Genotyping of gastroenteric viruses in hospitalised children: first report of norovirus GII.21 in Brazil

Ferreira, M.S.R.; Garcia, Rita de Casia Cubel; Xavier, Maria da Penha Trindade Pinheiro; Ribeiro, Rubia Lane; Assis, Rosane Maria Santos de; Mota, Maria do Ceu M.S; Leite, José Paulo Gagliardi; Miagostovich, Marize Pereira; Oliveira, Solange Artimos de

doi:10.1590/s0074-02762012000800017

Cited by 17 publications

(19 citation statements)

References 24 publications

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“…The prevalence of GII.6 ,the second most predominant cause of gastroenteritis among our study participants ,was similar to reports in several countries including Brazil[58], Japan[59], Africa[60] and Finland[61]. GII.21, previously reported in Brazil[62], has been described as a recombinant product between GII.4/2006b and GII.18 strains[63]. In our study, this genotype was similar to the Salisbury150/2011/United States GII.21.…”

Section: Discussionsupporting

confidence: 90%

Clinical and epidemiological characteristics of norovirus gastroenteritis among hospitalized children in Lebanon

Melhem

Zaraket

Kreidieh

et al. 2016

WJG

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AIMTo assess the burden of norovirus (NoV) and to determine the diversity of circulating strains among hospitalized children in Lebanon.METHODSStool samples were collected from children presenting with acute gastroenteritis to six major hospitals in Lebanon. A total of 739 eligible stool samples, testing negative for diarrhea caused by rotavirus as a possible viral pathogen, were collected between January 2011 and June 2013. A standardized questionnaire including demographic, epidemiological and clinical observations was used at the time of hospitalization of children presenting with diarrhea. Viral RNA was extracted from stool samples followed by reverse transcription polymerase chain reaction and nucleotide sequencing of a fragment of the viral protein 1 capsid gene. Multiple sequence alignments were carried out and phylogenetic trees were constructed using the MEGA 6 software.RESULTSOverall, 11.2% of stool samples collected from children aged < 5 years tested positive for NoV genogroups I (GI) and II (GII). GII accounted for 10.6% of the gastroenteritis cases with only five samples being positive for GI (0.7%). The majority of hospitalized children showed symptoms of diarrhea, dehydration, vomiting and fever. Upon sequencing of positive samples and based on their clustering in the phylogenetic tree, 4/5 of GI gastroenteritis cases were designated GI.3 and one case as GI.4. GII.4 was predominantly detected in stool of our study participants (68%). We report a JB-15/KOR/2008 GII.4 Apeldoorn 2008-like variant strain circulating in 2011; this strain was replaced between 2012 and 2013 by a variant sharing homology with the Sydney/NSW0514/2012/AUS GII.4 Sydney 2012 and Sydney 2012/FRA GII.4 strains. We also report the co-circulation of non-GII.4 genotypes among hospitalized children. Our data show that NoV gastroenteritis can occur throughout the year with the highest number of cases detected during the hot months.CONCLUSIONThe majority of NoV-associated viral gastroenteritis cases among our participants are attributable to GII.4, which is compatible with results reported worldwide.

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Section: Discussionsupporting

confidence: 90%

Clinical and epidemiological characteristics of norovirus gastroenteritis among hospitalized children in Lebanon

Melhem

Zaraket

Kreidieh

et al. 2016

WJG

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“…The individual with GII.21 in the current study was an elderly adult and, taken together with the finding of Ferreira et al [], it appears GII.21 norovirus can occur in both children and adults but is rare. The current study is one of the first to identify the norovirus recombinant form GII.b/GII.21 although Ferreira et al [] note that a previous report by Chhabra et al [] mistakenly classified a GII.b/GII.21 as GII.b/GII.18.…”

Section: Discussionsupporting

confidence: 84%

“…There appears to be little information available on GII.21 norovirus (Kroneman classification) apart from a report of GII.21 (ORF 2) in one child with gastroenteritis in Brazil [Ferreira et al, ] and another report by Perez‐Sautu et al [] who detected GII.21 (ORF 2) in a river catchment area in Spain. The individual with GII.21 in the current study was an elderly adult and, taken together with the finding of Ferreira et al [], it appears GII.21 norovirus can occur in both children and adults but is rare. The current study is one of the first to identify the norovirus recombinant form GII.b/GII.21 although Ferreira et al [] note that a previous report by Chhabra et al [] mistakenly classified a GII.b/GII.21 as GII.b/GII.18.…”

Section: Discussionmentioning

confidence: 99%

Altered patterns of norovirus GII.b recombinant forms in gastroenteritis outbreaks in victoria, Australia, 2002–2005 compared to 2006–2011

Bruggink

Marshall

2013

Journal of Medical Virology

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GII.b norovirus is an obligatory recombinant with an open reading frame (ORF) 1 comprising the GII.b genotype and the ORF 2 region corresponding to one of a number of other norovirus genotypes. GII.b is the second most common genotype after GII.4 associated with gastroenteritis outbreaks in Victoria, Australia. The study involved norovirus testing of 14,186 specimens from 2,743 Victorian gastroenteritis outbreaks in the period 2002-2011. The noroviruses identified were further characterized by nucleotide sequencing and phylogenetic analysis. In the period 2002-2005, 21 GII.b norovirus outbreaks were identified, with the GII.b ORF 2 recombinant genotypes comprising GII.1 (47.6%), GII.3 (47.6%), and GII.13 (4.8%). For the period 2006-2011, 56 GII.b norovirus outbreaks were identified. In 51 of these, the ORF 2 genotype was identified, and comprised GII.1 (2.0%), GII.3 (94.1%), GII.13 (2.0%), and GII.21 (2.0%). GII.b norovirus outbreaks could occur in a range of settings involving individuals with a broad range of ages. However, GII.b/GII.3 norovirus tended to occur in a younger demographic and all outbreaks involving specifically children's settings had the GII.b/GII.3 genotype. Nucleotide sequencing studies demonstrated major changes in both the ORF 1 and ORF 2 regions of the GII.b/GII.3 noroviruses but not in the GII.b/GII.1 and GII.b/GII.13 noroviruses when the noroviruses for the two time periods 2002-2005 and 2006-2011 were compared. The findings suggest nucleotide changes in the pre-existing GII.b/GII.3 noroviruses resulted in a more virulent form.

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“…In Brazil, NoV was first reported in 1993 and since then, the presence of GI and GII has been reported in several regions of the country, in the North, Midwest, Southeast and Northeast (4,5,6,7,8,9) . NoV is an etiologic agent frequently reported in gastrointestinal disorders in Brazil and, especially in the Northeast, few studies have been developed for this purpose.…”

Section: Introductionmentioning

confidence: 99%

Acute Gastroenteritis Associated With Norovirus GII.4 Variants

Paula

Sardi

Tigre

et al. 2018

Arq. Gastroenterol.

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BACKGROUND: Norovirus (NoV) is an important etiologic agent of acute gastroenteritis and infects individuals of all ages, especially children in Brazil and worldwide. NoV GII.4 was the most prevalent genotype worldwide because of your extensive genetic diversity. In Brazil, especially in the Northeast, few studies have been developed for identify and molecularly characterize NoV. OBJECTIVE: The present study aimed to detect and describe the molecular epidemiology of NoV associated with acute gastroenteritis. METHODS: The viral RNA extracted from stool samples were subjected to Nested RT-PCR and the genotypes were determined by nucleotide sequences analysis. In total, 278 stool samples assisted at Aliança Hospital in the city of Salvador, with acute gastroenteritis were examined, between March 2009 and July 2012. RESULTS: A high NoV rate (54.2%) was identified in children under 5 years of age. We detected the circulation of different NoV GII.4 variants in Salvador, during the study period as Den Haag 2006b, New Orleans 2009 and Sydney 2012. CONCLUSION: These findings reinforce the need to study the molecular epidemiology of NoV infections in acute gastroenteritis.

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Genotyping of gastroenteric viruses in hospitalised children: first report of norovirus GII.21 in Brazil

Cited by 17 publications

References 24 publications

Clinical and epidemiological characteristics of norovirus gastroenteritis among hospitalized children in Lebanon

Clinical and epidemiological characteristics of norovirus gastroenteritis among hospitalized children in Lebanon

Altered patterns of norovirus GII.b recombinant forms in gastroenteritis outbreaks in victoria, Australia, 2002–2005 compared to 2006–2011

Acute Gastroenteritis Associated With Norovirus GII.4 Variants

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