The activity of echinocandins, amphotericin B and voriconazole against fluconazole-susceptible and fluconazole-resistant Brazilian Candida glabrata isolates

Mario, Débora Alves Nunes; Denardi, Laura Bedin; Bandeira, Laíssa Arévalo; Antunes, Milene Silva; Santúrio, Jânio Morais; Severo, Luiz Carlos; Alves, Sydney Hartz

doi:10.1590/s0074-02762012000300022

Cited by 20 publications

(18 citation statements)

References 20 publications

(16 reference statements)

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“…Amphotericin B is generally applied for primary therapy of severe infections caused by C. glabrata [101] but high MIC values of 25 µg/mL against some strains have been demonstrated in agreement with our results [102,103]. Moreover, 5 % of nosocomial isolates of C. glabrata have been found to be resistant to caspofungin, a drug belonging to the newest class of antifungals, the echinocandins, which target cell wall synthesis [44].…”

Section: Accepted Manuscriptsupporting

confidence: 88%

Epidihydropinidine, the main piperidine alkaloid compound of Norway spruce (Picea abies) shows promising antibacterial and anti-Candida activity

et al. 2017

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ArtikkelitLuonnontieteiden This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T

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Section: Accepted Manuscriptsupporting

confidence: 88%

Epidihydropinidine, the main piperidine alkaloid compound of Norway spruce (Picea abies) shows promising antibacterial and anti-Candida activity

et al. 2017

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“…However, recent epidemiological surveys have revealed the emergence of increasing numbers of isolates that have decreased susceptibility to echinocandins, particularly among fluconazole resistant isolates [10–12]. Furthermore, it has also been reported that polyene susceptibility is attenuated in azole-resistant isolates [11]. …”

Section: Introductionmentioning

confidence: 99%

Unexpected effects of azole transporter inhibitors on antifungal susceptibility in Candida glabrata and other pathogenic Candida species

et al. 2017

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The pathogenic fungus Candida glabrata is often resistant to azole antifungal agents. Drug efflux through azole transporters, such as Cdr1 and Cdr2, is a key mechanism of azole resistance and these genes are under the control of the transcription factor Pdr1. Recently, the monoamine oxidase A (MAO-A) inhibitor clorgyline was shown to inhibit the azole efflux pumps, leading to increased azole susceptibility in C. glabrata. In the present study, we have evaluated the effects of clorgyline on susceptibility of C. glabrata to not only azoles, but also to micafungin and amphotericin B, using wild-type and several mutant strains. The addition of clorgyline to the culture media increased fluconazole susceptibility of a C. glabrata wild-type strain, whereas micafungin and amphotericin B susceptibilities were markedly decreased. These phenomena were also observed in other medically important Candida species, including Candida albicans, Candida parapsilosis, Candida tropicalis, and Candida krusei. Expression levels of CDR1, CDR2 and PDR1 mRNAs and an amount of Cdr1 protein in the C. glabrata wild-type strain were highly increased in response to the treatment with clorgyline. However, loss of Cdr1, Cdr2, Pdr1, and a putative clorgyline target (Fms1), which is an ortholog of human MAO-A, or overexpression of CDR1 did not affect the decreased susceptibility to micafungin and amphotericin B in the presence of clorgyline. The presence of other azole efflux pump inhibitors including milbemycin A4 oxime and carbonyl cyanide 3-chlorophenylhydrazone also decreased micafungin susceptibility in C. glabrata wild-type, Δcdr1, Δcdr2, and Δpdr1 strains. These findings suggest that azole efflux pump inhibitors increase azole susceptibility but concurrently induce decreased susceptibility to other classes of antifungals independent of azole transporter functions.

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“…This process will promote selection of innately resistant strains or development of secondary resistance to these drugs in a previously susceptible C.albicans [8,9].…”

Section: Introductionmentioning

confidence: 99%

Efficacies of Echinocandins versus Azoles Antifungal agents in Management of Candidaalbicans andCandida dubliniensis

Mohtady¹,

Meawed²,

Nassar³

et al. 2015

iajaa

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Background: Candida infection is a world wide problem with greatly changed epidemiology. Candida dubliniensis is an emerging non-albicans, shares several phenotypic and genotypic characteristics and closely related to Candida albicans. Fluconazole-resistant Candida spp. becomes a significant life-threatening problem especially in high risk patients. Echinocandins are promising antifungals with no recorded resistance. This study was done, first to differentiate C. dubliniensis from C. albicans by different phenotypic and molecular methods and to compare in vitro activities of echinocandins versus azoles against Candida spp.

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The activity of echinocandins, amphotericin B and voriconazole against fluconazole-susceptible and fluconazole-resistant Brazilian Candida glabrata isolates

Cited by 20 publications

References 20 publications

Epidihydropinidine, the main piperidine alkaloid compound of Norway spruce (Picea abies) shows promising antibacterial and anti-Candida activity

Epidihydropinidine, the main piperidine alkaloid compound of Norway spruce (Picea abies) shows promising antibacterial and anti-Candida activity

Unexpected effects of azole transporter inhibitors on antifungal susceptibility in Candida glabrata and other pathogenic Candida species

Efficacies of Echinocandins versus Azoles Antifungal agents in Management of Candidaalbicans andCandida dubliniensis

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