Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil

Matsumoto, Cristianne Kayoko; Chimara, Érica; Ramos, Jesus Pais; Campos, Carlos Eduardo Dias; Caldas, Paulo César de Souza; Lima, Karla Valéria Batista; Lopes, Maria Luíza; Duarte, Rafael Silva; Leão, Sylvia Cardoso

doi:10.1590/s0074-02762012000800002

Cited by 9 publications

(4 citation statements)

References 20 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The analysis of 100 colonies from the 10 th passage plate of each isolate by amplification of trfA1 gene from pMAB01 by PCR (PCR- trfA ) showed that the plasmid was stably maintained in isolates B52 [1] and IAL 042 [29], while 2% of the INCQS 00594 isolate’s colonies lost the plasmid. Plasmid cure was demonstrated by PFGE-DraI ( Figure 5 – lane 6).…”

Section: Resultsmentioning

confidence: 99%

“…The stability of pMAB01 plasmid was tested using three isolates, INCQS 00594, B52 [1] and IAL 042 [29], according to the protocol described by da Silva Rabello et al [63] with minor modifications. A single colony of each isolate was cultivated in 10 ml of LB broth, at 37°C, in a shaker, at 180 rpm, until the optical density at 600 nm reached 0.6 to 0.8.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The Detection and Sequencing of a Broad-Host-Range Conjugative IncP-1β Plasmid in an Epidemic Strain of Mycobacterium abscessus subsp. bolletii

et al. 2013

Self Cite

View full text Add to dashboard Cite

BackgroundAn extended outbreak of mycobacterial surgical infections occurred in Brazil during 2004–2008. Most infections were caused by a single strain of Mycobacterium abscessus subsp. bolletii, which was characterized by a specific rpoB sequevar and two highly similar pulsed-field gel electrophoresis (PFGE) patterns differentiated by the presence of a ∼50 kb band. The nature of this band was investigated.Methodology/Principal FindingsGenomic sequencing of the prototype outbreak isolate INCQS 00594 using the SOLiD platform demonstrated the presence of a 56,264-bp circular plasmid, designated pMAB01. Identity matrices, genetic distances and phylogeny analyses indicated that pMAB01 belongs to the broad-host-range plasmid subgroup IncP-1β and is highly related to BRA100, pJP4, pAKD33 and pB10. The presence of pMAB01-derived sequences in 41 M. abscessus subsp. bolletii isolates was evaluated using PCR, PFGE and Southern blot hybridization. Sixteen of the 41 isolates showed the presence of the plasmid. The plasmid was visualized as a ∼50-kb band using PFGE and Southern blot hybridization in 12 isolates. The remaining 25 isolates did not exhibit any evidence of this plasmid. The plasmid was successfully transferred to Escherichia coli by conjugation and transformation. Lateral transfer of pMAB01 to the high efficient plasmid transformation strain Mycobacterium smegmatis mc2155 could not be demonstrated.Conclusions/SignificanceThe occurrence of a broad-host-range IncP-1β plasmid in mycobacteria is reported for the first time. Thus, genetic exchange could result in the emergence of specific strains that might be better adapted to cause human disease.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The Detection and Sequencing of a Broad-Host-Range Conjugative IncP-1β Plasmid in an Epidemic Strain of Mycobacterium abscessus subsp. bolletii

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Forty-eight isolates from patients not related to outbreaks were included for comparison and calculation of the discriminatory power of the MLST scheme. These isolates were obtained from respiratory (30), ophthalmological (3), and injection abscess (1) samples between 2002 and 2013 in different states from Brazil (see Table SA1 (10). Isolated colonies from the same isolates were retyped by PFGE for this work, confirming the existence of three different PFGE patterns among the outbreak isolates.…”

Section: Mycobacterial Isolatesmentioning

confidence: 54%

Multilocus Sequence Typing Scheme versus Pulsed-Field Gel Electrophoresis for Typing Mycobacterium abscessus Isolates

et al. 2014

Self Cite

View full text Add to dashboard Cite

hOutbreaks of infections by rapidly growing mycobacteria following invasive procedures, such as ophthalmological, laparoscopic, arthroscopic, plastic, and cardiac surgeries, mesotherapy, and vaccination, have been detected in Brazil since 1998. Members of the Mycobacterium chelonae-Mycobacterium abscessus group have caused most of these outbreaks. As part of an epidemiological investigation, the isolates were typed by pulsed-field gel electrophoresis (PFGE). In this project, we performed a large-scale comparison of PFGE profiles with the results of a recently developed multilocus sequence typing (MLST) scheme for M. abscessus. Ninety-three isolates were analyzed, with 40 M. abscessus subsp. abscessus isolates, 47 M. abscessus subsp. bolletii isolates, and six isolates with no assigned subspecies. Forty-five isolates were obtained during five outbreaks, and 48 were sporadic isolates that were not associated with outbreaks. For MLST, seven housekeeping genes (argH, cya, glpK, gnd, murC, pta, and purH) were sequenced, and each isolate was assigned a sequence type (ST) from the combination of obtained alleles. The PFGE patterns of DraI-digested DNA were compared with the MLST results. All isolates were analyzable by both methods. Isolates from monoclonal outbreaks showed unique STs and indistinguishable or very similar PFGE patterns. Thirty-three STs and 49 unique PFGE patterns were identified among the 93 isolates. The Simpson's index of diversity values for MLST and PFGE were 0.69 and 0.93, respectively, for M. abscessus subsp. abscessus and 0.96 and 0.97, respectively, for M. abscessus subsp. bolletii. In conclusion, the MLST scheme showed 100% typeability and grouped monoclonal outbreak isolates in agreement with PFGE, but it was less discriminative than PFGE for M. abscessus.

show abstract

“…Forty-eight isolates from patients not related to outbreaks were included for comparison and calculation of the discriminatory power of the MLST scheme. These isolates were obtained from respiratory (30), ophthalmological (3), and injection abscess (1) samples between 2002 and 2013 in different states from Brazil (see Table SA1 in the supplemental material). Type strains M. abscessus ATCC 19977, M. massiliense CCUG 48898, and M. bolletii CCUG 50184 were used for comparison.…”

Section: Mycobacterial Isolatesmentioning

confidence: 99%

Benznidazole: Hero or villain of cellular immune response in chronic Chagas disease patients?

et al. 2021

View full text Add to dashboard Cite

Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil

Cited by 9 publications

References 20 publications

The Detection and Sequencing of a Broad-Host-Range Conjugative IncP-1β Plasmid in an Epidemic Strain of Mycobacterium abscessus subsp. bolletii

The Detection and Sequencing of a Broad-Host-Range Conjugative IncP-1β Plasmid in an Epidemic Strain of Mycobacterium abscessus subsp. bolletii

Multilocus Sequence Typing Scheme versus Pulsed-Field Gel Electrophoresis for Typing Mycobacterium abscessus Isolates

Benznidazole: Hero or villain of cellular immune response in chronic Chagas disease patients?

Contact Info

Product

Resources

About