Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence

Dias, André Alves; Raze, Dominique; Lima, Cristiana Soares de; Marques, Maria Angela M.; Drobecq, Hervé; Debrie, Anne-Sophie; Ribeiro-Guimarães, Michelle Lopes; Biet, Franck; Pessolani, Maria Cristina Vidal

doi:10.1590/s0074-02762012000900025

Cited by 7 publications

(8 citation statements)

References 36 publications

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“…Hlp and HBHA are well known as extracellular matrixbinding proteins. While HBHA binds heparan sulfate (13,20), Hlp has been described as a multifunctional adhesin able to interact with laminin, hyaluronic acid, heparan sulfate, and collagen (14,53,(71)(72)(73). Previous studies have shown the in vivo expression of both proteins by M. leprae (62,71).…”

Section: Discussionmentioning

confidence: 99%

Interaction of Mycobacterium leprae with Human Airway Epithelial Cells: Adherence, Entry, Survival, and Identification of Potential Adhesins by Surface Proteome Analysis

et al. 2013

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f This study examined the in vitro interaction between Mycobacterium leprae, the causative agent of leprosy, and human alveolar and nasal epithelial cells, demonstrating that M. leprae can enter both cell types and that both are capable of sustaining bacterial survival. Moreover, delivery of M. leprae to the nasal septum of mice resulted in macrophage and epithelial cell infection in the lung tissue, sustaining the idea that the airways constitute an important M. leprae entry route into the human body. Since critical aspects in understanding the mechanisms of infection are the identification and characterization of the adhesins involved in pathogen-host cell interaction, the nude mouse-derived M. leprae cell surface-exposed proteome was studied to uncover potentially relevant adhesin candidates. A total of 279 cell surface-exposed proteins were identified based on selective biotinylation, streptavidin-affinity purification, and shotgun mass spectrometry; 11 of those proteins have been previously described as potential adhesins. In vitro assays with the recombinant forms of the histone-like protein (Hlp) and the heparin-binding hemagglutinin (HBHA), considered to be major mycobacterial adhesins, confirmed their capacity to promote bacterial attachment to epithelial cells. Taking our data together, they suggest that the airway epithelium may act as a reservoir and/or portal of entry for M. leprae in humans. Moreover, our report sheds light on the potentially critical adhesins involved in M. leprae-epithelial cell interaction that may be useful in designing more effective tools for leprosy control.

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Section: Discussionmentioning

confidence: 99%

Interaction of Mycobacterium leprae with Human Airway Epithelial Cells: Adherence, Entry, Survival, and Identification of Potential Adhesins by Surface Proteome Analysis

et al. 2013

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“…ATIIs express a variety of molecules that have been shown to participate in receptor-mediated endocytosis following recognition of M.tb antigens, such as integrins [6], carbohydrate receptors and extracellular matrix (ECM) components [40][41] [42;43], and pattern recognition receptors (PRRs) [6]. Integrins, including CD51 (vitronectin) and CD29 (β1 integrin), have been shown to synergize and be involved in M.tb invasion [6].…”

Section: Mtb Binding and Invasion Of The Alveolar Epitheliummentioning

confidence: 99%

“…Surface carbohydrate receptors such as heparin, heparin sulfate, and hyaluronic acid bound to extracellular matrix (ECM) molecules may also allow M.tb access into ATIIs through multiple routes thereby enhancing their susceptibility to pathogen invasion [6;43]. For example, laminin-binding protein (LBP) was first implicated in host cell binding during infection with M. leprae and has since been shown to facilitate M.tb binding to the alveolar epithelium through hyaluronic acid, laminin and collagen [43].…”

Section: Mtb Binding and Invasion Of The Alveolar Epitheliummentioning

confidence: 99%

“…For example, laminin-binding protein (LBP) was first implicated in host cell binding during infection with M. leprae and has since been shown to facilitate M.tb binding to the alveolar epithelium through hyaluronic acid, laminin and collagen [43]. …”

Section: Mtb Binding and Invasion Of The Alveolar Epitheliummentioning

confidence: 99%

“…In vivo inhibition of HBHA or mutated HBHA- M.tb strains show fewer M.tb bacilli located outside the lung compartment supporting a role for this M.tb antigen in dissemination [21]. In contrast, HBHA does not bind to phagocytes, suggesting a unique relationship between this antigen and ATIIs [43]. …”

Section: Mtb Binding and Invasion Of The Alveolar Epitheliummentioning

confidence: 99%

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Alveolar Epithelial Cells in <b><i>Mycobacterium tuberculosis</i></b> Infection: Active Players or Innocent Bystanders?

Scordo

Knoell²,

Torrelles

2015

J Innate Immun

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Tuberculosis (TB) is a disease that kills one person every 18 s. TB remains a global threat due to the emergence of drug-resistant Mycobacterium tuberculosis (M.tb) strains and the lack of an efficient vaccine. The ability of M.tb to persist in latency, evade recognition following seroconversion, and establish resistance in vulnerable populations warrants closer examination. Past and current research has primarily focused on examination of the role of alveolar macrophages and dendritic cells during M.tb infection, which are critical in the establishment of the host response during infection. However, emerging evidence indicates that the alveolar epithelium is a harbor for M.tb and critical during progression to active disease. Here we evaluate the relatively unexplored role of the alveolar epithelium as a reservoir and also its capacity to secrete soluble mediators upon M.tb exposure, which influence the extent of infection. We further discuss how the M.tb-alveolar epithelium interaction instigates cell-to-cell crosstalk that regulates the immune balance between a proinflammatory and an immunoregulatory state, thereby prohibiting or allowing the establishment of infection. We propose that consideration of alveolar epithelia provides a more comprehensive understanding of the lung environment in vivo in the context of host defense against M.tb.

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Autolysin mediated adherence of Staphylococcus aureus with Fibronectin, Gelatin and Heparin

Porayath

Suresh

Biswas

et al. 2018

International Journal of Biological Macromolecules

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Major autolysin (Atl) of Staphylococcus aureus is a cell surface associated peptidoglycan hydrolase with amidase and glucosaminidase domains. Atl enzymes (amidase and glucosaminidase) are known to participate in biofilm formation and also can bind with host matrices. Earlier studies demonstrated the binding of Atlwithfibronectin, thrombospondin 1, vitronectin and heat shock cognate protein Hsc70. Here, we have shown, Atl mediates attachment of S.aureus to heparin and gelatine as well. The atl mutant strain demonstrated around 2.5 fold decreased adherence with fibronectin, gelatin and heparin coated microtiter plates. The microscopic studies confirmed the reduced binding of atl mutant with them compared to its parental wild type and complemented mutant strains. Amidase and glucosaminidase were expressed as N -terminal histidine tagged proteins from Escherichia coli , purified and refolded. We found refolded amidase bind with fibronectin, gelatin and heparin; whereas refolded glucosaminidase binds with only fibronectin and heparin but not gelatin. These results reemphasize Atl as one of the crucial proteins from Staphylococcus that facilitate their binding with multiple host cellular components during colonization and infection.

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Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence

Cited by 7 publications

References 36 publications

Interaction of Mycobacterium leprae with Human Airway Epithelial Cells: Adherence, Entry, Survival, and Identification of Potential Adhesins by Surface Proteome Analysis

Interaction of Mycobacterium leprae with Human Airway Epithelial Cells: Adherence, Entry, Survival, and Identification of Potential Adhesins by Surface Proteome Analysis

Alveolar Epithelial Cells in <b><i>Mycobacterium tuberculosis</i></b> Infection: Active Players or Innocent Bystanders?

Autolysin mediated adherence of Staphylococcus aureus with Fibronectin, Gelatin and Heparin

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