Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates

Ribeiro, Ricardo Souza; Ladeira, Luisa; Rezende, Antônio Mauro; Fontes, Cor Jésus Fernandes; Carvalho, Luzia H.; Brito, Cristiana Ferreira Alves de

doi:10.1590/s0074-02762011000900004

Cited by 10 publications

(9 citation statements)

References 34 publications

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“…However, outside Anhui province, the allele PH3, the second most common one in the present study, was the most prevalent in other areas of China including Hainan and Yunnan provinces, and also in Myanmar [7]. All of the Hha I allelic types found in the present study have been reported in other regions of the world, including Myanmar (PH1, PH3, PH4, PH5, and PH6) [7], India Chennai (PH1, PH4, PH5, and PH6) [11,21], Colombia (PH1, PH2, PH3, PH4, PH5, and PH6) [22], Brazil (PH3 and PH4) [26], and Thailand (PH1, PH3, PH4, PH6, and PH7) [28]. In China, five of the seven alleles were reported previously, e.g., PH1, PH3, PH4, PH5, and PH6 from Suzhou city of Anhui province; PH3, PH4, PH5, and PH6 from Hainan province; and PH1, PH3, and PH4 from Yunnan province [7].…”

Section: Discussionsupporting

confidence: 56%

Genetic diversity of Plasmodium vivax population in Anhui province of China

Huang

et al. 2014

Malar J

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BackgroundAlthough the numbers of malaria cases in China have been declining in recent years, outbreaks of Plasmodium vivax malaria were still being reported in rural areas south of the Yellow River. To better understand the transmission dynamics of P. vivax parasites in China, the extent of genetic diversity of P. vivax populations circulating in Bozhou of Anhui province of China were investigated using three polymorphic genetic markers: merozoite surface proteins 1 and 3α (pvmsp-1 and pvmsp-3α) and circumsporozoite protein (pvcsp).MethodsForty-five P. vivax clinical isolates from Bouzhou of Anhui province were collected from 2009 to 2010 and were analysed using PCR/RFLP or DNA sequencing.ResultsSeven and six distinct allelic variants were identified using PCR/RFLP analysis of pvmsp-3α with HhaI and AluI, respectively. DNA sequence analysis of pvmsp-1 (variable block 5) revealed that there were Sal-I and recombinant types but not Belem type, and seven distinct allelic variants in pvmsp-1 were detected, with recombinant subtype 2 (R2) being predominant (66.7%). All the isolates carried pvcsp with VK210 type but not VK247 or P. vivax-like types in the samples. Sequence analysis of pvcsp gene revealed 12 distinct allelic variants, with VK210-1 being predominant (41.5%).ConclusionsThe present data indicate that there is some degree of genetic diversity among P. vivax populations in Anhui province of China. The genetic data obtained may assist in the surveillance of P. vivax infection in endemic areas or in tracking potential future disease outbreak.

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Section: Discussionsupporting

confidence: 56%

Genetic diversity of Plasmodium vivax population in Anhui province of China

Huang

et al. 2014

Malar J

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“…The groups of sequences sharing similarly sized amplicons were termed size classes, and the number of sequences within each varied from 27 in class 1 to just a single isolate (Indonesia-INDO94) in class 5 (Table 3). The expected length of the internal region amplified by nested PCR ranged from 1137 to 1950 bp, consistent with what has been seen previously in locations such as Sri Lanka [32] and Brazil [30]. Alleles with an even larger deletion in the central region of the gene, and thus a smaller amplicon, have been observed in India [26] and Pakistan [24], but none were recovered here.…”

Section: Resultssupporting

confidence: 89%

“…In such a case, rather than deletions being responsible for the shorter alleles seen in P. vivax , large insertions in a shorter ancestral sequence would explain the range in allele length. The direction of indel mutations cannot be inferred from alignment, however, and the larger P. vivax alleles have been found repeatedly to be the predominant type (e.g., [13,26,30,31,33], as was the case in this sample here. Further, diversity among indel haplotypes for sequences with lengths over 2500 bp (indel haplotypes 3-6) varied, but was within the range seen for all haplotype groups in P. vivax (Table 2).…”

Section: Resultsmentioning

confidence: 48%

Merozoite surface protein-3 alpha as a genetic marker for epidemiologic studies in Plasmodium vivax: a cautionary note

Rice

Acosta

Pacheco

et al. 2013

Malar J

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BackgroundPlasmodium vivax is the most widespread of the human malaria parasites in terms of geography, and is thought to present unique challenges to local efforts aimed at control and elimination. Parasite molecular markers can provide much needed data on P. vivax populations, but few such markers have been critically evaluated. One marker that has seen extensive use is the gene encoding merozoite surface protein 3-alpha (MSP-3α), a blood-stage antigen known to be highly variable among P. vivax isolates. Here, a sample of complete msp-3α gene sequences is analysed in order to assess its utility as a molecular marker for epidemiologic investigations.MethodsAmplification, cloning and sequencing of additional P. vivax isolates from different geographic locations, including a set of Venezuelan field isolates (n = 10), yielded a sample of 48 complete msp-3α coding sequences. Characterization of standard population genetic measures of diversity, phylogenetic analysis, and tests for recombination were performed. This allowed comparisons to patterns inferred from the in silico simulation of a polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) protocol used widely.ResultsThe larger sample of MSP-3α diversity revealed incongruence between the observed levels of nucleotide polymorphism, which were high in all populations, and the pattern of PCR-RFLP haplotype diversity. Indeed, PCR-RFLP haplotypes were not informative of a population’s genetic diversity and identical haplotypes could be produced from analogous bands in the commonly used protocol. Evidence of frequent and variable insertion-deletion mutations and recurrent recombination between MSP-3α haplotypes complicated the inference of genetic diversity patterns and reduced the phylogenetic signal.ConclusionsThe genetic diversity of P. vivax msp-3α involves intragenic recombination events. Whereas the high genetic diversity of msp-3α makes it a promising marker for some epidemiological applications, the ability of msp-3α PCR-RFLP analysis to accurately track parasites is limited. Local studies of the circulating alleles are needed before implementing PCR-RFLP approaches. Furthermore, evidence from the global sample analysed here suggests such msp-3α PCR-RFLP methods are not suitable for broad geographic studies or tracking parasite populations for an extended period of time.

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“…The present study demonstrates that the level of genetic diversity of the P.vivax population in Sri Lanka is remarkably lower than the levels in other tropical and subtropical areas reported by Prajapati et al, Adhikari et al, and Ribeiro et al, (Prajapati et al, 2010;Adhikari et al, 2012;Ribeiro et al, 2011). A study done in Chennai, India in 2010 showed 14 distinct alleles among 22 isolates (Prajapati et al, 2010).…”

Section: Polymorphism At the Merozoite Surface Protein-3α Locus Of Plmentioning

confidence: 44%

“…A study done in Chennai, India in 2010 showed 14 distinct alleles among 22 isolates (Prajapati et al, 2010). The studies done in Jhapa district in Nepal and in Brazil showed 13 in 56 isolates and 11 distinct alleles in 52 isolates respectively (Adhikari et al, 2012, Ribeiro et al, 2011. A dramatic reduction of P. vivax case load has been observed in Sri Lanka over the period 2000-2011.…”

Section: Polymorphism At the Merozoite Surface Protein-3α Locus Of Plmentioning

confidence: 99%

Polymorphism at the Merozoite Surface Protein-3α Locus of Plasmodium vivax in Sri Lanka

Wahalathanthri¹

2014

J. adv. parasitol.

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Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates

Cited by 10 publications

References 34 publications

Genetic diversity of Plasmodium vivax population in Anhui province of China

Genetic diversity of Plasmodium vivax population in Anhui province of China

Merozoite surface protein-3 alpha as a genetic marker for epidemiologic studies in Plasmodium vivax: a cautionary note

Polymorphism at the Merozoite Surface Protein-3α Locus of Plasmodium vivax in Sri Lanka

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