Intra-puparial development of the females of Chrysomya albiceps (Wiedemann) (Diptera, Calliphoridae)

Pujol-Luz, José Roberto; Barros-Cordeiro, Karine Brenda

doi:10.1590/s0085-56262012005000038

Cited by 29 publications

(20 citation statements)

References 19 publications

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“…This contrasts with the report of Pujol-Luz and Barros-Cordeiro (2012), where this same stage in C. albiceps lasted just 3 h when reared at 26 ºC, corresponding to 3% of the total development (Table 2).…”

Section: Discussioncontrasting

confidence: 89%

“…This data is in agreement with that observed by Pujol-Luz and Barros-Cordeiro (2012), who reported 81 h for C. albiceps. However, the starting point is different, being at 120 h for the current study, whereas it was at 9 h in the study by Pujol-Luz and Barros-Cordeiro (2012), which can probably be explained by the species belonging to different genera, beside the difference in relative humidity (RH) during their development, as the temperatures used in both studies were similar (25.46 ± 1.21 ºC, 93.31 ± 2.41% RH and 26 ± 1.0 ºC, 60 ± 10% RH, respectively).…”

Section: Amazonicasupporting

confidence: 94%

“…The sampled specimens were fixed in ethanol (96%), followed first by a treatment with Carnoy solution for 24 hours and then with formic acid (5%) for 48 hours. This chemical treatment, facilitated the removal of the puparium using the methodology proposed by Cepeda-Palacios and Scholl (2000), Pujol-Luz and Barros-Cordeiro (2012) and Barros-Cordeiro et al (2014).…”

Section: Methodsmentioning

confidence: 99%

“…All observations were documented and imaged using a photographic camera (Optika PRO 5 Digital Camera 4083.12, Italy) adapted to the stereoscope. In addition it is included the stage of pre-pupa, pupariation and the morphological description of the pupa and the beginning of the imago and emergence, according to Fraenkel and Bhaskaran (1973), Pujol-Luz and BarrosCordeiro (2012) and Barros-Cordeiro et al (2014) The methodology used in this study was adapted from Cepeda-Palacios and Scholl (2000) and Pujol-Luz and Barros-Cordeiro (2012). To describe the morphology of the intra-puparial stages we used the methodology of Fraenkel and Bhaskaran (1973), Cepeda-Palacios and Scholl (2000) and Pujol-Luz & Barros-Cordeiro (2012).…”

Section: Intra-puparial Developmentmentioning

confidence: 99%

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Intra-puparial development of Lucilia eximia (Diptera, Calliphoridae)

2017

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There are few studies about the intra-puparial development in Diptera, nonetheless its importance has been increased because several dipteran species are of forensic interest. Studies on the life cycles of flies often disregard the changes that occur inside the pupae. The objectives of this research were to analyze the intra-puparial development of Lucilia eximia, and to describe chronological and morphological changes that occur during this stage. Around 1.600 specimens were laboratory-reared. The pre-pupae were identified by the reduction of their size and change in coloration, and 10 individuals were sampled every three hours (n=1.000) until adult emergence. The specimens were fixed in 96% alcohol, subsequently immersed in Canoy solution for 24 hours and in formic acid (5%) for 48 hours, to facilitate dissection and analysis of the morphological changes of the individuals. Four stages of the intra-pupal development of L. eximia were observed: 1) Larva-pupa apolysis, which lasted 23 ± 1.08 h; 2) Cryptocephalic pupa, 5 ± 0.53 h; 3) Phanerocephalic pupa, 92 ± 1.94 h; and 4) Pharate adult: Transparents eyes, 125 ± 2.15 h; Yellow eyes, 23 ± 0.89 h; Pink eyes, 14 ± 0.73 h; and Red eyes, 20 ± 0.60 h. The pharate adult is completely formed after 296 hours and the emergence of the adult occurred after 302 ± 3.81 h. In addition, there were included the stage of pre-pupa, pupariation and the beginning of the adult stage, Imago and adult emergence. Each stage is described and compared with those described for Chrysomya albiceps (Calliphoridae). KEYWORDS: Immature insects, Metamorphosis, Forensic entomology, Postmortem interval.Desenvolvimento Intra-pupal de Lucilia eximia (Diptera: Calliphoridae) RESUMO No mundo existem poucos estudos sobre o desenvolvimento intrapupal de Diptera. Comumente, os estudos dos ciclos de vida das espécies de moscas são feitos eliminando o desenvolvimento embrionário que é muito importante, principalmente nas espécies de interesse forense. O principal objetivo foi o de analisar o desenvolvimento intrapupal de Lucilia eximia, descrevendo as mudanças morfológicas que ocorrem durante o desenvolvimento intrapupal. Foram analisados cerca de 1.600 espécimes cultivados em laboratório. Foram analisadas as fases de pré-pupa, pupa, o início da fase adulta, a imago e a emergência. Pré-pupas foram identificadas de acordo com a redução no tamanho e na alteração da coloração. Dez espécimes foram coletados a cada três horas (n=1.000), sem interrupção, até a emergência dos adultos. As pupas foram fixadas em etanol a 96%. Em seguida foram imersas em solução Carnoy por 24 horas e ácido fórmico a 5% por 48 horas para facilitar a dissecação e análises das alterações morfológicas. Foram determinados 4 estágios de desenvolvimento intrapupal. 1) Apólise larva-pupa com duração de 23 ± 1,08 h; 2) Pupa criptocefálica: 5 ± 0,53 h; 3) Pupa fanerocefálica: 92 ± 1,94 h e; 4) Adulto farado: olhos transparentes: 125 ± 2,15 h; olhos amarelos: 23 ± 0,89 h; olhos róseos: 14 ± 0,73 h e olhos vermelhos 20 ± 0,60 h. O a...

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Section: Discussioncontrasting

confidence: 89%

Section: Amazonicasupporting

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Intra-puparial Developmentmentioning

confidence: 99%

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Intra-puparial development of Lucilia eximia (Diptera, Calliphoridae)

2017

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“…It is not our aim to disregard the studies of Pujol-Luz and Barros-Cordeiro (2012), Defilippo et al (2013), Proença et al (2014), and Ma et al (2015). Indeed, we are aware of the wide confusion regarding the terminology and concepts related to intrapuparial development and particularly in the forensic entomology literature (we have probably also sometimes misused the terms) and therefore the reigning confusion is understandable.…”

mentioning

confidence: 99%

Resolving Confusion in the Use of Concepts and Terminology in Intrapuparial Development Studies of Cyclorrhaphous Diptera

Martín‐Vega¹,

Hall²

2016

J Med Entomol

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Effect of constant and fluctuating temperature on the intrapuparial development of Sarcophaga argyrostoma (Robineau‐Desvoidy, 1830; Diptera: Sarcophagidae)

2021

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This study aimes to investigate the effect of constant and fluctuating temperatures on starting times of developmental periods of insect species. For this purpose, Sarcophaga (Liopygia) argyrostoma (Robineau‐Desvoidy, 1830), a forensically important species has been chosen. Whether the constant (25°C) and fluctuating (25.2°C averaged over natural conditions) temperatures would make a difference on the timing of intrapuparial development of this species was investigated. First larval instars were placed in rearing cages until they reached post‐feeding third larval instar, which is considered the beginning of intrapuparial development. Relevant morphological changes were observed by hourly dissections. At fluctuating temperature treatment, the post‐feeding third instar larvae were transferred to containers under natural, open‐air conditions. Minimum and maximum starting times of 23 intrapuparial development periods were determined for both treatments. These times, medians, and standard deviations were compared by Mann–Whitney U tests. Accumulated degree hour values were also calculated for these periods. Regardless of temperature treatment, intrapuparial development periods were similar and adult emergence was observed at 289.5 ( ± 1.52) h under 25°C constant temperature and 319 ( ± 1.41) h under fluctuating temperature conditions. No statistical difference was found between the intrapuparial development of S. argyrostoma reared under constant and fluctuating temperatures, except “pupal‐adult apolysis” and “checkered pattern on the abdomen becoming distinct” periods. The use of the results obtained when determining the effect of constant and fluctuating temperatures on the starting times of developmental periods of this species in forensic entomology, is also important in transferring developmental biology data into the field of applied sciences.

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Intra-puparial development of the females of Chrysomya albiceps (Wiedemann) (Diptera, Calliphoridae)

Cited by 29 publications

References 19 publications

Intra-puparial development of Lucilia eximia (Diptera, Calliphoridae)

Intra-puparial development of Lucilia eximia (Diptera, Calliphoridae)

Resolving Confusion in the Use of Concepts and Terminology in Intrapuparial Development Studies of Cyclorrhaphous Diptera

Effect of constant and fluctuating temperature on the intrapuparial development of Sarcophaga argyrostoma (Robineau‐Desvoidy, 1830; Diptera: Sarcophagidae)

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