tures were ob tained from cal lus formed on explants de rived from young ex pand ing leaves of two early ma tur ing sug ar cane va ri et ies viz "CoJ83" and "CoJ86". The cell cul tures were var ied with dif fer ent arginine con cen tra tions in the cul ture me dium. For each cultivar, su crose con tent with 20 µM arginine in the cul ture me dium de creased from 3 to 5 days and then in creased to 10 days af ter subculturing. Higher con cen tra tion of arginine in the cul ture me dium (60 µM) decreased the su crose con tent at dif fer ent days af ter subculturing and thus sig nif i cantly stim u lated su crose mo bi li za tion. The activ ity of su crose synthase and su crose phos phate synthase reached max i mum while the ac tiv ity of acid and neu tral invertase was min i mal in the cul ture me dium with 20 µM arginine. Thus arginine at low con cen tra tion (20 µM) en ables the cells to ac cu mu late the higher level of su crose. The op timum level of amino ac ids can be uti lized to reg u late the in vivo ac tiv ity of su crose synthase, su crose phos phate synthase and invertase to achieve max i mum su crose ac cu mu la tion in sug arcane stor age tis sue.List of ab bre vi a tions: EDTA: Eth yl ene diamine tetra-ace tic acid; Hepes: N-(2-hydroxyethyl) pipera zine-N`-(2-ethanesulphonic acid); DETC: sodium di ethyl dithiocarbamate; DAS: days af ter sub culturing; MS: Murashige and Skoog In tro duc tionSug ar cane is a good ex am ple of a plant which stores large amount of su crose .The stor age of sucrose in sug ar cane internodes is a de vel op men tally reg u lated pro cess dur ing which the pa ren chyma cells af ter a pe riod of growth and ex pan sion start to ac cu mu late su crose (Lingle 1997, Batta et al. 2002. The young internodes use in com ing su crose for growth whereas the older internodes store sucrose (Glasziou and Gayler 1972) so that it accounts for 50 % of to tal dry weight, reach ing a concen tra tion of 500 mol⋅m -3 .Since Nickell (1964) de vel oped the pro to col for sug ar cane cell sus pen sion cul tures, it has been used as a model sys tem to in ves ti gate sugar up take by the cells (Maretzki and Thom 1972, Komor et al. 1981, Ebrahim et al. 1999, trans port across tonoplast Maretzki 1985, Thom and Komor 1985) and su crose me tab o lism (Botha et al. 1992, Mello et al. 2001. The syn the sis of su crose oc curs via se quen tial ac tion of su crose phos phate synthase and su crose phosphatase. The deg ra dation of su crose oc curs by sev eral en zymes in cluding su crose synthase and neu tral invertase in cytosol (Goldner et al.1991) and sol u ble acid invertase in vac u ole. The net su crose stor age is reg -37 ACTA PHYSIOLOGIAE PLANTARUM

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Sucrose metabolizing enzymes in cell suspension cultures of Bauhinia forficata, Curcuma zedoaria and Phaseolus vulgaris

Cited by 4 publications

References 27 publications

Analyses of chlorogenic acids and related cinnamic acid derivatives from Nicotiana tabacumtissues with the aid of UPLC-QTOF-MS/MS based on the in-source collision-induced dissociation method

Analyses of chlorogenic acids and related cinnamic acid derivatives from Nicotiana tabacumtissues with the aid of UPLC-QTOF-MS/MS based on the in-source collision-induced dissociation method

Quantitative proteomic analysis of cabernet sauvignon grape cells exposed to thermal stresses reveals alterations in sugar and phenylpropanoid metabolism

Regulation of sucrose storage by amino acid arginine in sugarcane cell suspension cultures

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