A combined approach of VNTR and MLST analysis: improving molecular typing of Argentinean isolates of Leptospira interrogans

Caimi, Karina; Varni, Vanina Delia; Melendez, Yamil; Koval, Ariel; Brihuega, Bibiana; Ruybal, Paula

doi:10.1590/s0074-02762012000500011

Cited by 25 publications

(22 citation statements)

References 30 publications

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“…MLVA showed a strong correlation with the secY sequence analysis, which was previously used for taxonomic and epidemiological purposes (Ahmed et al, 2006;Perez & Goarant, 2010;Paiva-Cardoso et al, 2013). Amongst the molecular techniques, genotyping L. interrogans strains by MLVA is increasingly being utilized for identification and epidemiological studies (Slack et al, 2005;Li et al, 2012;Caimi et al, 2012). In the present study, MLVA of L. santarosai showed that serovars from either the same serogroup or the same geographical area were not grouped together.…”

Section: Discussionsupporting

confidence: 58%

“…Discrepancies between serology and genetic classification are not unexpected. Several authors have commented on this, and there is a consensus that more isolates and loci are needed to obtain an improved typing method for Leptospira characterization (Nalam et al, 2010;Caimi et al, 2012). Thus, a combined molecular (VNTR, multilocus sequence typing) and serological analysis seems to be a better approach for the typing of Leptospira isolates in order to obtain a greater insight into the evolutionary biology and epidemiology of this important pathogen.…”

Section: Discussionmentioning

confidence: 99%

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A multilocus variable number tandem repeat analysis assay provides high discrimination for genotyping Leptospira santarosai strains

Hamond

Pinna

Medeiros

et al. 2015

Journal of Medical Microbiology

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Considering the prevalence of Leptospira santarosai infections in the Americas and the scarce information about the species, we aimed to apply a multilocus variable number tandem repeat (VNTR) analysis (MLVA) for the molecular typing of L. santarosai isolates from various sources. Amplification of three VNTR loci selected from L. santarosai genome sequences resulted in a wide range of sizes for the amplified products amongst the 21 L. santarosai strains analysed. This suggested a variation in tandem repeat copy numbers in the VNTR loci. secY sequencing also showed a high nucleotide diversity, confirming the MLVA data. In conclusion, this novel MLVA provided a high level of discrimination between L. santarosai isolates, and this new typing tool could be used to investigate leptospirosis in regions where L. santarosai predominates.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

A multilocus variable number tandem repeat analysis assay provides high discrimination for genotyping Leptospira santarosai strains

Hamond

Pinna

Medeiros

et al. 2015

Journal of Medical Microbiology

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“…Nowadays, multilocus sequence typing (MLST) method has been recently proved for typing leptospires [4,13-15]. MLST is a simple PCR based technique, which makes use of automated DNA sequencers to assign and characterize the alleles present in different target genes.…”

Section: Introductionmentioning

confidence: 99%

Source tracking of human leptospirosis: serotyping and genotyping of Leptospira isolated from rodents in the epidemic area of Guizhou province, China

Wang

Zhang

et al. 2013

BMC Microbiology

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BackgroundSustained human leptospirosis as well as death cases has been reported in Qiandongnan Prefecture, Southeast of Guizhou, China, recently, but these human patients were only clinically diagnosed, and leptospires have never been isolated from patients in these epidemic regions, In order to track the source of infection and understand the etiologic characteristic of leptospirosis, we performed rodent carrier surveillance for leptospirosis in the epidemic area in 2011. The population distribution of rodents in the epidemic regions was revealed.ResultsFour strains of leptospire were isolated from Apodemus agrarius. Microscopic agglutination test (MAT) confirmed the four isolates belonged to leptospiral serogroup Icterohaemorrhagiae. Multilocus sequence typing (MLST) indicated that all the four strains were defined as sequence type 1(ST1), which is identical to the three strains isolated from Rattus tanezumi in Rongjiang County in 2007. Clustering analysis of the MLST data indicated that the local isolates exactly matched with reference strain of leptospiral serovar Lai strain 56601, which is consistent with anti-Leptospira antibody detection of patients using MAT.ConclusionsApodemus agrarius may be the potentially important carrier of leptospirosis and the potential source of leptospiral infection in human, and serovar Lai maybe the epidemic serovar of Leptospira in the localities.

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“…Noteworthy, ST (7LBoonsilp) 17 isolates seem to be widespread and were found to be also associated with human infections in Japan, Brazil, Belgium and Denmark (Boonsilp et al, ). This sequence type seems to be responsible for up to 90% of human leptospirosis cases in São Paulo, Brazil (Romero, Blanco, & Galloway, ) and was also identified in a rat, pig and human hosts in Argentina (Caimi et al, ). It seems to be also frequent in Russia (Voronina et al, ) and China (Zhang et al, ).…”

Section: Discussionmentioning

confidence: 96%

Genetic diversity of pathogenic leptospires from wild, domestic and captive host species in Portugal

Ferreira

Ahmed

Rocha

et al. 2019

Transbound Emerg Dis

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Leptospirosis is a neglected zoonotic disease of worldwide distribution with a significant veterinary and public health impact. It is caused by pathogenic bacteria of the genus Leptospira. The availability of effective tools to accurately identify and type leptospires is of utmost importance for the diagnosis of the disease and for assessing its epidemiology. Several multi‐locus sequence typing (MLST) approaches were described for the typing of worldwide isolates of Leptospira but an extensive agreement towards the adoption of a unique consensus scheme for this agent is still lacking. Most genotyped strains originate from Asian and South American countries, with a minority originating from Europe (being most countries represented only by one or a few isolates). The knowledge of the diversity of circulating leptospires is the key to understanding the disease transmission and its zoonotic implications. In this study, we revisited the taxonomy of several isolates of pathogenic Leptospira obtained from domestic, wild and captive animals in Portugal, between 1990 and 2012. A selection of these isolates was genotyped using two previously published MLST schemes. A total of seven distinct sequence types (STs) were detected among the Portuguese isolates with two STs representing L. borgpetersenii (ST149 and ST152), two STs representing L. kirschneri (ST117 and ST100) and three STs representing L. interrogans (ST17, ST24 and ST140). Global widespread (and maybe more virulent) Leptospira genotypes seem to circulate in Portugal, particularly the L. interrogans ST17 isolates which are associated with several outbreaks of leptospirosis among humans and animals in different regions of the world. This study contributes to the enrichment of the global MLST databases with a new set of allele and sequence type information also providing novel data on circulating Leptospira serovars in Portugal.

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A combined approach of VNTR and MLST analysis: improving molecular typing of Argentinean isolates of Leptospira interrogans

Cited by 25 publications

References 30 publications

A multilocus variable number tandem repeat analysis assay provides high discrimination for genotyping Leptospira santarosai strains

A multilocus variable number tandem repeat analysis assay provides high discrimination for genotyping Leptospira santarosai strains

Source tracking of human leptospirosis: serotyping and genotyping of Leptospira isolated from rodents in the epidemic area of Guizhou province, China

Genetic diversity of pathogenic leptospires from wild, domestic and captive host species in Portugal

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