Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)

Segura, Nidya Alexandra; Santamaría, Erika; Cabrera, Olga Lucía; Bello, Felio J.

doi:10.1590/s0074-02762012000100013

Cited by 8 publications

(4 citation statements)

References 28 publications

(35 reference statements)

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“…GRIP-1Embryos[23]A.t. GRIP-2 & -3Larvae Aedes vexans West Nile virusUM-AVE1Embryos[26]Rift Valley fever virus Anopheles gambiae MalariaMos.55Larvae[27]Sua1BLarvae[28]4a-3BLarvae Anopheles stephensi MalariaMos.43Larvae[29]MSQ43Larvae[30] Anopheles albimanus MalariaLSB-AA695BBEmbryos[31] Culex quinquefasciatus West Nile virusUnnamedOvaries[32]Lymphatic filariasisUnnamedEmbryos[33] Culex theileri Rift Valley fever virusUnnamedEmbryos[34]Dog heartworm Culex tritaeniorhynchus Japanese encephalitis virusNIID-CTREmbryos[36] Culex bitaeniorhynchus Japanese encephalitis virusUnnamedEmbryos[37] Toxorhynchites amboinensis -TRA-171Larvae[38]…”

Section: Reviewmentioning

confidence: 99%

“…A cell line was established from this species using ovaries as the source tissue in the 1960s [32], and more recently a Cx. quinquefasciatus cell line has been established from embryos [33]. Cx.…”

Section: Reviewmentioning

confidence: 99%

“…An important consideration in the use of embryos for cell culture is the timing of embryogenesis. An incubation period after egg laying of 16-20 hours (approximately 66% of the time prior to larval hatching) was shown to be optimal for the successful generation of mosquito cell lines [31, 33]. A change in temperature during incubation has also been shown to facilitate establishment of primary cell cultures, possibly through stimulation of cell division [34].…”

Section: Reviewmentioning

confidence: 99%

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Mosquito cell lines: history, isolation, availability and application to assess the threat of arboviral transmission in the United Kingdom

et al. 2014

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Mosquitoes are highly effective vectors for transmission of human and animal pathogens. Understanding the relationship between pathogen and vector is vital in developing strategies to predict and prevent transmission. Cell lines derived from appropriate mosquito hosts provide a relatively simple tool for investigating the interaction between the host and viruses transmitted by mosquitoes. This review provides a brief overview of the development of mosquito cell lines, methods of isolation, their availability and application for investigating insect-virus interactions.

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Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

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Mosquito cell lines: history, isolation, availability and application to assess the threat of arboviral transmission in the United Kingdom

et al. 2014

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“…Analysing such organisms' life-cycle stages and their colonisation under laboratory conditions might provide access to valuable biological material for undertaking basic and applied research in medicine and forensic sciences. Additional studies of colonised insects have been related to systematics, bionomy, genetics, insecticide susceptibility, vector competence, vector capacity, vaccine development and the establishment of cell cultures [9,10].…”

mentioning

confidence: 99%

Sarconesiopsis magellanica (Diptera: Calliphoridae) life-cycle, reproductive and population parameters using different diets under laboratory conditions

Pinilla

Patarroyo

Bello

2013

Forensic Science International

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Establishment and characterization of two new cell lines from the mosquito Armigeres subalbatus (Coquillett) (Diptera: Culicidae)

Hoshino

Isawa

Kuwata

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

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Armigeres subalbatus (Coquillett) is a medically important mosquito and a model species for immunology research. We successfully established two cell lines from the neonate larvae of A. subalbatus using two different media. To our knowledge, this is the first report of an established Armigeres mosquito cell line. The cell lines, designated as Ar-3 and Ar-13, consist of adherent and diploid cells with compact colonies. Both these cell lines grow slowly after passage at a split ratio of 1:5 and a population doubling time of 2.7 and 3.0 d, respectively. Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to confirm that these lines correspond to the species of origin and are clearly distinct from seven other insect cell lines. Furthermore, reverse-transcription PCR was used to demonstrate that the Ar-3 cell line is susceptible to the Japanese encephalitis virus and two insect flaviviruses associated with Culex and Aedes mosquitoes but relatively insensitive to dengue virus. These data indicate that the newly established cell lines are cellular models of A. subalbatus as well as beneficial tools for the propagation of viruses associated with the Armigeres mosquito.

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Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)

Cited by 8 publications

References 28 publications

Mosquito cell lines: history, isolation, availability and application to assess the threat of arboviral transmission in the United Kingdom

Mosquito cell lines: history, isolation, availability and application to assess the threat of arboviral transmission in the United Kingdom

Sarconesiopsis magellanica (Diptera: Calliphoridae) life-cycle, reproductive and population parameters using different diets under laboratory conditions

Establishment and characterization of two new cell lines from the mosquito Armigeres subalbatus (Coquillett) (Diptera: Culicidae)

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