Validation of a modified fluorimetric assay for the screening of trichomonacidal drugs

Escribano, Alicia; Marcel, Alfredo Meneses; Tugores, Yanetsy Machado; Nogal-Ruiz, Juan José; Redó, Vicente J Arán; García-Trevijano, José Antonio Escario; Gómez‐Barrio, Alicia

doi:10.1590/s0074-02762012000500010

Cited by 17 publications

(26 citation statements)

References 35 publications

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“…Studies of activity against T. vaginalis were carried out as reported previously . Initial activity assays were performed on trophozoites of the metronidazole‐sensitive JH31A#4 isolate, and the activity values are included in Table .…”

Section: Resultsmentioning

confidence: 99%

Antichagasic, Leishmanicidal, and Trichomonacidal Activity of 2‐Benzyl‐5‐nitroindazole‐Derived Amines

et al. 2018

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Three different series of new 5-nitroindazole derivatives-1-(ω-aminoalkyl)-2-benzylindazolin-3-ones (series A; ten compounds), 3-(ω-aminoalkoxy)-2-benzylindazoles (series B; four compounds) and 3-alkylamino-2-benzylindazoles (series C; five compounds)-have been synthesized and evaluated against the protozoan parasites Trypanosoma cruzi, Leishmania amazonensis, and Trichomonas vaginalis: etiological agents of Chagas disease, cutaneous leishmaniasis, and trichomoniasis, respectively. Many indazoles of series A, B, and C were efficient against T. cruzi. Some compounds in series A, after successfully passing the preliminary screening for epimastigotes, exhibited activity values against amastigotes of several T. cruzi strains that were better than or similar to those shown by the reference drug benznidazole and displayed low nonspecific toxicity against mammalian cells. On the other hand, preliminary studies against promastigotes of L. amazonensis showed high leishmanicidal activity for some derivatives of series A and C. With regard to activity against T. vaginalis, some indazoles of series B and C were rather efficient against trophozoites of a metronidazole-sensitive isolate and showed low nonspecific toxicities toward Vero cell cultures. Additionally, some of these compounds displayed similar activity against metronidazole-sensitive and resistant isolates, showing the absence of cross-resistance between these derivatives and the reference drug.

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Section: Resultsmentioning

confidence: 99%

Antichagasic, Leishmanicidal, and Trichomonacidal Activity of 2‐Benzyl‐5‐nitroindazole‐Derived Amines

et al. 2018

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“…vaginalis isolates C-1:NIH and IR 78 were obtained from the American Type Culture Collection and grown in TYM medium supplemented with 10% heat-inactivated fetal bovine serum and antibiotics (Ibáñez Escribano et al, 2012). Trichomonads were batch cultured in glass tubes at 37 • C and passaged every 48-72 h.…”

Section: T Vaginalis Organismsmentioning

confidence: 99%

“…This redox dye is reduced to a fluorescent compound by reductases presented only in viable parasites. The determination of sensitivity to human sera is as described recently (Ibáñez Escribano et al, 2012). For all conditions, two independent experiments were performed using eight replicate samples.…”

Section: Lytic Assay To Determine Trichomonad Susceptibility To Complmentioning

confidence: 99%

“…For all conditions, two independent experiments were performed using eight replicate samples. A standard curve was obtained as described before (Ibáñez Escribano et al, 2012) where a linear correlation was obtained. This fluorimetric assay permits excitation at the wavelength of 535 nm and an emission wavelength of 590 nm, which measures live organisms.…”

Section: Lytic Assay To Determine Trichomonad Susceptibility To Complmentioning

confidence: 99%

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Sequestration of host-CD59 as potential immune evasion strategy of Trichomonas vaginalis

et al. 2015

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“…Alamar Blue® and its major component, resazurin, are blue and non-fluorescent dyes that become pink and fluorescent (resorufin) by the activity of oxidoreductases present in viable cells (Escribano et al, 2012). However, attempts to validate this method for Trichomonas vaginalis susceptibility by Natto et al indicated that this assay poses several inherent challenges because of the reduction activity of…”

Section: Two-step-assay Descriptionmentioning

confidence: 99%

Challenges to get axenic cultures of Trichomonas spp. — A new approach in eradication of contaminants and maintenance of laboratory microbiological cultures

Koptina

Strese

Backlund

et al. 2015

Journal of Microbiological Methods

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Validation of a modified fluorimetric assay for the screening of trichomonacidal drugs

Cited by 17 publications

References 35 publications

Antichagasic, Leishmanicidal, and Trichomonacidal Activity of 2‐Benzyl‐5‐nitroindazole‐Derived Amines

Antichagasic, Leishmanicidal, and Trichomonacidal Activity of 2‐Benzyl‐5‐nitroindazole‐Derived Amines

Sequestration of host-CD59 as potential immune evasion strategy of Trichomonas vaginalis

Challenges to get axenic cultures of Trichomonas spp. — A new approach in eradication of contaminants and maintenance of laboratory microbiological cultures

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