Polymerase chain reaction for the evaluation of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil

Carvalho, Gabriel Costa de; Marques, Letícia Helena dos Santos; Gomes, Luciana I.; Rabello, Ana; Ribeiro, Luíz Cláudio; Scopel, Kezia K.G.; Tibiriçá, Sandra Helena Cerrato; Coimbra, Elaine Soares; Abramo, Clarice

doi:10.1590/s0074-02762012000700010

Cited by 16 publications

(11 citation statements)

References 15 publications

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“…For example, while the development of new technologies aimed at detecting malaria infections in areas of declining prevalence points to the need for improved operational approaches (Okell et al, 2012;Tietje et al, 2014), the current efforts to revisit prevalence data for Schistosoma mansoni and Schistosoma japonicum that are so far mainly based on the Kato-Katz thick smear technique (Katz et al, 1972), underlines the need for improved test sensitivity. In this connection, approaches, such as the polymerase chain reaction (PCR) (Carvalho et al, 2012), the loop-mediated isothermal amplification technique (LAMP) (Xu et al, 2010) and the determination of circulating antigens (van Lieshout et al, 2000) offer unprecedented opportunities. Indeed, the move to identify and evaluate highly sensitive diagnostics has become increasingly necessary as the prevalence and intensity of schistosome infections continues to decline the world over.…”

Section: Tools For Sustained Progressmentioning

confidence: 99%

Surveillance and response: Tools and approaches for the elimination stage of neglected tropical diseases

et al. 2015

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Section: Tools For Sustained Progressmentioning

confidence: 99%

Surveillance and response: Tools and approaches for the elimination stage of neglected tropical diseases

et al. 2015

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“…Of 824 schoolchildren in the two schools of Abis 4 and 8 villages participating in the study, 15 (1.8%) tested positive. Twelve (80%) of the detected cases had a light infection, two had a moderate infection and one case had a heavy infection.…”

Section: Prevalence and Intensity Of S Mansoni Infection By Kato-katzmentioning

confidence: 99%

Detection of low‐intensity Schistosoma mansoni infection by Percoll sedimentation and real‐time PCR techniques in a low‐endemicity Egyptian setting

Allam

Farag

Zaki

et al. 2015

Tropical Med Int Health

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Abstractobjective To evaluate the performance of Percoll sedimentation and real-time polymerase chain reaction (PCR) for the detection of S. mansoni cases previously tested as negative by Kato-Katz technique in two low-endemic areas in Alexandria, Egypt, Abis 4 and 8 villages.methods Stool samples of 824 primary schoolchildren were examined by Kato-Katz technique (three slides of 41.7 mg each). After obtaining the results of this survey, stool samples were recollected from a subset of 150 students, who gave negative results after Kato-Katz. These samples were microscopically examined after the concentration with Percoll technique. Part of the 150 negative stool samples and five positive samples (used as controls) were kept at À20°C and further processed by SYBR Green PCR.results Prevalence of S. mansoni infection as determined by three Kato-Katz thick smears was 1.82% (15 cases). Three more cases tested positive by Percoll sedimentation among the 150 samples that were negative by Kato-Katz. Specific amplification by SYBR Green PCR was noted in all positive controls and in three cases of Kato-Katz-negative samples, two of which were also positive by Percoll.conclusion Percoll sedimentation and SYBR Green PCR proved useful in detecting low-intensity S. mansoni infections in low-endemicity areas in Egypt.

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“…Furthermore, nucleic acid-based detection has been shown to be superior in identifying different schistosomes in cases of low-grade infections. The rate of positivity is dramatically higher than that when using the Kato-Katz method and miracidium hatching [ 8 , 12 , 18 , 28 – 30 ]. Deoxyribonucleic acid (DNA)-based assays remarkably reduce the false-negative rate and effectively monitor potential exposure to schistosomiasis.…”

Section: Introductionmentioning

confidence: 99%

Nucleic acid detection in the diagnosis and prevention of schistosomiasis

Song

Xie

et al. 2016

Infect Dis Poverty

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Schistosomiasis is an important zoonotic parasitic disease that causes serious harms to humans and animals. Surveillance and diagnosis play key roles in schistosomiasis control, however, current techniques for surveillance and diagnosis of the disease have limitations. As genome data for parasites are increasing, novel techniques for detection incorporating nucleotide sequences are receiving widespread attention. These sensitive, specific, and rapid detection methods are particularly important in the diagnosis of low-grade and early infections, and may prove to have clinical significance. This paper reviews the progress of nucleic acid detection in the diagnosis and prevention of schistosomiasis, including such aspects as the selection of target genes, and development and application of nucleic acid detection methods.Electronic supplementary materialThe online version of this article (doi:10.1186/s40249-016-0116-y) contains supplementary material, which is available to authorized users.

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Polymerase chain reaction for the evaluation of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil

Cited by 16 publications

References 15 publications

Surveillance and response: Tools and approaches for the elimination stage of neglected tropical diseases

Surveillance and response: Tools and approaches for the elimination stage of neglected tropical diseases

Detection of low‐intensity Schistosoma mansoni infection by Percoll sedimentation and real‐time PCR techniques in a low‐endemicity Egyptian setting

Nucleic acid detection in the diagnosis and prevention of schistosomiasis

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