Comparison of the Kato-Katz and Helmintex methods for the diagnosis of schistosomiasis in a low-intensity transmission focus in Bandeirantes, Paraná, southern Brazil

Caldeira, Kerlen; Teixeira, Candida Fagundes; Silveira, Milene Selbach; Fries, Lucas Castello Costa de; Romanzini, Juliano; Bittencourt, Hélio Radke; Graeff-Teixeira, Carlos

doi:10.1590/s0074-02762012000500019

Cited by 20 publications

(19 citation statements)

References 12 publications

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“…More recently, after the WHO's appeal to define the value of the rapid urine test (POC-CCA) for schistosomiasis in lowendemic areas, Brazilian investigators have published papers on the subject [37][38][39] . Preliminary information is being evaluated, and other studies in Brazil are under way.…”

Section: Discussionmentioning

confidence: 99%

Sensitivity and specificity of the circulating cathodic antigen rapid urine test in the diagnosis of Schistosomiasis mansoni infection and evaluation of morbidity in a low- endemic area in Brazil

Ferreira

Fidelis

Pereira

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Section: Discussionmentioning

confidence: 99%

Sensitivity and specificity of the circulating cathodic antigen rapid urine test in the diagnosis of Schistosomiasis mansoni infection and evaluation of morbidity in a low- endemic area in Brazil

Ferreira

Fidelis

Pereira

et al. 2017

Rev. Soc. Bras. Med. Trop.

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“…This investigation was conducted to elucidate the processes underlying the success of a previously developed magnetic fractionation approach for the detection of parasite eggs in fecal samples, namely the Helmintex method [11]. In recent years, this method has been used in a series of diagnostic studies and has consistently shown improved sensitivity when compared with the conventional Kato-Katz method of fecal evaluation and the saline gradient method [11], [25], [26], [27].…”

Section: Discussionmentioning

confidence: 99%

“…Systematic quantification of the binding of microspheres that have different surface functionalizations to parasite eggs is likely to represent an opportunity to optimize the Helmintex magnetic fractionation method. Previous field studies have indicated that such an optimized Helmintex method may be developed into a new gold standard to validate future rapid diagnostic and molecular methods for Schistosoma detection [25], [26], [27].…”

Section: Discussionmentioning

confidence: 99%

The Iron Distribution and Magnetic Properties of Schistosome Eggshells: Implications for Improved Diagnostics

et al. 2013

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Background Schistosoma mansoni and Schistosoma japonicum are the most frequent causative agents of human intestinal schistosomiasis. Approximately 200 million people in the world are infected with schistosomes. Diagnosis of schistosomiasis is often difficult. High percentages of low level infections are missed in routine fecal smear analysis and current diagnostic methodologies are inadequate to monitor the progress of parasite control, especially in areas with low transmission. Improved diagnostic methods are urgently needed to evaluate the success of elimination programs. Recently, a magnetic fractionation method for isolation of parasite eggs from feces was described, which uses magnetic microspheres to form parasite egg – magnetic microsphere conjugates. This approach enables screening of larger sample volumes and thus increased diagnostic sensitivity. The mechanism of formation of the conjugates remains unexplained and may either be related to specific surface characteristics of eggs and microspheres or to their magnetic properties.Methods/Principal FindingsHere, we investigated iron localization in parasite eggs, specifically in the eggshells. We determined the magnetic properties of the eggs, studied the motion of eggs and egg-microsphere conjugates in magnetic fields and determined species specific affinity of parasite eggs to magnetic microspheres. Our study shows that iron is predominantly localized in pores in the eggshell. Parasite eggs showed distinct paramagnetic behaviour but they did not move in a magnetic field. Magnetic microspheres spontaneously bound to parasite eggs without the presence of a magnetic field. S. japonicum eggs had a significantly higher affinity to bind microspheres than S. mansoni eggs.Conclusions/SignificanceOur results suggest that the interaction of magnetic microspheres and parasite eggs is unlikely to be magnetic in origin. Instead, the filamentous surface of the eggshells may be important in facilitating the binding. Modification of microsphere surface properties may therefore be a way to optimize magnetic fractionation of parasite eggs.

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“…In addition, Schistosomiasis cases have non-specific increase in white blood cells with counts usually ranging up to 10-30 x10 9 /L and with eosinophil ratio between 0.15 and 0.20 [10]. However, some clinical reports indicate that schistosomiasis patients show no changes in eosinophils count [11]. Therefore, eosinophil counts can only assist in the diagnosis of acute schistosomiasis.…”

Section: Routine Examinationmentioning

confidence: 99%

Advances in diagnosis of schistosomiasis.

Gu¹,

Wm²,

Zhisheng³

et al. 2018

Microbiology

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Schistosomiasis is a kind of globally infectious tropical disease that endangers human health and hinders social development in modern society. The main epidemic species is Schistosoma japonicum in China. Occasionally cases of imported schistosomiasis are reported. This article provides new insights to the research of clinical diagnosis and treatment by summarizing the common diagnosis methods of schistosomiasis in China, such as routine examination, pathogen detection, immunological tests, nucleic acid tests and medical imaging examination.

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Comparison of the Kato-Katz and Helmintex methods for the diagnosis of schistosomiasis in a low-intensity transmission focus in Bandeirantes, Paraná, southern Brazil

Cited by 20 publications

References 12 publications

Sensitivity and specificity of the circulating cathodic antigen rapid urine test in the diagnosis of Schistosomiasis mansoni infection and evaluation of morbidity in a low- endemic area in Brazil

Sensitivity and specificity of the circulating cathodic antigen rapid urine test in the diagnosis of Schistosomiasis mansoni infection and evaluation of morbidity in a low- endemic area in Brazil

The Iron Distribution and Magnetic Properties of Schistosome Eggshells: Implications for Improved Diagnostics

Advances in diagnosis of schistosomiasis.

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