Applications of the hexanic fraction of Agave sisalana Perrine ex Engelm (Asparagaceae): control of inflammation and pain screening

Dunder, Ricardo José; Luiz-Ferreira, Ânderson; Almeida, Ana Cristina Alves de; de-Faria, Felipe Meira; Takayama, Christiane; Socca, Eduardo Augusto Rabelo; Salvador, Marcos José; Mello, Gláucia C.; Santos, Catarina dos; Neto, Pedro de Oliva; Souza‐Brito, Alba Regina Monteiro

doi:10.1590/s0074-02762013000300002

Cited by 7 publications

(10 citation statements)

References 30 publications

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“…Hecogenin (Steroidal sapogenin) is the most ubiquitous glycoside isolated from leaves of various Agave genus species, has anti-inflammatory effects. It is traditionally used for immunomodulatory [45], anthelmintics [46], analgesic, anti-inflammatory activity [47], hepatoprotective, antiulcer activity [21] and antimicrobial activity [48]. There are very few studies showing the anti-inflammatory activity of hecogenin [47,49].…”

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confidence: 99%

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Anti-Inﬂammatory Potential of Hecogenin in Experimental Animals: Possible Involvement of Inflammatory Cytokines and Myeloperoxidase

Ingawale

Patel

2016

Drug Res (Stuttg)

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Hecogenin is a steroidal sapogenin plays important role in treatment of variety of inflammatory diseases. We have investigated the anti-inflammatory effects of Hecogenin (50 µg/animal) (HG), Fluticasone (50 µg/animal) (FC) and Hecogenin+Fluticasone (HG+FC) combination (25 µg/animal, each) on various inflammatory models. The anti-inflammatory effect of HG, FC and HG+FC combination was studied on % inhibition of dry weight of granuloma tissue, Δ ear weight, myeloperoxidase assay, serum pro-inflammatory cytokines, colon weight to length ratio, macroscopic lesions, adhesion score, diarrhoea score and histopathological analysis of ear and colon tissue on Cotton pellets induced granuloma in rats, Croton oil induced ear edema in mice and TNBS induced granuloma in rats. Topical administration of HG and its combination with FC showed significant decrease (p<0.001) in the % inhibition of dry weight of granuloma tissue, Δ ear weight, myeloperoxidase level, serum pro-inflammatory cytokines levels, colon weigh to length ratio as compared with Cotton pellets treated with acetone groups and Croton oil treated animals. Further histopathological analysis of ear tissue showed significant decrease in dermal thickness and epidermal hyperplasia and colon tissue showed reduction of edema, infiltration of inflammatory cells and normalization of crypt structure compared to DC animals. Thus, the findings of present study suggest the possible role of HG in the treatment of inflammation by reducing the dose of FC in combination with HG.

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“…There are very few studies showing the anti-inflammatory activity of hecogenin [47,49]. Dunder [47]. Peana et al, (1997) have studied the anti-inflammatory activity of aqueous extract of Agave americana and genins isolated from them on carrageenan induced edema in rats [50].…”

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Anti-Inﬂammatory Potential of Hecogenin in Experimental Animals: Possible Involvement of Inflammatory Cytokines and Myeloperoxidase

Ingawale

Patel

2016

Drug Res (Stuttg)

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“…Furthermore, the plant did not cause mortality in experimental groups, and it did not promote signs of toxicity such as changes in behaviour, ataxia, salivation, vomiting, diarrhoea, polyphagia, fever, weakness, tremors or convulsions (Viel et al, 2017). In addition, a lack of toxicity was observed when the hexane fraction obtained from the hydrolysed extract of A. sisalana was administered in an acute dose to rats and mice; the chemical high-performance liquid chromatography analyses of the hexane fraction of A. sisalana confirmed the presence of two steroidal sapogenins, hecogenin and tigogenin (Dunder et al, 2013).…”

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confidence: 99%

Preliminary phytochemical analysis and the effect of Agave sisalana on body weight and defensive behaviours in ovariectomized rats

Andrade¹,

Silva²,

Espinoza³

et al. 2017

J. Med. Plants Res.

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Agave sisalana is a plant belonging to the Agavaceae family. Sisal juice is constituted of steroidic saponins which are precursor molecules of many pharmacologically active steroids. These precursor molecules can act in brain structures that are related to the modulation of emotional disorders. They can act on energy metabolism or directly on the absorptive process of fats. The objective of this research was to identify the biocompounds present in A. sisalana juice, and to evaluate the effect of different extracts on the expression of defensive behaviours of rats in elevated plus maze and open field tests, and body weight, in a condition that corresponds to an induced menopause. Wistar rats were subjected to bilateral ovariectomy or to a sham surgical procedure under anaesthesia. Following surgery, they were treated gavage with sisal juice -concentrated crude juice (CCJ): 500 or 1000 mg/kg; dried extract (DE): 50, 100 or 200 mg/kg; dry mucilage extract (DME): 25, 50 or 100 mg/kg; and intermediate product (IP): 25, 50 or 100 mg/kg; or distilled water. The results showed the presence of coumarins, flavonoids, condensed tannins, free anthraquinones, alkaloids and saponins in the sisal juice. Both CCJ and DE (100 mg/kg) caused weight loss without alteration of defensive behaviours related to the manifestation of anxiety. As saponins were identified in DE in significant amounts, the observed effects were attributed to this component. Such findings point to A. sisalana as a plant that could potentially be used to treat weight gain during menopause.

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“…The isolation and structure determination of five steroidal saponins present in Agave sisalana has been reported to confirm the basis of chemical and phytochemical affirmation that this plant can be used in pharmacological tests [ 12 ]. There are numerous pharmacological treatments derived from saponins, such as antifungal, anti-allergic, anti-inflammatory, antibiotic, hepatoprotective, and antitumor activity [ 8 , 13 , 14 , 15 , 16 ]. Moreover, steroidal sapogenins, lipophilic portions of saponins, are the precursors of pharmacologically active steroid anti-inflammatory drugs present in glucocorticoids [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Toxicity and Anti-Inflammatory Effects of Agave sisalana Extract Derived from Agroindustrial Residue

Costa

Fracasso

Guarnier

et al. 2023

Plants

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Background: In several countries, the leaf juice of Agave sisalana (also known as sisal) is widely used topically, especially as an antiseptic, and orally for the treatment of different pathologies. However, in Brazil, which is the largest producer of Agave sisalana, its residue, which represents the majority of its weight, has been thrown away. For this reason, the determination of the pharmacological and toxicological potentials of sisal residue and its possible therapeutic use is seen as a way to contribute to the sustainable development and social promotion of the largest producer of sisal in Brazil, the interior of Bahia State, which is among the poorest areas in the country. Given the scarcity of available scientific studies on the pharmacological and toxicological properties of sisal residue juice, this study aimed to promote the acid hydrolysis of this juice to potentiate the anti-inflammatory effect already described in the literature. Furthermore, it aimed to evaluate the toxicological profile of the hydrolyzed extract (EAH) and to determine its acute toxicity, as well as its side effects on the reproductive aspects of rats. Method: The anti-inflammatory effect of EAH was evaluated in vitro using the induction of hemolysis by hypotonic solution and in vivo in rats using the carrageenan-induced paw edema test and the xylene-induced ear edema test. The acute toxicity, resulting from a single-dose administration, was investigated for some manifestation of toxic symptoms related to motor control and consciousness in rats. At a concentration of 100 mg/kg, by repeated doses, the reproductive toxicity effects of EAH in rats were assessed. Results: In vitro anti-inflammatory activity was positive using the human red blood cell membrane stabilization method. In both in vivo tests used to assess the anti-inflammatory activity, EAH (at three doses) significantly inhibited edema when compared to the control group. At a dose of 50 mg/kg, EAH exhibited a greater effect than indomethacin, a nonsteroidal anti-inflammatory drug with known activity. In vivo toxicological studies have shown that EAH does not present toxic effects when administered orally in a single dose, up to 1000 mg/kg. Finally, EAH promoted a gonadotoxic effect and increased the embryonic mortality rate after implantation. Conclusions: It is suggested that the anti-edematogenic effect of the acid hydrolysis extract from sisal juice is due to the high concentration of steroidal sapogenins. Therefore, this extract can be considered a potential new anti-inflammatory or even an important sapogenin source for the development of steroidal glucocorticoids. However, further studies are needed to elucidate the chemical composition of sisal juice. Regarding toxicology studies, EAH did not show cytotoxic and clastogenic potentials, but it presented a powerful reproductive toxic effect in rats.

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Applications of the hexanic fraction of Agave sisalana Perrine ex Engelm (Asparagaceae): control of inflammation and pain screening

Abstract: The present study evaluated the anti-inflammatory and analgesic properties of

Cited by 7 publications

References 30 publications

Anti-Inﬂammatory Potential of Hecogenin in Experimental Animals: Possible Involvement of Inflammatory Cytokines and Myeloperoxidase

Anti-Inﬂammatory Potential of Hecogenin in Experimental Animals: Possible Involvement of Inflammatory Cytokines and Myeloperoxidase

Preliminary phytochemical analysis and the effect of Agave sisalana on body weight and defensive behaviours in ovariectomized rats

Toxicity and Anti-Inflammatory Effects of Agave sisalana Extract Derived from Agroindustrial Residue

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