Characterisation of virulence genes in methicillin susceptible and resistant Staphylococcus aureus isolates from a paediatric population in a university hospital of Medellín, Colombia

Jiménez, J. Natalia; Ocampo, Ana M.; Vanegas, Johanna M.; Rodríguez, Erika A.; Garcés, Carlos; Patiño, Luz Adriana; Ospina, Sigifredo; Correa, Margarita M.

doi:10.1590/s0074-02762011000800013

Cited by 53 publications

(52 citation statements)

References 38 publications

(40 reference statements)

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“…In the present study, the incidence rate of the eta gene was higher than that reported in other studies from Iran 0.68% (12), Colombia (3%) (29), and Malaysia (0%) (6). Unlike the results of the present study, high prevalence of the eta gene was reported in studies conducted in Czech (10%) (5) and Turkey 19.2% (32).…”

Section: Discussioncontrasting

confidence: 91%

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Distribution of Adhesion and Toxin Genes in Staphylococcus aureus Strains Recovered From Hospitalized Patients Admitted to the ICU

Eftekhar

Rezaee

Azad

et al. 2016

Arch Pediatr Infect Dis

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) strains are a common cause of emerging nosocomial infections and are a major public health concern. Objectives: The aim of the present study was to determine the prevalence of MRSA, its antibiotic resistance pattern, and the virulence gene profiles in hospitalized patients admitted to ICUs. Methods: During a 6-month period, a total of 70 S. aureus isolates were collected from 249 patients admitted to the ICU in five hospitals. In vitro antibiotic susceptibility testing of the S. aureus isolates was carried out using the Kirby-Bauer disk diffusion method with 16 antibiotic disks. Molecular detection of toxin and adhesion genes was carried out using PCR. Results: All the 70 S. aureus isolates were confirmed to be MRSA strains. The largest number of S. aureus isolates was found in the blood (42.9%) and wound (21.4%) samples. The MDR pattern was detected in 71.4% of the isolates, which were obtained from wound and blood samples. Simultaneous resistance to seven, six, five, four and three drugs was common in 35 (50%), 7 (10%), 8 (11.4%), 11 (15.7%), 2 (2.9%) and 5 (7.1%) isolates, respectively. The frequency of the spa, fnbB, fnbA, clfB, clfA, can, bbp, ebp, etb, eta, pvl, and tst genes was 100%, 75.7%, 74.3%, 78.6%, 71.4%24.3%, 0%, 58.6%, 2.9%, 7.1%, 21.4%, and 51.4%, respectively. In addition, among all the examined genes, the clfB (78.6%) and etb (2.9%) genes had the highest and lowest prevalence respectively. Conclusions: In the present study, we found a high prevalence of MRSA at the hospitals studied. The findings emphasized the increased prevalence of MRSA isolates containing different toxin and adhesion genes, probably accompanied by antimicrobial resistance. Infection with such isolates worsens the clinical outcomes as well as the morbidity and mortality rates in hospitalized patients in ICUs.

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Section: Discussioncontrasting

confidence: 91%

“…The most prevalent toxin gene was found to be tst, with a prevalence rate of 51.4%, which is higher than that reported in previous studies conducted in Iran (11.6%) (12), Sweden (22%) (28), Malaysia (0.5%) (6) and Colombia (10%) (29). An interesting finding in the present study is that Arch Pediatr Infect Dis.…”

Section: Discussioncontrasting

confidence: 65%

Distribution of Adhesion and Toxin Genes in Staphylococcus aureus Strains Recovered From Hospitalized Patients Admitted to the ICU

Eftekhar

Rezaee

Azad

et al. 2016

Arch Pediatr Infect Dis

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“…The results obtained in the current study are higher than those reported in Sweden 22% (37) and Colombia (10%) (38) and lower than a study conducted in Iran 51.4% (1). In line with a study which reported the prevalence of 2% -35% of PVL genes among MRSA strains (39), 25 isolates (26.3%) obtained from blood (37%) and wound (33.3%) which belonged to SCCmec type IV were positive for the PVL-encoding genes.…”

Section: Discussioncontrasting

confidence: 90%

“…The frequency of the eta gene in this study was 1.1%. The frequency rates of eta gene reported from Colombia (38) and Malaysia (40) were 3% and 0%, respectively. The range from 0% in Colombia (38) and Malaysia (40) to 9.2% in Turkey (41) for the etb gene frequency was reported.…”

Section: Discussionmentioning

confidence: 94%

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Molecular Characterization and Resistance Profile of Methicillin Resistant Staphylococcus aureus Strains Isolated from Hospitalized Patients in Intensive Care Unit, Tehran-Iran

Nezhad

Meybodi

Rezaee

et al. 2017

Jundishapur J Microbiol

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Background: Staphylococcus aureus is an important opportunistic pathogen and can cause a wide range of infections. The ability of this pathogen to successfully persist within the hospital and the community is largely due to its remarkable ability to acquire resistance against various antimicrobial agents. Objectives: The aim of this study was to determine the carriage of antibacterial resistance genes and virulence markers of S. aureus isolates from hospitalized patients in intensive care units in Tehran, the capital of Iran. Methods: In this cross-sectional study that was conducted during an 11-month period since March 2015 to January 2016, a total of 105 S. aureus strains were investigated. MRSA screening was performed by phenotypic and genotypic methods. The Kirby-Bauer disk diffusion method was used to assess the sensitivity of S. aureus strains. The strains were typed based on the polymorphisms in SCCmec types. The presence of resistance (ermA, ermB, ermC, mupA, msrA, msrB, tetM, ant (4´)-Ia, aac (6´)-Ie/aph (2˝), aph (3´)-IIIa) and toxin (etb, eta, pvl, tst) encoding genes were investigated by the polymerase chain reaction (PCR) technique. Results: In this study, 105 isolates of S. aureus were obtained from 299 various clinical specimens. Ninety five (90.5%) strains were confirmed as methicillin-resistant S. aureus. The lowest levels of resistance were related to quinupristin-dalfopristin (16.8%) while the highest levels of resistance were related to penicillin (94.7%). Multi-drug resistance was observed in 91.5% of the isolates. Type IV was the most prevalent SCCmec type (57.9%), followed by type III (22.1), type V (12.6%), I (5.3%), and II (2.1%). Overall, 25 isolates (26.3%) harbored PVL-encoding genes, and all of them belonged to SCCmec type IV. The presence of resistance genes ant(4´) ermA, ermB, ermC, msrA, msrB, and tetM was detected in 94.7%, 81.1%, 31.6%, 31.6%, 15.9%, 18.9%, 47.3%, 21.1%, 56.8%. The frequency of the etb, eta, and tst genes were 1.1%, 4.2%, and 32.6%, respectively. Conclusions:The results illustrated the diversity of antibacterial resistance and virulence gene profiles among different SCCmec types of S. aureus. The increased prevalence of methicillin-resistant S. aureus isolates containing different toxin and antibiotic resistance genes is a serious threat for the hospitalized patients in the intensive care units.

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Characterization of methicillin‐resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children

et al. 2021

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Characterisation of virulence genes in methicillin susceptible and resistant Staphylococcus aureus isolates from a paediatric population in a university hospital of Medellín, Colombia

Abstract: Virulence and antibiotic resistance are significant determinants of the types of infections caused by

Cited by 53 publications

References 38 publications

Distribution of Adhesion and Toxin Genes in Staphylococcus aureus Strains Recovered From Hospitalized Patients Admitted to the ICU

Distribution of Adhesion and Toxin Genes in Staphylococcus aureus Strains Recovered From Hospitalized Patients Admitted to the ICU

Molecular Characterization and Resistance Profile of Methicillin Resistant Staphylococcus aureus Strains Isolated from Hospitalized Patients in Intensive Care Unit, Tehran-Iran

Characterization of methicillin‐resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children

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