DsRed2 transient expression in Culex quinquefasciatus mosquitoes

Wilke, André Barretto Bruno; Scaife, Sarah; Alphey, Luke; Marrelli, Mauro Toledo

doi:10.1590/s0074-02762013000400023

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…Hence, it will be possible to study the function and regulation of the genes in different tissues using tissue specific promoters. It is noteworthy that baculovirus transduction could achieve maximum transgene expression at a low viral dose (1 × 10 5 PFU) compared to the gene expression at a high viral dose (1 × 10 7 PFU) by mosquito densoviruses (MDVs) 7 and to plasmid injection methods 40 . In contrast to MDVs 7,41 , baculovirus likely does not exhibit species or tissue barriers, so construction of a single recombinant baculovirus is sufficient to study gene regulation in various mosquito species.…”

Section: Discussionmentioning

confidence: 99%

Baculovirus as an efficient vector for gene delivery into mosquitoes

Naik

et al. 2018

Sci Rep

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Efficient gene delivery technologies play an essential role in the gene functional analyses that are necessary for basic and applied researches. Mosquitoes are ubiquitous insects, responsible for transmitting many deadly arboviruses causing millions of human deaths every year. The lack of efficient and flexible gene delivery strategies in mosquitoes are among the major hurdles for the study of mosquito biology and mosquito-pathogen interactions. We found that Autographa californica multiple nucleopolyhedrovirus (AcMNPV), the type baculovirus species, can efficiently transduce mosquito cells without viral propagation, allowing high level gene expression upon inducement by suitable promoters without obvious negative effects on cell propagation and viability. AcMNPV transduces into several mosquito cell types, efficiently than in commonly used mammalian cell lines and classical plasmid DNA transfection approaches. We demonstrated the application of this system by expressing influenza virus neuraminidase (NA) into mosquito hosts. Moreover, AcMNPV can transduce both larvae and adults of essentially all blood-sucking mosquito genera, resulting in bright fluorescence in insect bodies with little or no tissue barriers. Our experiments establish baculovirus as a convenient and powerful gene delivery vector in vitro and in vivo that will greatly benefit research into mosquito gene regulation, development and the study of mosquito-borne viruses.

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Section: Discussionmentioning

confidence: 99%

Baculovirus as an efficient vector for gene delivery into mosquitoes

Naik

et al. 2018

Sci Rep

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Transgenic Mosquitoes – Fact or Fiction?

Wilke

Beier

Benelli

2018

Trends in Parasitology

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Culex quinquefasciatus: status as a threat to island avifauna and options for genetic control

et al. 2021

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The avifauna endemic to islands is particularly susceptible to population declines and extinctions resulting from the introduction of non-native pathogens. Three pathogens of concern are the avian malaria parasites, the avian poxviruses, and West Nile virus—each of which can be transmitted by Culex quinquefasciatus, a highly adaptive and invasive mosquito. Culex quinquefasciatus has dramatically expanded its range in recent centuries and is now established throughout much of the tropics and sub-tropics, including on many islands that are remote from mainland landmasses and where this geographic separation historically protected island species from mosquito-borne diseases. The potential for ecological disruption by Cx. quinquefasciatus has been particularly striking in the Hawaiian Islands, where the introduction and transmission of avian malaria and avian poxvirus led to the extinction of several endemic bird species, with many more at risk. With Cx. quinquefasciatus now present in many insular communities and global trade and tourism increasing links between these areas, both to each other and to mainlands, there is growing concern that patterns of avian decline in Hawai‘i may be played out in other insular ecosystems. The implementation of traditional methods for Cx. quinquefasciatus control, including larval source management, is often impractical at large scale and when breeding sites are numerous and difficult to locate—typical issues associated with invasive species removal. One alternative approach would be the utilisation of genetic control methods, several of which have been successfully developed in other mosquitos such as Aedes aegypti and the malaria vector Anopheles gambiae. However, the development of similar tools for Cx. quinquefasciatus has been comparatively limited. Here we review the threat that Cx. quinquefasciatus poses as a vector of avian pathogens to island avifauna and discuss specific examples of at-risk bird populations on the islands of Hawai‘i, New Zealand and Galápagos. We also review the major options for the deployment of genetic control tools against Cx. quinquefasciatus, and discuss the current state of the field with a focus on radiation-based sterilisation, transgenic methods, and transinfections using the bacterial endosymbiont Wolbachia.

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DsRed2 transient expression in Culex quinquefasciatus mosquitoes

Cited by 3 publications

References 23 publications

Baculovirus as an efficient vector for gene delivery into mosquitoes

Baculovirus as an efficient vector for gene delivery into mosquitoes

Transgenic Mosquitoes – Fact or Fiction?

Culex quinquefasciatus: status as a threat to island avifauna and options for genetic control

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