Trypanosoma cruzi: parasite persistence in tissues in chronic chagasic Brazilian patients

Marcon, Gláucia Elisete Barbosa; Albuquerque, Dulcinéia Martins; Batista, Angélica Martins; Andrade, Paula Durante; Almeida, Eros Antônio de; Guariento, Maria Elena; Teixeira, Maria Aparecida Barone; Costa, Sandra Cecı́lia Botelho

doi:10.1590/s0074-02762011000100014

Cited by 45 publications

(28 citation statements)

References 29 publications

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“…It is interesting to note, however, that most of the previous studies detected T. cruzi in cardiac tissue and not in isolated cardiomyocytes. 30,31 Here, we detected the presence of T. cruzi in isolated cardiomyocytes, which strongly indicates that parasites are present in cardiomyocyte cells. However, we cannot completely rule out the presence of infected cells isolated along with cardiomyocytes during the enzymatic dissociation of the heart.…”

Section: Discussionmentioning

confidence: 54%

Altered Cardiomyocyte Function and Trypanosoma cruzi Persistence in Chagas Disease

Cruz¹,

Santos‐Miranda²,

Sales-Junior³

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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Abstract. Chagas disease, caused by the triatominae Trypanosoma cruzi, is one of the leading causes of heart malfunctioning in Latin America. The cardiac phenotype is observed in 20-30% of infected people 10-40 years after their primary infection. The cardiac complications during Chagas disease range from cardiac arrhythmias to heart failure, with important involvement of the right ventricle. Interestingly, no studies have evaluated the electrical properties of right ventricle myocytes during Chagas disease and correlated them to parasite persistence. Taking advantage of a murine model of Chagas disease, we studied the histological and electrical properties of right ventricle in acute (30 days postinfection [dpi]) and chronic phases (90 dpi) of infected mice with the Colombian strain of T. cruzi and their correlation to parasite persistence. We observed an increase in collagen deposition and inflammatory infiltrate at both 30 and 90 dpi. Furthermore, using reverse transcriptase polymerase chain reaction, we detected parasites at 90 dpi in right and left ventricles. In addition, we observed action potential prolongation and reduced transient outward K + current and L-type Ca 2+ current at 30 and 90 dpi. Taking together, our results demonstrate that T. cruzi infection leads to important modifications in electrical properties associated with inflammatory infiltrate and parasite persistence in mice right ventricle, suggesting a causal role between inflammation, parasite persistence, and altered cardiomyocyte function in Chagas disease. Thus, arrhythmias observed in Chagas disease may be partially related to altered electrical function in right ventricle.

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Section: Discussionmentioning

confidence: 54%

Altered Cardiomyocyte Function and Trypanosoma cruzi Persistence in Chagas Disease

Cruz¹,

Santos‐Miranda²,

Sales-Junior³

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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“…Furthermore, parasite load in heart and muscle determined by RT-PCR was ∼5-fold lower in mTS-IMX immunized mice than in the infected control. These results are highly promising, taking into account that in human Chagas disease, the presence of inflammatory infiltrate in the heart was correlated with parasite persistence [51,52]. Additionally, TS-stimulated splenocytes from infected mTS-IMX mice produced the highest levels of IFN-␥ and IL-10 of all groups, suggesting a highly inflammatory but controlled response.…”

Section: Discussionmentioning

confidence: 79%

Efficacy of a trans-sialidase-ISCOMATRIX subunit vaccine candidate to protect against experimental Chagas disease

Bontempi

Vicco

Cabrera

et al. 2015

Vaccine

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“…The data presented here also show that the 3973 epitope is not recognized by the sera from individuals with SLE, rheumatoid arthritis, or celiac disease or patients with a nonchagasic cardiomyopathy. The results suggest the presence of the parasite in tissues from chronic ChD patients and its essential role in the pathogenesis of the chronic phase of this disease (20,28,33).…”

Section: Discussionmentioning

confidence: 80%

Characterization of an Immunodominant Antigenic Epitope from Trypanosoma cruzi as a Biomarker of Chronic Chagas' Disease Pathology

Thomas

Fernández-Villegas

Carrilero

et al. 2012

Clin Vaccine Immunol

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Nowadays, the techniques available for chronic Chagas' disease diagnosis are very sensitive; however, they do not allow discrimination of the patient's clinical stages of the disease. The present paper describes that three out of the five different repeats contained in the Trypanosoma cruzi TcCA-2 membrane protein (3972-FGQAAAGDKPPP, 6303-FGQAAAGDKPAP, and 3973-FGQ AAAGDKPSL) are recognized with high sensitivity (>90%) by sera from chronic Chagas' disease patients and that they are not recognized by sera from patients in the acute phase of the disease. A total of 133 serum samples from chagasic patients and 50 serum samples from healthy donors were tested. In addition, sera from 15 patients with different autoimmune diseases, 43 serum samples from patients suffering an infectious disease other than Chagas' disease, and 38 serum samples from patients with nonchagasic cardiac disorders were also included in this study. The residue 3973 peptide shows a specificity of >98%, as it is not recognized by individuals with autoimmune and inflammatory processes or by patients with a nonchagasic cardiomyopathy. Remarkably, the levels of antibody against the 3973 epitope detected by the sera from Chagas' disease patients in the symptomatic chronic phase, involving cardiac or digestive alterations, are higher than those detected by the sera from Chagas' disease patients in the indeterminate phase of the disease. It is suggested that the diagnostic technique described could also be used to indicate the degree of pathology. The amino acids F, Q, and DKP located in the peptide at positions 1, 3, and 8 to 10, respectively, are essential to conform to the immunodominant antigenic epitope.

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Trypanosoma cruzi: parasite persistence in tissues in chronic chagasic Brazilian patients

Cited by 45 publications

References 29 publications

Altered Cardiomyocyte Function and Trypanosoma cruzi Persistence in Chagas Disease

Altered Cardiomyocyte Function and Trypanosoma cruzi Persistence in Chagas Disease

Efficacy of a trans-sialidase-ISCOMATRIX subunit vaccine candidate to protect against experimental Chagas disease

Characterization of an Immunodominant Antigenic Epitope from Trypanosoma cruzi as a Biomarker of Chronic Chagas' Disease Pathology

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