In vitro antimicrobial assessment of Cuban propolis extracts

Monzote, Lianet; Cuesta‐Rubio, Osmany; Fernández, Mercedes Campo; Hernández, Ingrid Márquez; Fraga, Jorge; Pérez, Kleich; Kerstens, Monique; Maes, Louis; Cos, Paul

doi:10.1590/s0074-02762012000800003

Cited by 49 publications

(35 citation statements)

References 35 publications

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“…The amounts of nemorosone in the propolis extracts (Table 1) were estimated by subtracting the nemorosone's absorbance from the absorbance of the nemorosone plus propolis extracts mixture, and then interpolating the values into the nemorosone's calibration curve. The nemorosone concentrations were 6.96, 6.01, 2.36, and 17.12 lM for N-1, N-3, N-12, and N-17 extracts, respectively, values close similar to those previously reported for the same extracts using high-performance liquid chromatography with photodiode array detector (HPLC-PDA) and HPLC with electrospray ionization mass spectrometer (HPLC-ESI/MS) techniques [17].…”

Section: Estimation Of Nemorosone's Content In the Propolis Samplesupporting

confidence: 87%

“…Therefore, mitochondrial uncoupling, here described for the first time in Cuban propolis, is potentially involved in the toxicity of nemorosone-containing propolis on normal and cancer cells, since the effects were close similar to the nemorosone effects on isolated mitochondria [13]. Therefore, nemorosone may be the main agent responsible for the extract's cytotoxic and antiparasitic actions of Cuban propolis [16,17]. In this regard, it has been demonstrated that mitochondrial uncouplers induce death in several cancer cell types [26][27][28].…”

Section: Discussionmentioning

confidence: 74%

“…We recently reported that nemorosone and its chemical analogues guttiferone A and clusianone presented strong cytotoxic effects mediated by mitochondrial uncoupling [13][14][15]. Therefore, it can be hypothesize that the http cytotoxic and antiparasitic effects of the brown Cuban propolis reported elsewhere [16,17] may be related to their nemorosone's content and mediated by a mitotoxic uncoupling effect.…”

Section: Introductionmentioning

confidence: 98%

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The cytotoxic effects of brown Cuban propolis depend on the nemorosone content and may be mediated by mitochondrial uncoupling

Pardo-Andreu

Reis

Dalalio

et al. 2015

Chemico-Biological Interactions

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Three main types of Cuban propolis directly related to their secondary metabolite composition have been identified: brown, red and yellow propolis; the former is majoritarian and is characterized by the presence of nemorosone. In this study, brown Cuban propolis extracts were found cytotoxic against HepG2 cells and primary rat hepatocytes, in close association with the nemorosone contents. In mitochondria isolated from rat liver the extracts displayed uncoupling activity, which was demonstrated by the increase in succinate-supported state 4 respiration rates, dissipation of mitochondrial membrane potential, Ca(2+) release from Ca(2+)-loaded mitochondria, and a marked ATP depletion. As in cells, the degree of such mitotoxic events was closely correlated to the nemorosone content. The propolis extracts that do not contain nemorosone were neither cytotoxic nor mitotoxic, except R-29, whose detrimental effect upon cells and mitochondria could be mediated by its isoflavonoids and chalcones components, well known mitochondrial uncouplers. Our results at least partly unravel the cytotoxic mechanism of Cuban propolis, particularly regarding brown propolis, and raise concerns about the toxicological implication of Cuban propolis consumption.

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Section: Estimation Of Nemorosone's Content In the Propolis Samplesupporting

confidence: 87%

Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 98%

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The cytotoxic effects of brown Cuban propolis depend on the nemorosone content and may be mediated by mitochondrial uncoupling

Pardo-Andreu

Reis

Dalalio

et al. 2015

Chemico-Biological Interactions

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“…Significant variability of the chemical composition of propolis samples and hence their biological activity has been also found by other authors [30,31]. Monzote and coworkers [32] stated lack of antifungal activity in the group of 20 Cuban propolis extracts. Thus using propolis as a therapeutic agent would require special attention.…”

Section: Discussionsupporting

confidence: 61%

Essential Oils, Silver Nanoparticles and Propolis as Alternative Agents Against Fluconazole Resistant Candida albicans, Candida glabrata and Candida krusei Clinical Isolates

et al. 2014

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Development of effective and safe therapeutic treatment of fungal infections remains one of the major challenge for modern medicine. The aim of presented investigation was to analyze the in vitro antifungal activity of selected essential oils, ethanolic extracts of propolis and silver nanoparticles dropped on TiO 2 against azole-resistant C. albicans (n = 20), C. glabrata (n = 14) and C. krusei (n = 10) clinical isolates. Among tested essential oils, the highest activity has definitely been found in the case of the oil isolated from the bark of Cinnamomum cassia, with MIC and MFC values for all tested strains in the range of 0.0006-0.0097 % (v/v) and 0.0012-0.019 % (v/v), respectively. High activity was also observed for the Lemon, Basil, Thyme, Geranium and Clove (from buds) essential oils. Significant differences in fungicidal activity have been observed in the case of four tested propolis samples. Only one of them revealed high activity, with MFC values in the range from 0.156 to 1.25 % (v/v). Satisfactory fungicidal activity, against C. albicans and C. glabrata isolates, was also observed in the case of silver nanoparticles, however C. krusei isolates were mostly resistant. We also revealed that constituents of most of essential oils and propolis as well as silver nanoparticles are not substrates for drug transporters, which belong to the most important factors affecting resistance of Candida spp. clinical isolates to many of conventional antimycotics. To conclude, the results of our investigation revealed that essential oils, propolis and silver nanoparticles represent high potential for controlling and prevention candidiasis.

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“…Embora muitos estudos relatem a eficiência da própolis e de óleos essenciais no controle de fungos e outros microorganismos (Fernandes et al, 2007;Probst et al, 2011;Monzote et al, 2012), há casos em que essa eficiência não é confirmada. Marini et al (2012) A divergência observada em alguns resultados obtidos com a própolis deve-se em parte a sua origem.…”

Section: Resultsunclassified

Atividade biológica in vitro de própolis e óleos essenciais sobre o fungo Colletotrichum musae isolado de bananeira (Musa spp.)

Barbosa

Vieira

Teixeira

2015

Rev. bras. plantas med.

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RESUMO:No Brasil existem várias doenças fúngicas que acometem a bananeira. Destas, pode-se citar a antracnose, responsável por grandes prejuízos à cultura, cujo agente causal é o fungo Colletotrichum musae. A principal forma de controle dessa enfermidade é através da aplicação de fungicidas a base de tiabendazol ou tiofanato metílico. Esse manejo, embora eficiente, favorece o desenvolvimento de resistência do patógeno, causa danos ao ambiente e ao produtor, deixando ainda resíduos nos frutos. Esses fatores têm favorecido a busca por substâncias alternativas com capacidade de controlar o fungo e que não sejam nocivas ao ambiente e, principalmente, que sejam seguras ao consumidor final. Dentre as opções, surge o interesse pelo uso de certos óleos essenciais e da própolis, ambos conhecidos por possuírem propriedades fungicidas. O presente trabalho foi desenvolvido com o objetivo de determinar o potencial fungitóxico "in vitro" da própolis e dos óleos essenciais de palmarosa (Cymbopogon martinii), de teatree (Melaleuca alternifolia), de cravo (Eugenia caryophyllata), e de eucalipto (Corymbia citriodora), sobre Colletotrichum musae. O desenvolvimento experimental consistiu em adicionar inóculos fúngicos de 5 mm, obtidos a partir de colônias puras, ao meio de cultura BDA (batata-dextrose-ágar) acrescido das referidas substâncias em diferentes concentrações (0, 25, 50, 75, 100 e 125 µL/L). Paralelo aos tratamentos realizou-se teste com o fungicida padrão para comparações das médias. A eficiência das substâncias sobre o fungo foi determinada através das avaliações do crescimento micelial das colônias (média de duas medidas diametralmente opostas). Os valores de crescimento micelial obtidos foram utilizados também para o cálculo do índice de velocidade de crescimento micelial. O delineamento experimental utilizado foi inteiramente casualizado em esquema fatorial 5 x 6 + 1, (cinco substâncias em seis concentrações + fungicida), com cinco repetições. Os óleos de tea tree, cravo e palmarosa foram eficientes no controle do fungo Colletotrichum musae não diferindo do fungicida a partir da dose de 50 µL/L em todas as avaliações, apresentando potencial para controle em cultivos orgânicos ou em sistemas de manejo integrado. Palavras-chave:Produção orgânica, Fungos, Desenvolvimento sustentável, Própolis. ABSTRACT:In vitro biological activity of propolis and essential oils on the fungus Colletotrichum musae isolated from banana Musa spp. In Brazil there are several fungi that cause diseases on banana plants. These include the "anthracnose", which is responsible for major crop losses and whose causative agent is the fungus Colletotrichum musae. The main way to control this disease is through the application of fungicides based on thiabendazole and thiophanate-methyl. Although this management is effective, it favors the development of pathogen resistance, which causes damage to the environment and producer and also leaves residues in fruits. These factors have encouraged the search for alternative substances to control the fungus an...

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In vitro antimicrobial assessment of Cuban propolis extracts

Cited by 49 publications

References 35 publications

The cytotoxic effects of brown Cuban propolis depend on the nemorosone content and may be mediated by mitochondrial uncoupling

The cytotoxic effects of brown Cuban propolis depend on the nemorosone content and may be mediated by mitochondrial uncoupling

Essential Oils, Silver Nanoparticles and Propolis as Alternative Agents Against Fluconazole Resistant Candida albicans, Candida glabrata and Candida krusei Clinical Isolates

Atividade biológica in vitro de própolis e óleos essenciais sobre o fungo Colletotrichum musae isolado de bananeira (Musa spp.)

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